LOCUS       RC212893                8436 bp    DNA     circular  SYN 
DEFINITION  PPFIA1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 1
ACCESSION   NM_177423
VERSION     NM_177423.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4593..4684 
                     /label="Myc-DDK"  
     misc_feature    4714..5299 
                     /label="PolyA signal"  
     misc_feature    4771..4790 
                     /label="Restriction Enzyme"  
     misc_feature    5575..6247 
                     /label="ColE1"  
     misc_feature    6677..7471 
                     /label="Kan/Neo"  
     misc_feature    7506..7808 
                     /label="SV40 ori"  
     misc_feature    7894..8371 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4575..4581 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4575 
                     /label="PPFIA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGCGAG GTGATGCCGA CCATCAGCGA AGCAGAAGGC CCCCCTGGAG 
     1081 GAGGTGGAGG CCATGGTTCC GGCTCCCCTT CACAGCCAGA TGCAGATTCA CATTTTGAAC 
     1141 AGTTGATGGT CTCCATGCTA GAAGAAAGGG ACCGCCTTCT TGATACACTG AGAGAGACTC 
     1201 AAGAAACGCT GGCCTTAACC CAGGGGAAGT TACACGAGGT TGGTCATGAA AGAGATTCCT 
     1261 TGCAGAGACA GCTCAACACG GCACTTCCAC AGGAGTTCGC AGCACTTACT AAAGAACTCA 
     1321 ATGTATGCAG GGAACAGCTC CTTGAAAGGG AAGAAGAAAT TGCTGAACTG AAAGCAGAAA 
     1381 GGAATAACAC CAGGCTGCTG TTAGAGCATT TGGAATGCCT TGTCTCCAGG CATGAGCGGT 
     1441 CTCTTAGGAT GACCGTGGTG AAGAGACAAG CGCAGTCTCC AGCAGGCGTG TCCAGCGAAG 
     1501 TGGAAGTGCT GAAAGCACTG AAGTCCTTAT TTGAACACCA CAAAGCTCTG GATGAAAAGG 
     1561 TGAGAGAGCG ATTACGAGTA GCACTTGAAA GATGTAGTTT GTTAGAAGAG GAATTAGGTG 
     1621 CCACACACAA AGAGCTAATG ATTCTTAAAG AACAGAATAA TCAGAAAAAA ACTCTAACAG 
     1681 ATGGAGTGCT GGACATAAAC CATGAACAAG AAAATACACC AAGCACGAGT GGAAAGAGAT 
     1741 CTTCTGATGG TTCTTTAAGC CACGAGGAAG ACCTTGCTAA AGTAATTGAG CTCCAAGAAA 
     1801 TCATAAGTAA GCAGTCAAGG GAACAGAGCC AAATGAAAGA ACGCCTGGCT TCCCTTTCCA 
     1861 GTCATGTGAC AGAACTGGAA GAGGATCTGG ACACGGCTAG AAAAGATCTC ATCAAATCTG 
     1921 AAGAAATGAA CACAAAATTG CAACGAGATG TCCGTGAAGC CATGGCCCAA AAGGAAGATA 
     1981 TGGAAGAGAG AATCACTACT CTTGAAAAAC GCTACCTCGC TGCACAGCGT GAAGCCACAT 
     2041 CTGTGCATGA CCTCAATGAT AAACTTGAAA ATGAAATTGC AAATAAAGAT TCTATGCATC 
     2101 GACAGACTGA AGATAAAAAC CGCCAGTTAC AGGAGCGCTT GGAATTGGCA GAGCAAAAGC 
     2161 TGCAACAGAC ACTGAGGAAG GCAGAGACGC TCCCGGAGGT GGAGGCGGAG CTGGCCCAGA 
     2221 GGGTGGCAGC GCTTTCCAAG GCTGAAGAGA GACACGGCAA CATTGAAGAA AGGTTACGAC 
     2281 AGATGGAAGC ACAGTTGGAG GAGAAGAATC AAGAACTGCA GCGGGCAAGG CAAAGAGAAA 
     2341 AAATGAACGA AGAACATAAT AAACGTTTAT CAGACACTGT TGACAAGCTG CTTTCAGAAT 
     2401 CTAATGAGAG GCTTCAACTT CATCTTAAAG AGAGAATGGC TGCTTTGGAA GATAAGAACT 
     2461 CTCTTTTAAG AGAAGTTGAA AGTGCAAAAA AGCAGTTAGA AGAAACACAA CACGATAAGG 
     2521 ATCAGCTTGT CCTAAACATT GAAGCACTGA GGGCTGAACT AGACCACATG AGACTAAGAG 
     2581 GTGCTTCACT TCATCATGGC CGACCCCACT TGGGCAGTGT CCCAGATTTC AGGTTCCCCA 
     2641 TGGCAGACGG CCACACAGAC TCCTACAGCA CCAGTGCAGT GCTGCGGCGC CCACAGAAAG 
     2701 GCCGGCTGGC AGCCCTGCGA GATGAGCCTT CCAAGGTACA AACTCTTAAT GAGCAGGATT 
     2761 GGGAACGTGC CCAGCAAGCT AGTGTCTTGG CAAATGTAGC ACAAGCATTC GAGAGTGATG 
     2821 CTGACGTGTC TGATGGTGAA GATGACAGGG ACACTCTCCT CAGCTCAGTT GACCTGCTAT 
     2881 CGCCCAGCGG GCAGGCCGAC GCGCACACAC TAGCCATGAT GCTTCAGGAG CAGCTGGACG 
     2941 CCATCAACAA AGAGATCAGG TTGATTCAGG AAGAAAAAGA AAATACAGAG CAGCGGGCAG 
     3001 AGGAGATTGA AAGTCGAGTT GGCAGTGGAA GTCTAGACAA TCTTGGTCGT TTTAGATCAA 
     3061 TGAGCTCCAT TCCCCCCTAC CCTGCTTCCT CGCTTGCTAG CTCCTCCCCT CCGGGCAGTG 
     3121 GGCGCTCCAC CCCACGAAGG ATCCCTCACA GCCCAGCTCG GGAAGTGGAC AGACTGGGCG 
     3181 TCATGACCCT TTTGCCACCT TCCAGAGAAG AGGTACGAGA TGACAAGACA ACCATAAAGT 
     3241 GTGAAACCTC CCCGCCTTCC TCCCCGAGAG CCCTTCGGTT AGACCGGCTG CACAAAGGGG 
     3301 CGCTGCACAC CGTCAGCCAC GAGGACATCA GGGACATAAG GAACTCCACA GGCTCCCAGG 
     3361 ATGGTCCCGT GAGCAACCCC AGCAGTAGCA ACAGTAGCCA GGACTCGCTC CACAAAGCCC 
     3421 CAAAGAAGAA AGGCATTAAG TCCTCCATTG GCCGCTTGTT TGGCAAGAAA GAAAAGGGCC 
     3481 GACCTGGACA AACTGGCAAA GAAGCATTAG GACAAGCTGG TGTTTCCGAG ACGGATAACT 
     3541 CATCTCAGGA TGCCTTGGGA CTTAGCAAAT TGGGGGGACA GGCTGAAAAA AATCGTAAAC 
     3601 TTCAAAAAAA GCATGAATTG CTGGAGGAAG CCCGGAGACA AGGTTTACCT TTTGCCCAAT 
     3661 GGGACGGGCC AACGGTTGTG GTCTGGCTAG AGCTCTGGGT TGGGATGCCA GCCTGGTATG 
     3721 TGGCTGCCTG CCGAGCAAAC GTGAAAAGCG GGGCCATCAT GTCGGCCCTG TCCGACACAG 
     3781 AGATCCAGCG TGAGATTGGC ATCAGCAACC CCCTGCACAG GCTGAAGCTG AGGCTGGCCA 
     3841 TCCAGGAGAT CATGTCGCTG ACCAGCCCGT CTGCCCCGCC CACATCTAGA ACGACACTCG 
