LOCUS       RC213221                8823 bp    DNA     circular  SYN 
DEFINITION  RNF123 (Myc-DDK-tagged)-Human ring finger protein 123 (RNF123)
ACCESSION   NM_022064
VERSION     NM_022064.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4980..5071 
                     /label="Myc-DDK"  
     misc_feature    5101..5686 
                     /label="PolyA signal"  
     misc_feature    5158..5177 
                     /label="Restriction Enzyme"  
     misc_feature    5962..6634 
                     /label="ColE1"  
     misc_feature    7064..7858 
                     /label="Kan/Neo"  
     misc_feature    7893..8195 
                     /label="SV40 ori"  
     misc_feature    8281..8758 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4962..4968 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4962 
                     /label="RNF123"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCCAAG GGGGCCGGCA TGTCTTTCTC CCGCAAGAGC TATAGGCTGA 
     1081 CCTCAGATGC TGAGAAATCC AGGGTCACAG GCATTGTGCA GGAGAAGCTG CTGAATGACT 
     1141 ACCTGAACCG CATCTTTTCC TCTTCTGAAC ATGCACCCCC AGCAGCCACC AGCAGGAAAC 
     1201 CCCTGAACTT CCAGAACCTG CCAGAACATT TGGACCAGTT GCTACAGGTG GACAATGAGG 
     1261 AGGAGGAAAG CCAGGGACAG GTTGAAGGGC GGCTTGGCCC ATCCACTGTG GTCCTGGACC 
     1321 ACACAGGCGG CTTTGAGGGG CTTCTCCTGG TGGATGATGA CCTGCTGGGG GTGATTGGAC 
     1381 ACAGCAACTT TGGCACCATC CGCTCTACCA CATGCGTGTA CAAAGGGAAA TGGCTCTACG 
     1441 AGGTCCTCAT CTCCTCCCAG GGGCTCATGC AGATCGGCTG GTGCACCATC AGCTGCCGCT 
     1501 TCAACCAGGA GGAGGGGGTT GGAGATACAC ACAACTCCTA TGCCTATGAT GGCAACCGCG 
     1561 TGCGCAAGTG GAATGTGACC ACAACGAATT ATGGCAAGGC GTGGGCAGCG GGGGACATCG 
     1621 TGAGCTGCCT GATTGACCTG GATGATGGCA CTCTGTCCTT CTGCCTGAAC GGTGTATCAC 
     1681 TGGGCACTGC CTTTGAGAAC CTGTCCAGGG GCCTGGGTAT GGCCTACTTC CCAGCCATCA 
     1741 GCCTCTCTTT CAAGGAGTCC GTGGCCTTCA ACTTTGGCAG CCGTCCTCTG CGCTACCCAG 
     1801 TGGCAGGCTA CCGGCCCCTG CAGGACCCAC CGAGTGCTGA CCTGGTGCGG GCACAGAGGT 
     1861 TGCTGGGCTG CTTCCGGGCA GTGCTGAGTG TGGAGCTGGA CCCTGTGGAG GGGCGGCTGT 
     1921 TGGACAAGGA GAGCTCCAAG TGGCGGTTGC GGGGCCAGCC CACCGTCCTC CTCACACTGG 
     1981 CCCACATCTT CCATCACTTT GCACCGCTTC TGCGCAAGGT GTATCTGGTG GAGGCTGTGC 
     2041 TCATGAGCTT CTTGCTGGGC ATCGTGGAGA AGGGCACACC CACACAGGCA CAGTCCGTGG 
     2101 TGCACCAGGT CCTGGACCTC TTGTGGCTCT TCATGGAGGA CTACGAGGTA CAAGATTGCC 
     2161 TCAAGCAGTT GATGATGTCT CTGCTTCGGC TGTACCGATT CTCACCCATT GTCCCAGACC 
     2221 TGGGCCTACA GATCCATTAC CTGCGGCTCA CTATCGCCAT CCTGAGGCAT GAGAAGTCCC 
     2281 GCAAGTTTCT GCTTAGCAAT GTCCTCTTCG ACGTGCTCCG CTCCGTCGTC TTCTTTTACA 
     2341 TCAAGAGCCC CCTGCGTGTG GAGGAGGCCG GCCTGCAGGA GCTCATTCCC ACCACCTGGT 
     2401 GGCCCCACTG CTCCAGTAGG GAGGGCAAAG AGAGCACGGA GATGAAGGAG GAGACCGCAG 
     2461 AGGAGCGGCT GCGGCGGCGA GCCTACGAAC GGGGCTGTCA GCGGCTCAGG AAGCGCATCG 
     2521 AAGTGGTGGA AGAACTACAG GTCCAGATCC TGAAGCTGCT GCTGGACAAT AAAGATGACA 
     2581 ATGGGGGTGA AGCTTCTAGG TATATCTTCC TGACCAAGTT TCGCAAGTTT CTGCAGGAGA 
     2641 ACGCCAGTGG CCGGGGGAAC ATGCCCATGC TCTGCCCCCC TGAGTACATG GTCTGCTTCT 
     2701 TACACCGGCT GATCTCTGCC CTGCGCTACT ATTGGGATGA ATACAAGGCT TCCAATCCTC 
     2761 ATGCTTCCTT CAGTGAGGAG GCCTACATCC CGCCCCAGGT CTTCTATAAT GGCAAGGTGG 
