LOCUS       RC212873L2                7876 bp    DNA     circular  SYN 
DEFINITION  ALKBH3 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 3 (E. coli) (ALKBH3)
ACCESSION   NM_139178
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6167..6347 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7075..7734 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6395..7014 
                     /label="ColE1"  
     misc_feature    5042..5062 
                     /label="LR50"  
     misc_feature    4182..4907 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5051..5642 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4161..4167 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4161 
                     /label="ALKBH3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGGAAAAA AGACGGCGAG CCCGAGTTCA 
     3361 GGGAGCCTGG GCTGCCCCTG TTAAAAGCCA GGCCATTGCT CAGCCAGCTA CCACTGCTAA 
     3421 GAGCCATCTC CACCAGAAGC CTGGCCAGAC CTGGAAGAAC AAAGAGCATC ATCTCTCTGA 
     3481 CAGAGAGTTT GTGTTCAAAG AACCTCAGCA GGTAGTACGT AGAGCTCCTG AGCCACGAGT 
     3541 GATTGACAGA GAGGGTGTGT ATGAAATCAG CCTGTCACCC ACAGGTGTAT CTAGGGTCTG 
     3601 TTTGTATCCT GGCTTTGTTG ACGTGAAAGA AGCTGACTGG ATATTGGAAC AGCTTTGTCA 
     3661 AGATGTTCCC TGGAAACAGA GGACCGGCAT CAAAGAGGAT ATAACTTATC AGCAACCAAG 
     3721 ACTTACAGCA TGGTATGGAG AACTTCCTTA CACTTATTCA AGAATCACTA TGGAACCAAA 
     3781 TCCTCACTGG CACCCTGTGC TGCGCACACT AAAGAACCGC ATTGAAGAGA ACACTGGCCA 
     3841 CACCTTCAAC TCCTTACTCT GCAATCTTTA TCGCAATGAG AAGGACAGCG TGGACTGGCA 
     3901 CAGTGATGAT GAACCCTCAC TAGGGAGGTG CCCCATTATT GCTTCACTAA GTTTTGGTGC 
     3961 CACACGCACA TTTGAGATGA GAAAGAAGCC ACCACCAGAA GAGAATGGAG AGTACACATA 
     4021 TGTGGAAAGA GTGAAGATAC CCTTGGATCA TGGGACCTTG TTAATCATGG AAGGAGCGAC 
     4081 ACAAGCTGAC TGGCAGCATC GAGTGCCCAA AGAATACCAC TCTAGAGAAC CGAGAGTGAA 
     4141 CCTGACCTTT CGGACAGTCT ATCCAGACCA CGCGTACGCG GCCGCTCGAG ATGAGCGGGG 
     4201 GCGAGGAGCT GTTCGCCGGC ATCGTGCCCG TGCTGATCGA GCTGGACGGC GACGTGCACG 
     4261 GCCACAAGTT CAGCGTGCGC GGCGAGGGCG AGGGCGACGC CGACTACGGC AAGCTGGAGA 
     4321 TCAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC 
     4381 TCTGCTACGG CATCCAGTGC TTCGCCCGCT ACCCCGAGCA CATGAAGATG AACGACTTCT 
     4441 TCAAGAGCGC CATGCCCGAG GGCTACATCC AGGAGCGCAC CATCCAGTTC CAGGACGACG 
     4501 GCAAGTACAA GACCCGCGGC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 
     4561 AGCTGAAGGG CAAGGACTTC AAGGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA 
     4621 GCTTCAACAG CCACAACGTG TACATCCGCC CCGACAAGGC CAACAACGGC CTGGAGGCTA 
     4681 ACTTCAAGAC CCGCCACAAC ATCGAGGGCG GCGGCGTGCA GCTGGCCGAC CACTACCAGA 
     4741 CCAACGTGCC CCTGGGCGAC GGCCCCGTGC TGATCCCCAT CAACCACTAC CTGAGCACTC 
     4801 AGACCAAGAT CAGCAAGGAC CGCAACGAGG CCCGCGACCA CATGGTGCTC CTGGAGTCCT 
     4861 TCAGCGCCTG CTGCCACACC CACGGCATGG ACGAGCTGTA CAGGTCCGGA CTCAGATAAG 
     4921 TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT 
     4981 ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT 
     5041 CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 
     5101 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 
     5161 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 
     5221 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 
     5281 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 
     5341 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 
     5401 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 
     5461 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 
     5521 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 
     5581 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 
     5641 CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA 
     5701 GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG 
     5761 AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG 
     5821 TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC 
     5881 GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC 
     5941 AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC 
     6001 TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT 
     6061 TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT 
     6121 TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT 
     6181 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC 
     6241 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG 
     6301 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA 
     6361 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6421 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6481 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6541 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     6601 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     6661 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     6721 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     6781 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     6841 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     6901 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 
     6961 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7021 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7081 TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC 
     7141 GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT 
     7201 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT 
     7261 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT 
     7321 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG 
     7381 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA 
     7441 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG 
     7501 CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT 
     7561 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT 
     7621 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA 
     7681 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC 
     7741 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 
     7801 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 
     7861 AAAAGTGCCA CCTGAC
//