LOCUS       RC213189                8841 bp    DNA     circular  SYN 
DEFINITION  MYPN (Myc-DDK-tagged)-Human myopalladin (MYPN)
ACCESSION   NM_032578
VERSION     NM_032578.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="MYPN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAAGACGAC AGCATAGAAG CTTCTACTTC CATATCTCAG CTTCTAAGAG 
     1081 AGAGCTATTT AGCTGAAACC AGACATCGGG GAAACAATGA GAGGAGTCGA GCGGAGCCCT 
     1141 CCTCCAACCC TTGCCATTTC GGCAGTCCTT CTGGGGCCGC TGAAGGAGGC GGAGGCCAAG 
     1201 ATGACCTTCC AGATCTTTCA GCCTTTCTGA GCCAAGAAGA ATTAGACGAA AGTGTCAATT 
     1261 TGGCAAGACT GGCCATCAAT TACGACCCTT TGGAGAAGGC AGATGAAACT CAAGCTAGAA 
     1321 AACGACTTTC TCCTGATCAG ATGAAACACT CACCTAATTT AAGTTTTGAG CCTAACTTCT 
     1381 GCCAGGATAA CCCTCGAAGT CCCACCAGCT CTAAAGAAAG CCCCCAGGAG GCAAAAAGGC 
     1441 CACAGTATTG TTCTGAAACC CAGTCCAAAA AAGTATTTTT AAATAAGGCT GCCGACTTCA 
     1501 TTGAAGAGCT ATCCTCCCTT TTCAAATCCC ACAGCTCCAA AAGGATTAGA CCTCGTGCCT 
     1561 GCAAAAACCA CAAGAGTAAA CTGGAATCTC AAAACAAAGT TATGCAGGAA AACAGCTCCA 
     1621 GTTTCTCAGA TCTGTCAGAA AGACGAGAAA GATCTTCTGT TCCCATCCCT ATCCCTGCGG 
     1681 ATACCAGGGA TAATGAAGTG AATCACGCCC TGGAACAGCA GGAAGCCAAG AGGCGTGAAG 
     1741 CGGAGCAGGC TGCCAGTGAG GCGGCTGGTG GAGACACTAC ACCAGGGTCT TCCCCTTCAT 
     1801 CTCTGTACTA TGAAGAACCT CTGGGGCAAC CTCCCCGGTT CACTCAAAAG TTACGGAGCA 
     1861 GAGAAGTTCC AGAAGGAACT CGAGTACAGT TGGATTGCAT AGTGGTAGGA ATTCCACCAC 
     1921 CTCAAGTAAG GTGGTACTGT GAAGGCAAGG AGCTTGAAAA TTCCCCAGAT ATTCACATCG 
     1981 TCCAGGCAGG AAATCTGCAC TCACTGACCA TTGCGGAAGC CTTTGAAGAG GACACAGGAC 
     2041 GCTATTCCTG CTTTGCTTCT AACATCTATG GGACAGATTC GACTTCTGCT GAGATTTATA 
     2101 TAGAAGGGGT TTCTTCTTCT GACTCAGAAG GCGACCCTAA CAAGGAAGAG ATGAATCGAA 
     2161 TCCAGAAGCC AAATGAGGTG TCATCTCCTC CCACTACCTC TGCAGTCATT CCTCCAGCAG 
     2221 TACCCCAAGC CCAGCATTTG GTGGCCCAAC CTCGTGTGGC AACCATCCAG CAGTGTCAGA 
     2281 GCCCCACCAA TTACTTGCAG GGATTGGATG GAAAACCTAT CATTGCAGCT CCTGTGTTTA 
     2341 CAAAGATGCT ACAAAATTTG TCAGCTTCTG AGGGTCAGCT GGTTGTCTTT GAATGCAGAG 
     2401 TAAAAGGAGC TCCATCTCCT AAGGTTGAGT GGTATAGAGA AGGGACTTTA ATAGAAGATT 
     2461 CTCCAGATTT TAGGATTTTA CAGAAAAAAC CTCGATCCAT GGCAGAGCCA GAGGAGATTT 
     2521 GCACCTTGGT CATTGCTGAG GTGTTTGCAG AAGATTCTGG GTGCTTCACA TGTACTGCAA 
     2581 GCAACAAATA CGGCACAGTG TCAAGCATTG CACAGCTGCA CGTGAGAGGA AATGAGGACC 
     2641 TCAGCAACAA CGGGTCTCTT CACTCAGCCA ACTCTACCAC CAACCTGGCA GCTATTGAGC 
     2701 CACAGCCCTC CCCACCCCAC TCAGAGCCTC CATCTGTGGA ACAACCCCCC AAACCCAAAC 
     2761 TCGAGGGGGT TCTGGTGAAC CACAATGAGC CCCGGTCCAG CTCCAGGATT GGGCTTCGTG 
     2821 TGCACTTCAA CCTGCCTGAA GATGACAAAG GAAGTGAAGC ATCCTCCGAG GCTGGTGTGG 
     2881 TGACCACCAG ACAGACCAGG CCCGATTCTT TCCAGGAGAG GTTCAACGGA CAGGCAACAA 
