LOCUS       RC213239                8538 bp    DNA     circular  SYN 
DEFINITION  NRD1 (Myc-DDK-tagged)-Human nardilysin (N-arginine dibasic convertase) (NRD1), transcript variant 1
ACCESSION   NM_002525
VERSION     NM_002525.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="NRDC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAGGAGA GTCACTGTTG CTGCAGTCTG TGCCACCCGG AGGAAGTTGT 
     1081 GTGAGGCCGG GCGGGACGTC GCGGCGCTCT GGGGAATCGA AACGCGGGGT CGGTGCGAAG 
     1141 ACTCTGCTGC TGCCAGACCC TTTCCTATTC TGGCCATGCC TGGAAGGAAC AAGGCGAAGT 
     1201 CTACCTGCAG CTGCCCTGAC CTGCAGCCCA ATGGACAGGA TCTGGGCGAG AACAGCCGGG 
     1261 TTGCCCGTCT AGGAGCGGAT GAATCTGAGG AAGAGGGACG GAGGGGGTCT CTCAGTAATG 
     1321 CTGGGGACCC TGAGATCGTC AAGTCTCCCA GCGACCCCAA GCAATACCGA TACATCAAAT 
     1381 TACAGAATGG CCTACAGGCA CTTCTGATTT CAGACCTAAG TAATATGGAA GGTAAAACAG 
     1441 GAAATACAAC AGATGATGAA GAAGAAGAGG AGGTGGAGGA AGAAGAAGAA GATGATGATG 
     1501 AAGATTCTGG AGCTGAAATA GAAGATGACG ATGAAGAGGG TTTTGATGAT GAAGATGAGT 
     1561 TTGATGATGA ACATGATGAT GATCTTGATA CTGAGGATAA TGAATTGGAA GAATTAGAAG 
     1621 AGAGAGCAGA AGCTAGAAAA AAAACTACTG AAAAACAGCA ATTGCAGAGC CTGTTTTTGC 
     1681 TGTGGTCAAA GCTGACTGAT AGACTGTGGT TTAAGTCAAC TTATTCAAAA ATGTCTTCAA 
     1741 CCCTGCTGGT CGAGACAAGA AATCTTTATG GGGTAGTTGG AGCTGAAAGC AGGTCTGCAC 
     1801 CTGTTCAGCA TTTGGCAGGA TGGCAAGCGG AGGAGCAGCA GGGTGAAACT GACACAGTTC 
     1861 TGTCTGCAGC GGCTCTTTGT GTTGGAGTTG GGAGTTTCGC TGATCCAGAT GACCTGCCGG 
     1921 GGCTGGCACA CTTTTTGGAG CACATGGTAT TCATGGGTAG TTTGAAATAT CCAGATGAGA 
     1981 ATGGATTTGA TGCCTTCCTG AAGAAGCATG GGGGTAGTGA TAATGCCTCA ACTGATTGTG 
     2041 AACGCACTGT CTTTCAGTTT GATGTCCAGA GGAAGTACTT CAAGGAAGCT CTTGATAGAT 
     2101 GGGCGCAGTT CTTCATCCAC CCACTAATGA TCAGAGATGC AATTGACCGT GAAGTTGAAG 
     2161 CTGTTGATAG TGAATATCAA CTTGCAAGGC CTTCTGATGC AAACAGAAAG GAAATGTTGT 
     2221 TTGGAAGCCT TGCTAGACCT GGCCATCCTA TGGGAAAATT TTTTTGGGGA AATGCTGAGA 
     2281 CGCTCAAGCA TGAGCCAAGA AAGAATAATA TTGATACACA TGCTAGATTG AGAGAATTCT 
     2341 GGATGCGTTA CTACTCTTCT CATTACATGA CTTTAGTGGT TCAATCCAAA GAAACACTGG 
     2401 ATACTTTGGA AAAGTGGGTG ACTGAAATCT TCTCTCAGAT ACCAAACAAT GGGTTACCCA 
     2461 GACCAAACTT TGGCCATTTA ACGGATCCAT TTGACACACC AGCATTTAAC AAACTTTATA 
     2521 GAGTTGTTCC AATCAGAAAA ATTCATGCTC TGACCATCAC ATGGGCACTT CCTCCTCAAC 
     2581 AGCAACATTA CAGGGTGAAG CCACTTCATT ATATATCCTG GCTGGTTGGA CATGAAGGCA 
     2641 AAGGCAGCAT TCTTTCTTTC CTTAGGAAAA AATGCTGGGC TCTTGCACTG TTTGGTGGAA 
     2701 ATGGTGAGAC AGGATTTGAG CAAAATTCTA CTTATTCAGT GTTCAGCATT TCTATTACAT 
