LOCUS       RC212987                8577 bp    DNA     circular  SYN 
DEFINITION  ABCB4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant C
ACCESSION   NM_018850
VERSION     NM_018850.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4734..4825 
                     /label="Myc-DDK"  
     misc_feature    4855..5440 
                     /label="PolyA signal"  
     misc_feature    4912..4931 
                     /label="Restriction Enzyme"  
     misc_feature    5716..6388 
                     /label="ColE1"  
     misc_feature    6818..7612 
                     /label="Kan/Neo"  
     misc_feature    7647..7949 
                     /label="SV40 ori"  
     misc_feature    8035..8512 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4716..4722 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4716 
                     /label="ABCB4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTTGAG GCGGCAAAGA ACGGAACAGC CTGGCGCCCC ACGAGCGCGG 
     1081 AGGGCGACTT TGAACTGGGC ATCAGCAGCA AACAAAAAAG GAAAAAAACG AAGACAGTGA 
     1141 AAATGATTGG AGTATTAACA TTGTTTCGAT ACTCCGATTG GCAGGATAAA TTGTTTATGT 
     1201 CGCTGGGTAC CATCATGGCC ATAGCTCACG GATCAGGTCT CCCCCTCATG ATGATAGTAT 
     1261 TTGGAGAGAT GACTGACAAA TTTGTTGATA CTGCAGGAAA CTTCTCCTTT CCAGTGAACT 
     1321 TTTCCTTGTC GCTGCTAAAT CCAGGCAAAA TTCTGGAAGA AGAAATGACT AGATATGCAT 
     1381 ATTACTACTC AGGATTGGGT GCTGGAGTTC TTGTTGCTGC CTATATACAA GTTTCATTTT 
     1441 GGACTTTGGC AGCTGGTCGA CAGATCAGGA AAATTAGGCA GAAGTTTTTT CATGCTATTC 
     1501 TACGACAGGA AATAGGATGG TTTGACATCA ACGACACCAC TGAACTCAAT ACGCGGCTAA 
     1561 CAGATGACAT CTCCAAAATC AGTGAAGGAA TTGGTGACAA GGTTGGAATG TTCTTTCAAG 
     1621 CAGTAGCCAC GTTTTTTGCA GGATTCATAG TGGGATTCAT CAGAGGATGG AAGCTCACCC 
     1681 TTGTGATAAT GGCCATCAGC CCTATTCTAG GACTCTCTGC AGCCGTTTGG GCAAAGATAC 
     1741 TCTCGGCATT TAGTGACAAA GAACTAGCTG CTTATGCAAA AGCAGGCGCC GTGGCAGAAG 
     1801 AGGCTCTGGG GGCCATCAGG ACTGTGATAG CTTTCGGGGG CCAGAACAAA GAGCTGGAAA 
     1861 GGTATCAGAA ACATTTAGAA AATGCCAAAG AGATTGGAAT TAAAAAAGCT ATTTCAGCAA 
     1921 ACATTTCCAT GGGTATTGCC TTCCTGTTAA TATATGCATC ATATGCACTG GCCTTCTGGT 
     1981 ATGGATCCAC TCTAGTCATA TCAAAAGAAT ATACTATTGG AAATGCAATG ACAGTTTTTT 
     2041 TTTCAATCCT AATTGGAGCT TTCAGTGTTG GCCAGGCTGC CCCATGTATT GATGCTTTTG 
     2101 CCAATGCAAG AGGAGCAGCA TATGTGATCT TTGATATTAT TGATAATAAT CCTAAAATTG 
     2161 ACAGTTTTTC AGAGAGAGGA CACAAACCAG ACAGCATCAA AGGGAATTTG GAGTTCAATG 
     2221 ATGTTCACTT TTCTTACCCT TCTCGAGCTA ACGTCAAGAT CTTGAAGGGC CTCAACCTGA 
     2281 AGGTGCAGAG TGGGCAGACG GTGGCCCTGG TTGGAAGTAG TGGCTGTGGG AAGAGCACAA 
     2341 CGGTCCAGCT GATACAGAGG CTCTATGACC CTGATGAGGG CACAATTAAC ATTGATGGGC 
     2401 AGGATATTAG GAACTTTAAT GTAAACTATC TGAGGGAAAT CATTGGTGTG GTGAGTCAGG 
     2461 AGCCGGTGCT GTTTTCCACC ACAATTGCTG AAAATATTTG TTATGGCCGT GGAAATGTAA 
     2521 CCATGGATGA GATAAAGAAA GCTGTCAAAG AGGCCAACGC CTATGAGTTT ATCATGAAAT 
     2581 TACCACAGAA ATTTGACACC CTGGTTGGAG AGAGAGGGGC CCAGCTGAGT GGTGGGCAGA 
     2641 AGCAGAGGAT CGCCATTGCA CGTGCCCTGG TTCGCAACCC CAAGATCCTT CTGCTGGATG 
     2701 AGGCCACGTC AGCATTGGAC ACAGAAAGTG AAGCTGAGGT ACAGGCAGCT CTGGATAAGG 
     2761 CCAGAGAAGG CCGGACCACC ATTGTGATAG CACACCGACT GTCTACGGTC CGAAATGCAG 
     2821 ATGTCATCGC TGGGTTTGAG GATGGAGTAA TTGTGGAGCA AGGAAGCCAC AGCGAACTGA 
     2881 TGAAGAAGGA AGGGGTGTAC TTCAAACTTG TCAACATGCA GACATCAGGA AGCCAGATCC 
     2941 AGTCAGAAGA ATTTGAACTA AATGATGAAA AGGCTGCCAC TAGAATGGCC CCAAATGGCT 
     3001 GGAAATCTCG CCTATTTAGG CATTCTACTC AGAAAAACCT TAAAAATTCA CAAATGTGTC 
     3061 AGAAGAGCCT TGATGTGGAA ACCGATGGAC TTGAAGCAAA TGTGCCACCA GTGTCCTTTC 
     3121 TGAAGGTCCT GAAACTGAAT AAAACAGAAT GGCCCTACTT TGTCGTGGGA ACAGTATGTG 
     3181 CCATTGCCAA TGGGGGGCTT CAGCCGGCAT TTTCAGTCAT ATTCTCAGAG ATCATAGCGA 
     3241 TTTTTGGACC AGGCGATGAT GCAGTGAAGC AGCAGAAGTG CAACATATTC TCTTTGATTT 
     3301 TCTTATTTCT GGGAATTATT TCTTTTTTTA CTTTCTTCCT TCAGGGTTTC ACGTTTGGGA 
     3361 AAGCTGGCGA GATCCTCACC AGAAGACTGC GGTCAATGGC TTTTAAAGCA ATGCTAAGAC 
     3421 AGGACATGAG CTGGTTTGAT GACCATAAAA ACAGTACTGG TGCACTTTCT ACAAGACTTG 
     3481 CCACAGATGC TGCCCAAGTC CAAGGAGCCA CAGGAACCAG GTTGGCTTTA ATTGCACAGA 
     3541 ATATAGCTAA CCTTGGAACT GGTATTATCA TATCATTTAT CTACGGTTGG CAGTTAACCC 
     3601 TATTGCTATT AGCAGTTGTT CCAATTATTG CTGTGTCAGG AATTGTTGAA ATGAAATTGT 
     3661 TGGCTGGAAA TGCCAAAAGA GATAAAAAAG AACTGGAAGC TGCTGGAAAG ATTGCAACAG 
     3721 AGGCAATAGA AAATATTAGG ACAGTTGTGT CTTTGACCCA GGAAAGAAAA TTTGAATCAA 
     3781 TGTATGTTGA AAAATTGTAT GGACCTTACA GGGTGTTTTC TGCAATTGTA TTTGGTGCAG 
     3841 TGGCTCTAGG ACATGCCAGT TCATTTGCTC CAGACTATGC TAAAGCTAAG CTGTCTGCAG 
     3901 CCCACTTATT CATGCTGTTT GAAAGACAAC CTCTGATTGA CAGCTACAGT GAAGAGGGGC 
