LOCUS       RC213039                7497 bp    DNA     circular  SYN 
DEFINITION  UBE3A (Myc-DDK-tagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 3
ACCESSION   NM_130839
VERSION     NM_130839.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3654..3745 
                     /label="Myc-DDK"  
     misc_feature    3775..4360 
                     /label="PolyA signal"  
     misc_feature    3832..3851 
                     /label="Restriction Enzyme"  
     misc_feature    4636..5308 
                     /label="ColE1"  
     misc_feature    5738..6532 
                     /label="Kan/Neo"  
     misc_feature    6567..6869 
                     /label="SV40 ori"  
     misc_feature    6955..7432 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3636..3642 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3636 
                     /label="UBE3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACAGCT TGTAAAAGAT CAGGAGAACC TCAGTCTGAC GACATTGAAG 
     1081 CTAGCCGAAT GAAGCGAGCA GCTGCAAAGC ATCTAATAGA ACGCTACTAC CACCAGTTAA 
     1141 CTGAGGGCTG TGGAAATGAA GCCTGCACGA ATGAGTTTTG TGCTTCCTGT CCAACTTTTC 
     1201 TTCGTATGGA TAATAATGCA GCAGCTATTA AAGCCCTCGA GCTTTATAAG ATTAATGCAA 
     1261 AACTCTGTGA TCCTCATCCC TCCAAGAAAG GAGCAAGCTC AGCTTACCTT GAGAACTCGA 
     1321 AAGGTGCCCC CAACAACTCC TGCTCTGAGA TAAAAATGAA CAAGAAAGGC GCTAGAATTG 
     1381 ATTTTAAAGA TGTGACTTAC TTAACAGAAG AGAAGGTATA TGAAATTCTT GAATTATGTA 
     1441 GAGAAAGAGA GGATTATTCC CCTTTAATCC GTGTTATTGG AAGAGTTTTT TCTAGTGCTG 
     1501 AGGCATTGGT ACAGAGCTTC CGGAAAGTTA AACAACACAC CAAGGAAGAA CTGAAATCTC 
     1561 TTCAAGCAAA AGATGAAGAC AAAGATGAAG ATGAAAAGGA AAAAGCTGCA TGTTCTGCTG 
     1621 CTGCTATGGA AGAAGACTCA GAAGCATCTT CCTCAAGGAT AGGTGATAGC TCACAGGGAG 
     1681 ACAACAATTT GCAAAAATTA GGCCCTGATG ATGTGTCTGT GGATATTGAT GCCATTAGAA 
     1741 GGGTCTACAC CAGATTGCTC TCTAATGAAA AAATTGAAAC TGCCTTTCTC AATGCACTTG 
     1801 TATATTTGTC ACCTAACGTG GAATGTGACT TGACGTATCA CAATGTATAC TCTCGAGATC 
     1861 CTAATTATCT GAATTTGTTC ATTATCGTAA TGGAGAATAG AAATCTCCAC AGTCCTGAAT 
     1921 ATCTGGAAAT GGCTTTGCCA TTATTTTGCA AAGCGATGAG CAAGCTACCC CTTGCAGCCC 
     1981 AAGGAAAACT GATCAGACTG TGGTCTAAAT ACAATGCAGA CCAGATTCGG AGAATGATGG 
     2041 AGACATTTCA GCAACTTATT ACTTATAAAG TCATAAGCAA TGAATTTAAC AGTCGAAATC 
     2101 TAGTGAATGA TGATGATGCC ATTGTTGCTG CTTCGAAGTG CTTGAAAATG GTTTACTATG 
     2161 CAAATGTAGT GGGAGGGGAA GTGGACACAA ATCACAATGA AGAAGATGAT GAAGAGCCCA 
     2221 TCCCTGAGTC CAGCGAGCTG ACACTTCAGG AACTTTTGGG AGAAGAAAGA AGAAACAAGA 
     2281 AAGGTCCTCG AGTGGACCCC CTGGAAACTG AACTTGGTGT TAAAACCCTG GATTGTCGAA 
     2341 AACCACTTAT CCCTTTTGAA GAGTTTATTA ATGAACCACT GAATGAGGTT CTAGAAATGG 
     2401 ATAAAGATTA TACTTTTTTC AAAGTAGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC 
     2461 CCTTTATATT GAATGCTGTC ACAAAGAATT TGGGATTATA TTATGACAAT AGAATTCGCA 
     2521 TGTACAGTGA ACGAAGAATC ACTGTTCTCT ACAGCTTAGT TCAAGGACAG CAGTTGAATC 
     2581 CATATTTGAG ACTCAAAGTT AGACGTGACC ATATCATAGA TGATGCACTT GTCCGGCTAG 
     2641 AGATGATCGC TATGGAAAAT CCTGCAGACT TGAAGAAGCA GTTGTATGTG GAATTTGAAG 
     2701 GAGAACAAGG AGTTGATGAG GGAGGTGTTT CCAAAGAATT TTTTCAGCTG GTTGTGGAGG 
     2761 AAATCTTCAA TCCAGATATT GGTATGTTCA CATACGATGA ATCTACAAAA TTGTTTTGGT 
     2821 TTAATCCATC TTCTTTTGAA ACTGAGGGTC AGTTTACTCT GATTGGCATA GTACTGGGTC 
     2881 TGGCTATTTA CAATAACTGT ATACTGGATG TACATTTTCC CATGGTTGTC TACAGGAAGC 
     2941 TAATGGGGAA AAAAGGAACT TTTCGTGACT TGGGAGACTC TCACCCAGTT CTATATCAGA 
     3001 GTTTAAAAGA TTTATTGGAG TATGAAGGGA ATGTGGAAGA TGACATGATG ATCACTTTCC 
     3061 AGATATCACA GACAGATCTT TTTGGTAACC CAATGATGTA TGATCTAAAG GAAAATGGTG 
     3121 ATAAAATTCC AATTACAAAT GAAAACAGGA AGGAATTTGT CAATCTTTAT TCTGACTACA 
     3181 TTCTCAATAA ATCAGTAGAA AAACAGTTCA AGGCTTTTCG GAGAGGTTTT CATATGGTGA 
     3241 CCAATGAATC TCCCTTAAAG TACTTATTCA GACCAGAAGA AATTGAATTG CTTATATGTG 
     3301 GAAGCCGGAA TCTAGATTTC CAAGCACTAG AAGAAACTAC AGAATATGAC GGTGGCTATA 
     3361 CCAGGGACTC TGTTCTGATT AGGGAGTTCT GGGAAATCGT TCATTCATTT ACAGATGAAC 
     3421 AGAAAAGACT CTTCTTGCAG TTTACAACGG GCACAGACAG AGCACCTGTG GGAGGACTAG 
     3481 GAAAATTAAA GATGATTATA GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC 
     3541 ATACTTGCTT TAATGTGCTT TTACTTCCGG AATACTCAAG CAAAGAAAAA CTTAAAGAGA 
     3601 GATTGTTGAA GGCCATCACG TATGCCAAAG GATTTGGCAT GCTGACGCGT ACGCGGCCGC 
     3661 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3721 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3781 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3841 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3901 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3961 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4021 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4081 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4141 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4201 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4261 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4321 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4381 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4441 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4501 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4561 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4621 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4681 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4741 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4801 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4861 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4921 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4981 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5041 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5161 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5221 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5281 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5341 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5401 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5461 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5521 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5581 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5641 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5701 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5761 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5821 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5881 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5941 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6001 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6061 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6121 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6181 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6241 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6301 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6361 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6421 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6481 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6541 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6601 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6661 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6721 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6781 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6901 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6961 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7021 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7081 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7141 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7201 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7261 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7321 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7381 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7441 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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