LOCUS       RC212932L1                8991 bp    DNA     circular  SYN 
DEFINITION  KCNQ2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 1
ACCESSION   NM_172107
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7282..7462 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8190..8849 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7621..8189 
                     /label="ColE1"  
     misc_feature    6157..6177 
                     /label="LR50 primer"  
     misc_feature    5954..6025 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6166..6757 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5936..5942 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5936 
                     /label="KCNQ2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGTGCAGAAG TCGCGCAACG GCGGCGTATA 
     3361 CCCCGGCCCG AGCGGGGAGA AGAAGCTGAA GGTGGGCTTC GTGGGGCTGG ACCCCGGCGC 
     3421 GCCCGACTCC ACCCGGGACG GGGCGCTGCT GATCGCCGGC TCCGAGGCCC CCAAGCGCGG 
     3481 CAGCATCCTC AGCAAACCTC GCGCGGGCGG CGCGGGCGCC GGGAAGCCCC CCAAGCGCAA 
     3541 CGCCTTCTAC CGCAAGCTGC AGAATTTCCT CTACAACGTG CTGGAGCGGC CGCGCGGCTG 
     3601 GGCGTTCATC TACCACGCCT ACGTGTTCCT CCTGGTTTTC TCCTGCCTCG TGCTGTCTGT 
     3661 GTTTTCCACC ATCAAGGAGT ATGAGAAGAG CTCGGAGGGG GCCCTCTACA TCCTGGAAAT 
     3721 CGTGACTATC GTGGTGTTTG GCGTGGAGTA CTTCGTGCGG ATCTGGGCCG CAGGCTGCTG 
     3781 CTGCCGGTAC CGTGGCTGGA GGGGGCGGCT CAAGTTTGCC CGGAAACCGT TCTGTGTGAT 
     3841 TGACATCATG GTGCTCATCG CCTCCATTGC GGTGCTGGCC GCCGGCTCCC AGGGCAACGT 
     3901 CTTTGCCACA TCTGCGCTCC GGAGCCTGCG CTTCCTGCAG ATTCTGCGGA TGATCCGCAT 
     3961 GGACCGGCGG GGAGGCACCT GGAAGCTGCT GGGCTCTGTG GTCTATGCCC ACAGCAAGGA 
     4021 GCTGGTCACT GCCTGGTACA TCGGCTTCCT TTGTCTCATC CTGGCCTCGT TCCTGGTGTA 
     4081 CTTGGCAGAG AAGGGGGAGA ACGACCACTT TGACACCTAC GCGGATGCAC TCTGGTGGGG 
     4141 CCTGATCACG CTGACCACCA TTGGCTACGG GGACAAGTAC CCCCAGACCT GGAACGGCAG 
     4201 GCTCCTTGCG GCAACCTTCA CCCTCATCGG TGTCTCCTTC TTCGCGCTGC CTGCAGGCAT 
     4261 CTTGGGGTCT GGGTTTGCCC TGAAGGTTCA GGAGCAGCAC AGGCAGAAGC ACTTTGAGAA 
     4321 GAGGCGGAAC CCGGCAGCAG GCCTGATCCA GTCGGCCTGG AGATTCTACG CCACCAACCT 
     4381 CTCGCGCACA GACCTGCACT CCACGTGGCA GTACTACGAG CGAACGGTCA CCGTGCCCAT 
     4441 GTACAGTTCG CAAACTCAAA CCTACGGGGC CTCCAGACTT ATCCCCCCGC TGAACCAGCT 
     4501 GGAGCTGCTG AGGAACCTCA AGAGTAAATC TGGACTCGCT TTCAGGAAGG ACCCCCCGCC 
     4561 GGAGCCGTCT CCAAGTAAAG GCAGCCCGTG CAGAGGGCCC CTGTGTGGAT GCTGCCCCGG 
     4621 ACGCTCTAGC CAGAAGGTCA GTTTGAAAGA TCGTGTCTTC TCCAGCCCCC GAGGCGTGGC 
     4681 TGCCAAGGGG AAGGGGTCCC CGCAGGCCCA GACTGTGAGG CGCTCACCCA GCGCCGACCA 
     4741 GAGCCTCGAG GACAGCCCCA GCAAGGTGCC CAAGAGCTGG AGCTTCGGGG ACCGCAGCCG 
     4801 GGCACGCCAG GCTTTCCGCA TCAAGGGTGC CGCGTCACGG CAGAACTCAG AAGAAGCAAG 
     4861 CCTCCCCGGA GAGGACATTG TGGATGACAA GAGCTGCCCC TGCGAGTTTG TGACCGAGGA 
     4921 CCTGACCCCG GGCCTCAAAG TCAGCATCAG AGCCGTGTGT GTCATGCGGT TCCTGGTGTC 
     4981 CAAGCGGAAG TTCAAGGAGA GCCTGCGGCC CTACGACGTG ATGGACGTCA TCGAGCAGTA 
     5041 CTCAGCCGGC CACCTGGACA TGCTGTCCCG AATTAAGAGC CTGCAGTCCA GAGTGGACCA 
     5101 GATCGTGGGG CGGGGCCCAG CGATCACGGA CAAGGACCGC ACCAAGGGCC CGGCCGAGGC 
     5161 GGAGCTGCCC GAGGACCCCA GCATGATGGG ACGGCTCGGG AAGGTGGAGA AGCAGGTCTT 
     5221 GTCCATGGAG AAGAAGCTGG ACTTCCTGGT GAATATCTAC ATGCAGCGGA TGGGCATCCC 
     5281 CCCGACAGAG ACCGAGGCCT ACTTTGGGGC CAAAGAGCCG GAGCCGGCGC CGCCGTACCA 
     5341 CAGCCCGGAA GACAGCCGGG AGCATGTCGA CAGGCACGGC TGCATTGTCA AGATCGTGCG 
     5401 CTCCAGCAGC TCCACGGGCC AGAAGAACTT CTCGGCGCCC CCGGCCGCGC CCCCTGTCCA 
     5461 GTGTCCGCCC TCCACCTCCT GGCAGCCACA GAGCCACCCG CGCCAGGGCC ACGGCACCTC 
