LOCUS       RC212877                9090 bp    DNA     circular  SYN 
DEFINITION  EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5
ACCESSION   NM_004953
VERSION     NM_004953.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="EIF4G1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG 
     1081 GTCTGGAGCC TCAAGCTAAT GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG 
     1141 ACCGGTCACA GGGAGCAATC ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC 
     1201 CTTCAGAATC CCAGCCTTCG TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC 
     1261 CGGGGTCTGA GCCTAATCTC GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC 
     1321 AAATGTCTGT AGAAGAATCA ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT 
     1381 CTCCAGAACC CACTCCTCTC GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC 
     1441 CGGTTCCAGA ATCTGAGTTT TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC 
     1501 ACACAGTGGA AATTCATGAG CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG 
     1561 TGGAGTCAAG CCCAGAGCTT GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA 
     1621 TTGCTCCAAC TGCCCAACCT GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG 
     1681 ACTTAAGCCC AGTCAGTGAG CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAGTGGCGC 
     1741 CCCCCACCAT CCCCTCTGCT ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG 
     1801 AGGAGGAAAT GGAAGAAGAA GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG 
     1861 CTGAGAGTGA GAAAGGAGGA GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA 
     1921 ACTTGTCTCA GAATTTGGAG GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA 
     1981 GGAGACGGAA AATTAAGGAG CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT 
     2041 TCAAGGAGGC GAACCCGGCA GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC 
     2101 CAGGCCCAGA GTCTGAGGGC AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT 
     2161 GGGACTCAAA GGAAGACAAA ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT 
     2221 ATGAATATAA GTCAGATCAG TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC 
     2281 GTGAGTTCCT GCTTGGTTTT CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC 
     2341 CACATATCAG TGACGTGGTG CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC 
     2401 CCACTAGACT ACAAGGCATA AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG 
     2461 GCCGGACAAC CCTTAGCACC CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC 
     2521 GTGGGCCGGC TGGCCTGGGA CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA 
     2581 AGATCATTGC CACAGTGTTA ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT 
     2641 GGAAACCCAG CAGCAAGCGG ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG 
     2701 GCAGCAAAAC CCAGGACCTA TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC 
     2761 AGATGTTCCA GCAGCTGATG AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC 
     2821 TCAAAGGGGT CATTGACCTC ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG 
     2881 CCTATGCCAA CATGTGCCGC TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA 
     2941 CAGTGACTGT GAACTTCCGA AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG 
     3001 ACAAAGATGA TGATGAGGTT TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG 
     3061 CAGAGGAACG AGGACGCCTG AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC 
     3121 GCTCTTTAGG GAATATCAAG TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG 
     3181 CAATAATGCA TGACTGTGTG GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT 
     3241 GCCTTTGTCG TCTGCTCACC ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC 
     3301 GAATGGATCA GTATTTCAAC CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC 
     3361 GCATCCGCTT TATGCTGCAG GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC 
     3421 GAGGGGATCA GGGTCCCAAG ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC 
     3481 ATCGAGAGCA CATCAAAGTG CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG 
     3541 GTCCTCCAGG CCCTCCCATC AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA 
     3601 CAGTTCCCAT CAGCAAAGGT AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA 
     3661 AGCCTGGCTC CATCGATTCT AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG 
     3721 GCAAGGGCAG CAGCGGAGGC TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC 
     3781 GCCCAGCTAC TAGTACTTTG AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA 
     3841 GCACAGATAA TAGACGTGTG GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG 
     3901 CTGGAGACCG AGGAGACCGC CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC 
     3961 TTGATCGTGC GCGGACACCT GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA 
     4021 GTAGAGAACG GCCCTCCCAG CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC 
     4081 GGGACCGTGG GCGGGATGCC GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA 
     4141 AGGCGGCTCT CTCTGAGGAG GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC 
     4201 TCCATCTCAA TGACATGAAA GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT 
     4261 TGCTCTTCAT CTTTGTACGG CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC 
     4321 GTGAGCATAT GGGGCAGCTG CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC 
     4381 AGTACTACCA AGGGTTGTAT GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC 
     4441 CCCACGTGTG GCTCTACCTA GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC 
     4501 CCATGGGGGA GCTGTTCAGG GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT 
     4561 CCCTGTTGCT GGAGATCCTG GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA 
     4621 CGCTGTGGCG AGAAGCCGGG CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG 
     4681 GTGCATTCGT CGCTGAACAG AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG 
     4741 GCCAGAGGGC ACTCCCCTCC GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG 
     4801 GCAGCAGTAA CCAGCGGGTG TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG 
     4861 TATCCAACAC GTTAGTTCGA GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG 
     4921 AGACTCCCCT CCGAGTGGAC GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT 
     4981 ACCTGTGTGA CGAGCAGAAG GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA 
     5041 CCTTAGAACA GCCTCCCAAC CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG 
     5101 TGGTGAAGGA GGATGCCTTC TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG 
     5161 GCAAGGGTGT GGCCCTTAAA TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG 
     5221 AGGAGTCTGA CCACAACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//