LOCUS       RC212956                8694 bp    DNA     circular  SYN 
DEFINITION  DGKK (Myc-DDK-tagged)-Human diacylglycerol kinase, kappa (DGKK)
ACCESSION   NM_001013742
VERSION     NM_001013742.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4851..4942 
                     /label="Myc-DDK"  
     misc_feature    4972..5557 
                     /label="PolyA signal"  
     misc_feature    5029..5048 
                     /label="Restriction Enzyme"  
     misc_feature    5833..6505 
                     /label="ColE1"  
     misc_feature    6935..7729 
                     /label="Kan/Neo"  
     misc_feature    7764..8066 
                     /label="SV40 ori"  
     misc_feature    8152..8629 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4833..4839 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4833 
                     /label="DGKK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCGCGGA GCTGCCGCAG CCCAGGGCAC TGCCCCGCCT CAGGATGGAG 
     1081 AGCAGCCCGC TGAGTCTCCA GAGCCTCCGC CGCCTTGGCC GCCGCCGCCA CCACCACCGG 
     1141 CTCCGCCGCC GGCTCCGCCG CTGCTCTCCG AGGCTTCGCC AGAACCCATA CCAGAGCCCT 
     1201 GTCCAGAGCT TGCTCCAGGT CCCTGTCCAG AGGCGACCTC AGAATCAGCC ACAGAACTGT 
     1261 ACACAGAACC GACCCCAGAA CCAGCCACAG AGCCGGCCTC AGAACCGGCC CCAGAACCTG 
     1321 CCACAGAGCC GGCCCCAGAA CCTGCCACAG AGCCGGCCCC AGAACCGGCC CCAGAACCTG 
     1381 CCACAGAGTC TGCCCCAGAG CCGACTCCAG AACCTGCCCT AGAGTCGGTC CCAGAGCCGG 
     1441 CCCCAGAGCT GACTCCAGAA GTTGCCCCAG AGCTGGCCCC AGAGCCGACC CCAGAACCTG 
     1501 TGACAGAGCT GGCCCCAGAG TTCTGCCCTG AGGCGGCTCC AGAGTTCCGT CCAAGTCCAG 
     1561 CACCATGTCT ATTGCAATGT CCGGTGGACA CTCGAGAGAG AGGTCTAAAG ACCTCGCCAT 
     1621 CGCCATCGCC ATCGCCATCG CCCAGAACGC CAATGTCGTG GTCCAGAATA AAGAAAATAT 
     1681 TGAAGGAAGG ACCTATGCTG AAGAACTGTA ACTCTTTCAA GAGATGGAAG CTTAGATATT 
     1741 TTCTGGTTCA AGGACAGAAG CTCTACTTTG CACACCATCC CGCGTTTGCA CACTTTGAAA 
     1801 CGATTGATCT GTCTCAAGCC ACTGTGGCAG AAAGCAGCTG TAGAAACCTT TGCCACAGTT 
     1861 TTTGTGTTAT TACACCACAA CGAAAAATCA CTCTGGCTGC ACCCAACCGG AAAGACATGG 
     1921 AAGAATGGAT TAACATCATA AAAACCATCC AACAGGGAGA AATTTATAAG ATACCTGCAG 
     1981 CAGAAAACAA CCCTTTTCTT GTTGGAATGC ATTGTTGGTA CTCCAGTTAC AGCCACCGGA 
     2041 CCCAGCACTG CAATGTTTGT CGAGAGAGCA TTCCTGCCTT ATCTAGAGAT GCCATCATCT 
     2101 GTGAAGTGTG CAAAGTGAAA TCTCACAGAT TGTGTGCTTT GAGAGCAAGC AAAGACTGCA 
     2161 AGTGGAATAC ATTGTCTATC ACTGATGACC TCCTTCTGCC TGCAGATGAA GTAAACATGC 
     2221 CCCATCAATG GGTAGAAGGA AACATGCCTG TCAGCTCTCA GTGTGCAGTG TGTCATGAGA 
     2281 GCTGTGGCAG TTATCAAAGA CTTCAAGACT TCCGCTGCCT GTGGTGTAAT TCTACGGTGC 
     2341 ATGATGACTG TAGGAGACGG TTTTCCAAGG AATGTTGCTT CAGAAGCCAT CGCTCATCAG 
     2401 TCATTCCTCC CACTGCTCTA AGCGACCCCA AAGGCGATGG CCAATTAGTA GTATCTTCAG 
     2461 ACTTCTGGAA TCTTGATTGG TCATCAGCCT GTTCATGTCC CTTGCTCATC TTCATCAACT 
     2521 CCAAAAGTGG CGATCATCAG GGGATCGTCT TCCTCCGAAA ATTCAAGCAA TACCTTAACC 
     2581 CATCTCAAGT GTTCGACTTA TTGAAGGGTG GACCTGAAGC AGGGCTGTCT ATGTTCAAGA 
     2641 ACTTTGCTCG CTTTCGCATT CTGGTTTGTG GTGGAGATGG CAGCGTGAGC TGGGTCTTAT 
     2701 CTCTGATTGA TGCCTTTGGA TTACATGAAA AGTGTCAGTT GGCAGTCATC CCACTTGGAA 
     2761 CCGGCAATGA TCTGGCTCGT GTTCTGGGCT GGGGTGCATT CTGGAACAAA AGCAAGTCAC 
     2821 CTCTGGACAT CCTCAACAGA GTGGAGCAGG CTAGTGTGAG GATCCTAGAC AGATGGAGTG 
     2881 TGATGATTCG TGAGACTCCC AGACAAACCC CGCTGCTAAA AGGACAGGTT GAAATGGATG 
     2941 TACCACGATT TGAGGCTGCT GCCATCCAAC ACTTAGAATC TGCAGCCACC GAGTTGAACA 
     3001 AAATCCTGAA GGCCAAGTAC CCCACAGAGA TGATCATCGC AACCAGATTC TTGTGTTCAG 
     3061 CTGTGGAAGA TTTTGTGGTT GATATTGTAA AGGCCTGGGG TCAGATAAAA CAGAACAACA 
     3121 CTGCAATAGT GTCTGTGATT TTGAAAAGTG ACTTAATGTA TGATAGGCTC AGTGTCCTGA 
     3181 TCGATGTCCT GGCTGAGGAG GCAGCAGCTA CTTCTGCTGA AAAAAGTGCT ACAGAATATG 
     3241 CAGACAGCAG CAAGGCAGAT AGGAAGCCCT TCATTCCTCA AATAGACCAC ATAGCCAAGT 
     3301 GCAAGTTGGA GCTGGCTACA AAGGCCCAGA GTCTCCAGAA ATCCTTGAAA CTCATCATAT 
     3361 TTCAAGTTGA ACAAGCTCTG GATGAGGAAA GCAGACAGAC AATATCTGTT AAGAACTTTA 
     3421 GTTCAACTTT CTTCCTGGAA GATGACCCAG AAGATATTAA CCAGACAAGC CCACGACGCC 
     3481 GTTCTCGTCG TGGCACTTTG TCTTCTATAT CTTCTCTCAA AAGTGAGGAC CTGGACAACC 
     3541 TTAACTTGGA TCACTTACAT TTTACACCTG AATCTATACG CTTCAAAGAA AAATGTGTCA 
     3601 TGAACAACTA CTTCGGAATT GGACTGGATG CTAAAATTTC TCTGGACTTC AACACCAGAA 
     3661 GAGATGAACA CCCAGGGCAA TACAATAGCC GCCTTAAGAA CAAGATGTGG TATGGCCTTC 
     3721 TGGGAACCAA AGAACTTTTG CAGCGCTCTT ACAGGAAACT GGAAGAACGA GTGCATTTGG 
     3781 AGTGTGATGG AGAAACCATC TCCTTGCCAA ACCTGCAAGG CATTGTAGTG CTCAACATTA 