     2821 ACTACTTTGA CCTGCAGCGC CTGGGGGGCC TCCTCTCGCA CCTGCGGAAG ACCCTCAAAG 
     2881 ATGACCTTGC TTCCAAAGCC AACATTGTGA TCGACCCACT GGAGCTCCAG TCAACCGCCA 
     2941 TGGATGACCT AGATGAGGAT GAGGAGCCAG CCCCAGCTAT GGCCCAGCGC CCCATGCAGG 
     3001 CCCTGGCTGT TGGGGGGCCA CTGCCCCTGC CCCGGCCCGG CTGGCTCAGT TCTCCAACTT 
     3061 TGGGCCGAGC CAACCGCTTC CTCAGCACAG CGGCTGTGAG CCTCATGACC CCACGGCGGC 
     3121 CTCTGAGCAC CTCGGAGAAA GTGAAGGTCC GCACGCTGAG CGTGGAGCAG AGGACCCGTG 
     3181 AGGACATTGA AGGCAGCCAC TGGAATGAGG GCTTGCTGCT GGGGCGGCCC CCCGAGGAGC 
     3241 CTGAGCAGCC CCTCACCGAG AACTCGCTGC TGGAAGTCCT GGATGGGGCG GTCATGATGT 
     3301 ACAACCTCAG CGTACACCAG CAGCTGGGCA AGATGGTGGG TGTCTCCGAT GATGTCAATG 
     3361 AATACGCTAT GGCTCTGAGG GACACAGAGG ACAAGCTCCG CCGGTGCCCC AAGAGGAGGA 
     3421 AGGACATCCT TGCAGAGTTG ACCAAGAGCC AGAAGGTTTT CTCAGAAAAG CTGGACCACC 
     3481 TGAGCCGCCG TCTTGCCTGG GTCCATGCCA CTGTCTACTC CCAGGAGAAG ATGCTGGACA 
     3541 TCTACTGGCT GCTGCGCGTC TGCCTGCGGA CCATTGAGCA CGGTGATCGC ACAGGGTCTC 
     3601 TCTTTGCCTT CATGCCCGAG TTCTACCTGA GCGTGGCCAT CAACAGCTAC AGTGCTCTCA 
     3661 AGAATTACTT TGGTCCCGTG CACAGCATGG AGGAGCTCCC AGGCTATGAA GAGACCCTGA 
     3721 CCCGCCTGGC TGCCATTCTC GCCAAACACT TTGCCGACGC ACGCATTGTG GGCACTGACA 
     3781 TCCGAGACTC ACTGATGCAG GCCCTGGCCA GCTACGTGTG CTACCCACAC TCCCTGCGGG 
     3841 CTGTGGAGCG AATCCCCGAG GAGCAGCGTA TCGCCATGGT GAGGAACCTC CTGGCGCCCT 
     3901 ATGAGCAGCG GCCCTGGGCC CAGACCAACT GGATCCTGGT GCGGCTCTGG AGGGGCTGTG 
     3961 GCTTCGGGTA CCGCTATACA CGGCTGCCAC ATCTGCTGAA AACCAAACTT GAGGACGCCA 
     4021 ATTTGCCCAG CCTCCAGAAG CCCTGCCCTT CCACCCTGCT GCAGCAGCAC ATGGCGGACC 
     4081 TCCTACAGCA GGGTCCTGAT GTGGCACCCA GCTTCCTCAA CAGCGTCCTC AATCAGCTCA 
     4141 ACTGGGCCTT CTCTGAATTC ATTGGCATGA TCCAAGAGAT CCAGCAGGCT GCTGAGCGCC 
     4201 TGGAGCGGAA CTTTGTGGAC AGCCGGCAGC TCAAGGTATG TGCCACCTGC TTTGACCTCT 
     4261 CGGTCAGCCT GCTGCGTGTC TTGGAGATGA CTATCACACT GGTGCCTGAG ATATTCCTTG 
     4321 ACTGGACCCG GCCTACCTCT GAGATGCTGC TGCGGCGTCT TGCACAGCTG CTAAACCAGG 
     4381 TGCTGAACCG GGTGACAGCT GAGAGGAACC TGTTTGATCG TGTGGTCACC CTACGGCTGC 
     4441 CTGGCCTAGA GAGCGTGGAC CACTATCCCA TTCTGGTGGC AGTGACGGGC ATCCTGGTGC 
     4501 AGCTCCTGGT GCGTGGCCCA GCCTCAGAGA GAGAGCAAGC CACATCAGTG CTCCTGGCAG 
     4561 ATCCCTGCTT CCAGCTACGC TCAATATGCT ATCTCCTGGG ACAGCCAGAG CCCCCAGCAC 
     4621 CTGGCACTGC TCTGCCAGCC CCTGACCGGA AGCGCTTCTC CCTGCAGAGC TATGCGGATT 
     4681 ATATCAGTGC CGATGAGCTG GCCCAAGTGG AACAGATGCT GGCGCACCTG ACCTCTGCAT 
     4741 CTGCCCAGGC AGCAGCTGCC TCCCTGCCCA CCAGTGAGGA GGACCTCTGC CCCATCTGCT 
     4801 ATGCCCACCC CATCTCTGCT GTGTTCCAGC CCTGTGGCCA CAAGTCCTGC AAAGCCTGTA 
     4861 TCAACCAGCA CCTGATGAAC AACAAGGACT GCTTCTTCTG CAAAACCACC ATCGTGTCTG 
     4921 TAGAGGACTG GGAGAAGGGA GCCAATACGA GTACTACCTC CTCAGCTGCC ACGCGTACGC 
     4981 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5041 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5101 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5161 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5221 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5281 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5341 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5401 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5461 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5521 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5581 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5641 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5701 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5761 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5821 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5881 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5941 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6001 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6061 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6121 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6181 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6241 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6301 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6361 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6421 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6481 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6541 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6601 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6661 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6721 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6781 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6841 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6901 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6961 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7021 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7081 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7141 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7201 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7261 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7321 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7381 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7441 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7501 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7561 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7621 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7681 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7741 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7801 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7861 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7921 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7981 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8041 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8221 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8281 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8341 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8401 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8461 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8521 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8581 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8641 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8701 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8761 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8821 TTT
//