     5521 CCCCGTGGGG GACCACGGCT CCCTGGTGCG CATCCCGCCG CCGCCTGCCC ACGAGCGGTC 
     5581 GCTGTCCGCC TACGGCGGGG GCAACCGCGC CAGCATGGAG TTCCTGCGGC AGGAGGACAC 
     5641 CCCGGGCTGC AGGCCCCCCG AGGGGAACCT GCGGGACAGC GACACGTCCA TCTCCATCCC 
     5701 GTCCGTGGAC CACGAGGAGC TGGAGCGTTC CTTCAGCGGC TTCAGCATCT CCCAGTCCAA 
     5761 GGAGAACCTG GATGCTCTCA ACAGCTGCTA CGCGGCCGTG GCGCCTTGTG CCAAAGTCAG 
     5821 GCCCTACATT GCGGAGGGAG AGTCAGACAC CGACTCCGAC CTCTGTACCC CGTGCGGGCC 
     5881 CCCGCCACGC TCGGCCACCG GCGAGGGTCC CTTTGGTGAC GTGGGCTGGG CCGGGCCCAG 
     5941 GAAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6001 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6061 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6121 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6181 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6241 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6301 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6361 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6421 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6481 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6541 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6601 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6661 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6721 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     6781 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     6841 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     6901 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     6961 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7021 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7081 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7141 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7201 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7261 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7321 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7381 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7441 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7501 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7561 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7621 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7681 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7741 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     7801 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     7861 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     7921 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     7981 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8041 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8101 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8161 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8221 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8281 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8341 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8401 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8461 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8521 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8581 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8641 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8701 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     8761 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     8821 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     8881 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8941 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//