     2761 TGACTGATGA GGGTTATGAA CATTTTTATG AGGTTGCTTA CACTGTCTTT CTGTATTTAA 
     2821 AAATGCTGCA GAAGCTAGGC CCAGAAAAAA GAATTTTTGA AGAGATTCGG AAAATTGAGG 
     2881 ATAATGAATT TCATTACCAA GAACAGACAG ATCCAGTTGA GTATGTGGAA AACATGTGTG 
     2941 AGAACATGCA GCTGTACCCA TTGCAGGACA TTCTCACTGG AGATCAGCTT CTTTTTGAAT 
     3001 ACAAGCCAGA AGTCATTGGT GAAGCCTTGA ATCAGCTAGT TCCTCAAAAA GCAAATCTTG 
     3061 TTTTACTGTC TGGTGCTAAT GAGGGAAAAT GTGACCTCAA GGAGAAATGG TTTGGAACTC 
     3121 AATATAGTAT AGAAGATATT GAAAACTCTT GGGCTGAACT GTGGAATAGT AATTTCGAAT 
     3181 TAAATCCAGA TCTTCATCTT CCAGCTGAAA ACAAGTACAT AGCCACGGAC TTTACGTTGA 
     3241 AGGCTTTCGA TTGCCCGGAA ACAGAATACC CAGTTAAAAT TGTGAATACT CCACAAGGTT 
     3301 GCCTGTGGTA TAAGAAAGAC AACAAATTCA AAATCCCCAA AGCATATATA CGTTTCCATC 
     3361 TAATTTCACC GTTGATACAG AAATCTGCAG CAAATGTGGT CCTCTTTGAT ATCTTTGTCA 
     3421 ATATCCTTAC GCATAACCTT GCGGAACCAG CTTATGAAGC AGATGTGGCA CAGCTGGAGT 
     3481 ATAAACTGGC AGCTGGAGAA CATGGTTTAA TTATTCGAGT GAAAGGATTT AACCACAAAC 
     3541 TACCTCTACT GTTTCAGCTC ATTATTGACT ACTTAGCTGA GTTCAATTCC ACACCAGCTG 
     3601 TCTTTACAAT GATAACTGAG CAGTTGAAGA AGACCTACTT TAACATCCTC ATCAAGCCTG 
     3661 AGACTTTGGC CAAAGATGTA CGGCTTTTAA TCTTGGAATA TGCCCGTTGG TCTATGATTG 
     3721 ACAAGTACCA GGCTTTGATG GACGGCCTTT CCCTTGAGTC TCTGCTGAGC TTCGTCAAAG 
     3781 AATTCAAATC CCAGCTCTTT GTGGAGGGCC TGGTACAAGG GAATGTCACA AGCACAGAAT 
     3841 CTATGGATTT CCTGAAATAT GTTGTTGACA AACTAAACTT CAAGCCTCTG GAGCAGGAGA 
     3901 TGCCTGTGCA GTTCCAGGTG GTAGAGCTGC CCAGTGGCCA CCATCTATGC AAAGTGAAAG 
     3961 CTCTGAACAA GGGTGATGCC AACTCTGAAG TCACTGTGTA CTACCAGTCA GGTACCAGGA 
     4021 GTCTAAGAGA ATATACGCTT ATGGAGCTGC TTGTGATGCA CATGGAAGAA CCTTGTTTTG 
     4081 ACTTCCTTCG AACCAAGCAG ACCCTTGGGT ACCATGTCTA CCCTACCTGT AGGAACACAT 
     4141 CCGGGATTCT AGGATTTTCT GTCACTGTGG GGACTCAGGC AACCAAATAC AATTCTGAAG 
     4201 TTGTTGATAA GAAGATAGAA GAGTTTCTTT CTAGCTTTGA GGAGAAGATT GAGAACCTCA 
     4261 CTGAAGAGGC ATTCAACACC CAGGTCACAG CTCTCATCAA GCTGAAGGAG TGTGAGGATA 
     4321 CCCACCTTGG GGAGGAGGTG GATAGGAACT GGAATGAAGT GGTTACACAG CAGTACCTCT 
     4381 TTGACCGCCT TGCCCACGAG ATTGAAGCAC TGAAGTCATT CTCAAAATCA GACCTGGTCA 
     4441 ACTGGTTCAA GGCTCATAGA GGGCCAGGAA GTAAAATGCT CAGCGTTCAT GTTGTTGGGT 
     4501 ATGGGAAGTA TGAACTGGAA GAGGATGGAT CCCCTTCTAG TGAGGATTCA AATTCTTCTT 
     4561 GTGAAGTGAT GCAGCTGACC TACCTGCCAA CCTCTCCTCT GCTGGCAGAT TGTATCATCC 
     4621 CCATTACTGA TATCAGGGCT TTCACAACAA CACTCAACCT TCTCCCCTAC CATAAAATAG 
     4681 TCAAAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//