     2941 AAACCCCAGA GCCTTCTTCC CCCGTGAAAG AGCCCCCTCC AGTTCTGGCC AAACCCAAAC 
     3001 TTGATTCCAC TCAGTTACAA CAGCTTCATA ACCAAGTCTT ACTGGAACAA CACCAATTGC 
     3061 AAAACCCACC TCCTTCATCT CCTAAGGAGT TTCCTTTCAA CATGACTGTT TTGAACTCCA 
     3121 ATGCTCCCCC AGCGGTGACA ACATCCAATA AGCAGGTGAA GGCTCCTTCA TCACAGACGT 
     3181 TCAGCTTGGC CCGGCCGAAG TATTTCTTCC CCTCCACGAA CACCACCGCA GCAACTGTGG 
     3241 CCCCTTCCAG CTCTCCGGTG TTCACTTTGA GCAGCACTCC TCAAACTATT CAGAGGACAG 
     3301 TGAGCAAAGA AAGCCTCTTA GTGTCTCACC CCTCTGTGCA AACCAAATCT CCAGGAGGGC 
     3361 TTTCCATCCA AAATGAGCCA CTCCCACCAG GCCCAACAGA ACCAACACCA CCACCATTCA 
     3421 CATTTTCCAT CCCCAGGGGA AACCAGTTTC AGCCCCGCTG TGTGTCCCCA ATTCCTGTCT 
     3481 CTCCTACCAG CCGGATTCAG AACCCAGTGG CTTTCCTCAG CTCTGTTCTG CCTTCTCTCC 
     3541 CTGCCATCCC ACCCACAAAT GCCATGGGGC TGCCTAGAAG TGCACCATCC ATGCCATCCC 
     3601 AGGGATTAGC GAAGAAAAAT ACAAAGTCTC CTCAACCAGT GAATGATGAT AACATTCGTG 
     3661 AAACTAAGAA CGCAGTGATT CGAGACTTGG GAAAAAAAAT AACTTTCAGT GATGTCAGAC 
     3721 CAAACCAGCA GGAGTACAAA ATTTCAAGCT TTGAGCAGAG GCTGATGAAT GAAATAGAGT 
     3781 TTCGCTTGGA ACGTACTCCT GTTGATGAAT CAGATGATGA AATTCAACAT GATGAGATCC 
     3841 CCACGGGCAA GTGTATTGCT CCCATCTTTG ACAAGAGACT CAAGCACTTC CGGGTCACAG 
     3901 AAGGCTCTCC AGTCACATTC ACCTGCAAAA TTGTTGGGAT ACCTGTTCCA AAGGTTTACT 
     3961 GGTTCAAAGA TGGGAAGCAG ATTTCTAAGA GAAATGAGCA CTGCAAAATG AGGCGAGAAG 
     4021 GAGATGGGAC ATGCTCTCTG CACATTGAAT CCACTACCAG TGATGACGAT GGCAACTACA 
     4081 CCATCATGGC AGCCAACCCC CAGGGGAGAA TCAGCTGTTC TGGCCACTTG ATGGTACAAA 
     4141 GTTTGCCCAT TCGCAGTCGG CTAACCTCTG CTGGTCAGTC TCACAGGGGA AGATCCCGAG 
     4201 TGCAAGAAAG AGACAAAGAG CCCCTACAGG AACGCTTTTT CCGACCACAT TTCCTGCAGG 
     4261 CTCCTGGGGA TATGGTAGCT CATGAGGGGC GCCTCTGTCG GCTGGACTGT AAGGTGAGTG 
     4321 GTTTACCGCC CCCGGAGCTG ACATGGCTAC TCAATGGCCA ACCTGTGCTA CCAGATGCCT 
     4381 CCCACAAGAT GCTGGTCAGG GAGACCGGAG TCCACTCTCT GCTCATTGAC CCACTCACTC 
     4441 AGCGCGACGC AGGGACCTAT AAGTGCATCG CTACCAACAA AACCGGGCAG AATTCTTTTA 
     4501 GTCTGGAGCT CTCTGTAGTA GCCAAAGAGG TGAAGAAAGC ACCTGTGATC CTGGAGAAAC 
     4561 TACAGAACTG CGGTGTTCCC GAAGGCCACC CCGTGAGACT GGAGTGCCGC GTGATAGGCA 
     4621 TGCCCCCACC TGTGTTCTAC TGGAAGAAAG ACAATGAGAC CATCCCTTGC ACCAGAGAGA 
     4681 GGATCAGTAT GCACCAGGAC ACAACAGGGT ATGCCTGCCT TCTCATTCAG CCAGCCAAGA 
     4741 AATCAGACGC TGGATGGTAC ACGTTGTCAG CCAAGAATGA AGCCGGCATC GTGTCGTGCA 
     4801 CTGCCAGGCT GGATATATAC GCTCAGTGGC ACCATCAGAT CCCACCGCCC ATGTCTGTCC 
     4861 GGCCCAGTGG CAGTCGCTAC GGATCTCTCA CCAGTAAAGG ACTTGACATA TTTTCTGCCT 
     4921 TTTCCTCCAT GGAAAGCACG ATGGTGTATT CATGCTCTTC TCGGAGTGTA GTGGAGAGTG 
     4981 ATGAACTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//