LOCUS       RC213112                8085 bp    DNA     circular  SYN 
DEFINITION  PIK3CA (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA)
ACCESSION   NM_006218
VERSION     NM_006218.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4242..4333 
                     /label="Myc-DDK"  
     misc_feature    4363..4948 
                     /label="PolyA signal"  
     misc_feature    4420..4439 
                     /label="Restriction Enzyme"  
     misc_feature    5224..5896 
                     /label="ColE1"  
     misc_feature    6326..7120 
                     /label="Kan/Neo"  
     misc_feature    7155..7457 
                     /label="SV40 ori"  
     misc_feature    7543..8020 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4230 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="PIK3CA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCACGA CCATCATCAG GTGAACTGTG GGGCATCCAC TTGATGCCCC 
     1081 CAAGAATCCT AGTAGAATGT TTACTACCAA ATGGAATGAT AGTGACTTTA GAATGCCTCC 
     1141 GTGAGGCTAC ATTAATAACC ATAAAGCATG AACTATTTAA AGAAGCAAGA AAATACCCCC 
     1201 TCCATCAACT TCTTCAAGAT GAATCTTCTT ACATTTTCGT AAGTGTTACT CAAGAAGCAG 
     1261 AAAGGGAAGA ATTTTTTGAT GAAACAAGAC GACTTTGTGA CCTTCGGCTT TTTCAACCCT 
     1321 TTTTAAAAGT AATTGAACCA GTAGGCAACC GTGAAGAAAA GATCCTCAAT CGAGAAATTG 
     1381 GTTTTGCTAT CGGCATGCCA GTGTGTGAAT TTGATATGGT TAAAGATCCA GAAGTACAGG 
     1441 ACTTCCGAAG AAATATTCTG AACGTTTGTA AAGAAGCTGT GGATCTTAGG GACCTCAATT 
     1501 CACCTCATAG TAGAGCAATG TATGTCTATC CTCCAAATGT AGAATCTTCA CCAGAATTGC 
     1561 CAAAGCACAT ATATAATAAA TTAGATAAAG GGCAAATAAT AGTGGTGATC TGGGTAATAG 
     1621 TTTCTCCAAA TAATGACAAG CAGAAGTATA CTCTGAAAAT CAACCATGAC TGTGTACCAG 
     1681 AACAAGTAAT TGCTGAAGCA ATCAGGAAAA AAACTCGAAG TATGTTGCTA TCCTCTGAAC 
     1741 AACTAAAACT CTGTGTTTTA GAATATCAGG GCAAGTATAT TTTAAAAGTG TGTGGATGTG 
     1801 ATGAATACTT CCTAGAAAAA TATCCTCTGA GTCAGTATAA GTATATAAGA AGCTGTATAA 
     1861 TGCTTGGGAG GATGCCCAAT TTGATGTTGA TGGCTAAAGA AAGCCTTTAT TCTCAACTGC 
     1921 CAATGGACTG TTTTACAATG CCATCTTATT CCAGACGCAT TTCCACAGCT ACACCATATA 
     1981 TGAATGGAGA AACATCTACA AAATCCCTTT GGGTTATAAA TAGTGCACTC AGAATAAAAA 
     2041 TTCTTTGTGC AACCTACGTG AATGTAAATA TTCGAGACAT TGATAAGATC TATGTTCGAA 
     2101 CAGGTATCTA CCATGGAGGA GAACCCTTAT GTGACAATGT GAACACTCAA AGAGTACCTT 
     2161 GTTCCAATCC CAGGTGGAAT GAATGGCTGA ATTATGATAT ATACATTCCT GATCTTCCTC 
     2221 GTGCTGCTCG ACTTTGCCTT TCCATTTGCT CTGTTAAAGG CCGAAAGGGT GCTAAAGAGG 
     2281 AACACTGTCC ATTGGCATGG GGAAATATAA ACTTGTTTGA TTACACAGAC ACTCTAGTAT 
     2341 CTGGAAAAAT GGCTTTGAAT CTTTGGCCAG TACCTCATGG ATTAGAAGAT TTGCTGAACC 
     2401 CTATTGGTGT TACTGGATCA AATCCAAATA AAGAAACTCC ATGCTTAGAG TTGGAGTTTG 
     2461 ACTGGTTCAG CAGTGTGGTA AAGTTCCCAG ATATGTCAGT GATTGAAGAG CATGCCAATT 
     2521 GGTCTGTATC CCGAGAAGCA GGATTTAGCT ATTCCCACGC AGGACTGAGT AACAGACTAG 
     2581 CTAGAGACAA TGAATTAAGG GAAAATGACA AAGAACAGCT CAAAGCAATT TCTACACGAG 
     2641 ATCCTCTCTC TGAAATCACT GAGCAGGAGA AAGATTTTCT ATGGAGTCAC AGACACTATT 
     2701 GTGTAACTAT CCCCGAAATT CTACCCAAAT TGCTTCTGTC TGTTAAATGG AATTCTAGAG 
     2761 ATGAAGTAGC CCAGATGTAT TGCTTGGTAA AAGATTGGCC TCCAATCAAA CCTGAACAGG 
     2821 CTATGGAACT TCTGGACTGT AATTACCCAG ATCCTATGGT TCGAGGTTTT GCTGTTCGGT 
     2881 GCTTGGAAAA ATATTTAACA GATGACAAAC TTTCTCAGTA TTTAATTCAG CTAGTACAGG 
     2941 TCCTAAAATA TGAACAATAT TTGGATAACT TGCTTGTGAG ATTTTTACTG AAGAAAGCAT 
     3001 TGACTAATCA AAGGATTGGG CACTTTTTCT TTTGGCATTT AAAATCTGAG ATGCACAATA 
     3061 AAACAGTTAG CCAGAGGTTT GGCCTGCTTT TGGAGTCCTA TTGTCGTGCA TGTGGGATGT 
     3121 ATTTGAAGCA CCTGAATAGG CAAGTCGAGG CAATGGAAAA GCTCATTAAC TTAACTGACA 
     3181 TTCTCAAACA GGAGAAGAAG GATGAAACAC AAAAGGTACA GATGAAGTTT TTAGTTGAGC 
     3241 AAATGAGGCG ACCAGATTTC ATGGATGCTC TACAGGGCTT TCTGTCTCCT CTAAACCCTG 
     3301 CTCATCAACT AGGAAACCTC AGGCTTGAAG AGTGTCGAAT TATGTCCTCT GCAAAAAGGC 
     3361 CACTGTGGTT GAATTGGGAG AACCCAGACA TCATGTCAGA GTTACTGTTT CAGAACAATG 
