LOCUS       RC212866                8745 bp    DNA     circular  SYN 
DEFINITION  SH3TC2 (Myc-DDK-tagged)-Human SH3 domain and tetratricopeptide repeats 2 (SH3TC2)
ACCESSION   NM_024577
VERSION     NM_024577.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="SH3TC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGGCTGC TTCTGCATCC CCAGGGAGCG GAGTCTGACC CGGGGCCCAG 
     1081 GTAAAGAAAC TCCTTCCAAG GATCCAACTG TATCGAGTGA GTGTATAGCC TCATCTGAAT 
     1141 ACAAGGAAAA ATGTTTTCTG CCACAGAACA TTAATCCAGA CCTGACACTC TCCTTCTGTG 
     1201 TAAAGAGCCG CTCCAGGAGG TGTGTAAATG GACCCCTACA GGAAGCTGCT CGGAGGCGGC 
     1261 TCTGGGCACT GGAGAATGAG GACCAGGAGG TGCGCATGCT GTTTAAGGAC CTCTCAGCAA 
     1321 GGTTGGTCAG TATCCAGTCT CAGAGGGCCC AGTTTCTCAT CACCTTCAAG ACCATGGAGG 
     1381 AAATCTGGAA GTTCTCCACC TACCTTAATT TAGGCTACGT ATCCATGTGT CTAGAACATC 
     1441 TCCTCTTTGA CCACAAGTAC TGGCTCAACT GCATATTGGT GGAGGATACA GAGATCCAAG 
     1501 TGTCTGTAGA TGATAAACAC CTGGAAACAA TATACCTGGG ACTCCTGATA CAGGAAGGCC 
     1561 ACTTCTTCTG CAGAGCCCTG TGCTCCGTGA CTCCACCAGC CGAGAAGGAA GGGGAATGCT 
     1621 TGACACTTTG CAAGAATGAG TTAATCTCAG TGAAGATGGC AGAAGCTGGC TCCGAGTTGG 
     1681 AAGGCGTGTC TTTGGTGACA GGTCAGCGGG GCCTGGTACT GGTGTCAGCC TTGGAGCCTC 
     1741 TGCCTCTCCC TTTCCACCAG TGGTTCCTAA AGAATTATCC AGGAAGCTGT GGCCTTTCCA 
     1801 GGAAGAGGGA TTGGACAGGC TCCTATCAGA TTGGCAGAGG ACGCTGTAAG GCCTTGACGG 
     1861 GTTATGAGCC AGGAGAAAAG GATGAACTGA ATTTCTACCA GGGAGAAAGC ATTGAGATCA 
     1921 TCGGCTTTGT CATACCTGGG CTTCAGTGGT TCATTGGAAA GTCGACAAGT TCAGGACAAG 
     1981 TGGGCTTTGT CCCCACCAGG AACATAGATC CTGATTCTTA TTCCCCAATG AGCAGGAACT 
     2041 CTGCCTTTCT CAGTGATGAG GAGAGATGCT CCCTGTTGGC CCTGGGAAGT GATAAGCAGA 
     2101 CTGAGTGTTC CAGCTTCCTC CACACTCTTG CTCGCACTGA CATCACATCT GTCTACCGGC 
     2161 TCAGTGGGTT TGAATCCATC CAGAATCCTC CAAATGATCT GAGTGCATCC CAGCCTGAAG 
     2221 GCTTCAAGGA GGTCAGGCCT GGCAGAGCCT GGGAGGAGCA TCAGGCCGTG GGGTCCAGAC 
     2281 AGTCCAGCAG CTCTGAGGAC TCCAGCCTGG AGGAGGAGCT CCTCTCGGCC ACCTCAGACA 
     2341 GCTATCGCCT GCCGGAGCCT GATGACCTTG ATGACCCGGA ACTGCTCATG GACCTAAGCA 
     2401 CTGGTCAGGA GGAGGAGGCT GAGAACTTCT CCCCCATATT GGCTTTTCTG GATCATGAGG 
     2461 GTTATGCTGA CCACTTTAAG AGTCTCTATG ACTTCTCCTT CTCTTTCCTC ACTTCTTCCT 
     2521 TTTATAGCTT CTCTGAGGAG GATGAGTTTG TGGCCTACCT GGAGGCATCA AGAAAGTGGG 
     2581 CCAAGAAGAG CCACATGACC TGGGCCCATG CCCGGCTCTG CTTCCTCCTG GGCCGGCTGA 