     3901 CCTATGGGGA CATGAACCAC GAGTGGATCG GCAACGAGTG GCTCCCCAGC CTGGGCCTCC 
     3961 CCCAGTACCG CAGCTACTTC ATGGAGTGCC TTGTAGACGC CAGGATGCTG GACCACTTGA 
     4021 CCAAGAAAGA CCTTCGAGGG CAGCTGAAAA TGGTCGACAG TTTTCACAGA AACAGTTTCC 
     4081 AGTGTGGAAT TATGTGCCTG AGAAGGTTAA ATTATGACCG GAAAGAACTG GAAAGAAAAA 
     4141 GAGAAGAAAG TCAGAGTGAA ATAAAAGACG TGCTTGTTTG GAGCAATGAT CGAGTGATTC 
     4201 GCTGGATCCT GTCAATTGGC CTTAAAGAAT ATGCAAACAA TCTTATAGAG AGTGGTGTTC 
     4261 ACGGAGCACT TCTGGCCTTA GATGAAACCT TCGACTTCAG TGCACTGGCA CTGCTGTTAC 
     4321 AGATCCCGAC GCAGAACACA CAGGCTCGTG CTGTCTTGGA AAGAGAATTT AACAACCTTT 
     4381 TGGTCATGGG GACTGATAGA AGGTTTGATG AAGATGATGA TAAAAGCTTT AGGAGAGCAC 
     4441 CTTCATGGAG AAAAAAGTTT AGACCAAAGG ACATTCGTGG CTTAGCTGCT GGGTCAGCAG 
     4501 AGACTCTCCC TGCAAACTTC CGGGTGACTT CTTCTATGTC TTCCCCCTCT ATGCAGCCAA 
     4561 AGAAGATGCA GATGGACGGT ATGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4621 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4681 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4741 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4801 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4861 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4921 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4981 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5041 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5101 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5161 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5221 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5281 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5341 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5401 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5461 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5521 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5581 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5641 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5701 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5761 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5821 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5881 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5941 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6001 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6061 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6121 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6181 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6241 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6301 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6361 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6421 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6481 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6541 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6601 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6661 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6721 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6781 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6841 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6901 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6961 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7021 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7081 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7141 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7201 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7261 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7321 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7381 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7441 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7501 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7561 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7621 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7681 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7801 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7861 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7921 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7981 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8041 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8101 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8161 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8221 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8281 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8341 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8401 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//