     3841 CCAGCTATGC TGGAGGTATC AACTTCTGGG GAAGCAACAC AGCAACCACG GAATATGAGG 
     3901 CTCCTGCAAT CGATGATGGG AAACTGGAGG TGGTGGCAAT CTTTGGTTCT GTGCAGATGG 
     3961 CAATGTCCCG TATCATCAAC CTGCATCATC ATCGCATTGC CCAGTGCCAT GAGGTGATGA 
     4021 TAACCATTGA TGGTGAAGAA GGTATCCCAG TGCAGGTGGA TGGGGAGGCC TGGATTCAGA 
     4081 GACCAGGCCT TATCAAAATT AGATACAAGA ACGCTGCCCA GATGCTGACA AGAGATCGGG 
     4141 ACTTTGAGAA CTCAATGAAA ATGTGGGAAT ACAAGCATAC TGAAATTCAA GCTGCCCCTC 
     4201 AACCCCAGCT GGACTTCCAG GACTCTCAAG AGAGCCTCTC TGACGAGGAG TATGCCCAGA 
     4261 TGCAGCACTT AGCTCGGCTT GCAGAAAACC TCATCAGCAA ACTTAATGAC CTGAGCAAGA 
     4321 TCCACCAGCA TGTGTCTGTC CTCATGGGTT CTGTGAATGC CAGCGCTAAC ATCCTGAATG 
     4381 ATATATTTTA CGGCCAAGAC AGTGGCAATG AGATGGGTGC AGCTTCCTGT ATTCCCATTG 
     4441 AAACTCTAAG CAGAAATGAT GCCGTAGATG TTACATTTAG TCTTAAAGGT CTCTACGATG 
     4501 ACACCACAGC TTTCCTGGAT GAAAAGCTGC TGAGAAGTGC TGAGGATGAG ACTGCACTAC 
     4561 AAAGCGCCCT GGATGCCATG AATAAGGAGT TCAAAAAGCT ATCTGAGATT GACTGGATGA 
     4621 ATCCAATCTT TGTTCCAGAG GAAAAATCTT CGGACACTGA CAGTAGAAGC CTCAGGCTGA 
     4681 AAATTAAGTT CCCCAAATTG GGAAAGAAAA AGGTAGAAGA GGAACGCAAG CCTAAATCAG 
     4741 GCCAGAGTGT CCAGAGTTTT ATTGGCAATT TATGGCACCG CAGACATCGT GAAGATGAAG 
     4801 CAGAGGGTGA TGATCCTCTA ACACCATCGA GATCTCAACT GACGCGTACG CGGCCGCTCG 
     4861 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4921 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4981 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5041 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5101 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5161 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5221 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5281 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5341 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5401 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5461 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5521 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5581 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5641 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5701 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5761 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5821 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5881 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5941 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6001 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6061 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6121 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6181 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6241 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6301 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6361 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6421 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6481 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6541 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6601 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6661 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6721 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6781 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6841 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6901 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6961 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7021 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7081 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7141 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7201 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7261 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7321 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7381 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7441 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7501 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7561 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7621 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7681 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7741 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7801 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7861 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7921 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8101 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8161 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8221 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//