     3421 AGATCATCTT TAAAAATGGG GATGATTTAC GGCAAGATAT GCTAACACTT CAAATTATTC 
     3481 GTATTATGGA AAATATCTGG CAAAATCAAG GTCTTGATCT TCGAATGTTA CCTTATGGTT 
     3541 GTCTGTCAAT CGGTGACTGT GTGGGACTTA TTGAGGTGGT GCGAAATTCT CACACTATTA 
     3601 TGCAAATTCA GTGCAAAGGC GGCTTGAAAG GTGCACTGCA GTTCAACAGC CACACACTAC 
     3661 ATCAGTGGCT CAAAGACAAG AACAAAGGAG AAATATATGA TGCAGCCATT GACCTGTTTA 
     3721 CACGTTCATG TGCTGGATAC TGTGTAGCTA CCTTCATTTT GGGAATTGGA GATCGTCACA 
     3781 ATAGTAACAT CATGGTGAAA GACGATGGAC AACTGTTTCA TATAGATTTT GGACACTTTT 
     3841 TGGATCACAA GAAGAAAAAA TTTGGTTATA AACGAGAACG TGTGCCATTT GTTTTGACAC 
     3901 AGGATTTCTT AATAGTGATT AGTAAAGGAG CCCAAGAATG CACAAAGACA AGAGAATTTG 
     3961 AGAGGTTTCA GGAGATGTGT TACAAGGCTT ATCTAGCTAT TCGACAGCAT GCCAATCTCT 
     4021 TCATAAATCT TTTCTCAATG ATGCTTGGCT CTGGAATGCC AGAACTACAA TCTTTTGATG 
     4081 ACATTGCATA CATTCGAAAG ACCCTAGCCT TAGATAAAAC TGAGCAAGAG GCTTTGGAGT 
     4141 ATTTCATGAA ACAAATGAAT GATGCACATC ATGGTGGCTG GACAACAAAA ATGGATTGGA 
     4201 TCTTCCACAC AATTAAACAG CATGCATTGA ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4261 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4321 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4381 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4441 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4501 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4561 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4621 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4681 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4741 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4801 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4861 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4921 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4981 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5041 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5101 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5161 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5221 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5281 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5341 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5401 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5461 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5521 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5581 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5641 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5701 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5761 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5821 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5881 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5941 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6001 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6061 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6481 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6961 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7021 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7081 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7141 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7201 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7261 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7321 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7501 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7561 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7621 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7681 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7741 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7801 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7861 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7921 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7981 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8041 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//