     3961 TGAAGCCTGA TAAATTTGAA GGAAATATAA CATTTAATGA AGTCGTGTTC AACTATCCCA 
     4021 CCCGAGCAAA CGTGCCAGTG CTTCAGGGGC TGAGCCTGGA GGTGAAGAAA GGCCAGACAC 
     4081 TAGCCCTGGT GGGCAGCAGT GGCTGTGGGA AGAGCACGGT GGTCCAGCTC CTGGAGCGGT 
     4141 TCTACGACCC CTTGGCGGGG ACAGTGCTTC TCGATGGTCA AGAAGCAAAG AAACTCAATG 
     4201 TCCAGTGGCT CAGAGCTCAA CTCGGAATCG TGTCTCAGGA GCCTATCCTA TTTGACTGCA 
     4261 GCATTGCCGA GAATATTGCC TATGGAGACA ACAGCCGGGT TGTATCACAG GATGAAATTG 
     4321 TGAGTGCAGC CAAAGCTGCC AACATACATC CTTTCATCGA GACGTTACCC CACAAATATG 
     4381 AAACAAGAGT GGGAGATAAG GGGACTCAGC TCTCAGGAGG TCAAAAACAG AGGATTGCTA 
     4441 TTGCCCGAGC CCTCATCAGA CAACCTCAAA TCCTCCTGTT GGATGAAGCT ACATCAGCTC 
     4501 TGGATACTGA AAGTGAAAAG GTTGTCCAAG AAGCCCTGGA CAAAGCCAGA GAAGGCCGCA 
     4561 CCTGCATTGT GATTGCTCAC CGCCTGTCCA CCATCCAGAA TGCAGACTTA ATAGTGGTGT 
     4621 TTCAGAATGG GAGAGTCAAG GAGCATGGCA CGCATCAGCA GCTGCTGGCA CAGAAAGGCA 
     4681 TCTATTTTTC AATGGTCAGT GTCCAGGCTG GGACACAGAA CTTAACGCGT ACGCGGCCGC 
     4741 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4801 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4861 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4921 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4981 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5041 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5101 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5161 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5221 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5281 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5341 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5401 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5461 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5521 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5581 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5641 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5701 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5761 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5821 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5881 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5941 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6001 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6061 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6121 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6181 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6241 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6301 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6361 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6421 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6481 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6541 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6601 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6661 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6721 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6781 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6841 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6901 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6961 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7021 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7081 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7141 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7201 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7261 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7321 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7381 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7441 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7501 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7561 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7621 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7681 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7741 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7801 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7981 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8041 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8101 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8161 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8221 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8281 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8341 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8401 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8461 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8521 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//