     2641 GCATCAGGAA GGTCAAACTC TCTCAGGCCA GGGTGTACTT CGAGGAGGCC ATCCACATTC 
     2701 TCAATGGAGC ATTTGAGGAC CTATCCTTGG TGGCCACTCT GTACATCAAT TTGGCTGCCA 
     2761 TCTACCTGAA ACAGAGGCTG AGACATAAAG GCTCCGCCCT GTTGGAAAAG GCAGGTGCCC 
     2821 TGCTGGCCTG CCTGCCTGAC CGTGAGTCTA GTGCCAAGCA TGAACTCGAC GTGGTGGCCT 
     2881 ACGTGCTGCG CCAGGGGATT GTGGTGGGCA GCAGCCCGCT GGAGGCCAGG GCCTGCTTTC 
     2941 TGGCCATCCG CTTGCTCCTG AGCCTAGGCC GGCACGAGGA GGTCCTGCCC TTTGCCGAGC 
     3001 GCCTGCAGCT CCTCTCTGGA CACCCTCCTG CCTCTGAGGC TGTGGCCAGT GTTTTGAGTT 
     3061 TTCTGTATGA CAAGAAATAT CTTCCACACC TTGCAGTGGC CTCTGTCCAG CAACATGGTA 
     3121 TCCAGAGTGC CCAAGGGATG TCTCTTCCTA TTTGGCAGGT CCACCTTGTC CTCCAGAACA 
     3181 CAACCAAGCT CCTTGGCTTT CCTTCCCCAG GCTGGGGTGA AGTTTCTGCC TTGGCCTGCC 
     3241 CAATGCTCAG ACAGGCCCTG GCTGCCTGTG AGGAACTAGC AGACCGGAGC ACCCAGAGGG 
     3301 CCCTGTGTCT CATCCTTTCC AAAGTGTACC TCGAGCACAG GTCTCCTGAC GGTGCCATCC 
     3361 ACTACCTGAG CCAGGCCTTG GTGCTAGGGC AGCTGCTGGG TGAGCAGGAA TCCTTTGAGT 
     3421 CTTCTCTCTG CCTGGCATGG GCCTATCTCT TAGCCAGCCA GGCCAAGAAG GCTTTGGATG 
     3481 TGCTTGAGCC ACTGCTATGC TCCCTGAAGG AGACAGAGAG TCTCACTCAA AGGGGAGTCA 
     3541 TCTATAACCT CCTGGGACTT GCACTCCAAG GTGAAGGCCG GGTGAACAGG GCAGCCAAGA 
     3601 GCTATCTTCG GGCCTTGAAC AGAGCCCAGG AGGTGGGAGA TGTGCATAAC CAGGCAGTGG 
     3661 CTATGGCCAA TCTTGGCCAC CTGAGCCTTA AGTCCTGGGC TCAGCATCCA GCCAGAAACT 
     3721 ATCTCCTGCA GGCTGTACGA CTCTATTGTG AACTTCAGGC CAGTAAGGAG ACAGACATGG 
     3781 AATTAGTACA GGTGTTTCTC TGGTTGGCCC AAGTTCTGGT GTCTGGACAC CAGCTGACCC 
     3841 ATGGCCTTCT TTGTTATGAA ATGGCATTGC TGTTTGGCTT AAGGCATCGA CATCTAAAGA 
     3901 GTCAGCTTCA GGCCACCAAA TCCCTCTGCC ATTTCTACAG CTCTGTGTCC CCAAACCCTG 
     3961 AGGCATGCAT CACCTACCAT GAGCACTGGC TGGCCCTGGC TCAGCAACTC AGGGACCGGG 
     4021 AGATGGAAGG GAGGCTGCTG GAGTCCCTGG GGCAGCTTTA TCGGAACCTA AATACCGCCA 
     4081 GGTCCCTCAG GAGGTCACTC ACATGCATCA AGGAGAGCCT GCGTATCTTC ATTGACCTGG 
     4141 GGGAGACAGA CAAGGCTGCT GAGGCCTGGC TTGGGGCGGG GCGACTCCAC TACCTCATGC 
     4201 AGGAAGACGA GCTGGTGGAG CTGTGCCTGC AGGCAGCCAT CCAGACAGCC CTGAAGTCAG 
     4261 AGGAGCCTTT GCTGGCTCTC AAACTTTATG AAGAAGCAGG TGATGTGTTC TTCAATGGGA 
     4321 CCCGCCACAG GCATCATGCA GTGGAGTACT ACCGAGCTGG AGCTGTTCCT TTAGCAAGGA 
     4381 GGTTGAAGGC GGTGAGAACT GAGCTCCGGA TTTTCAATAA GCTGACAGAG CTGCAGATTA 
     4441 GCCTCGAAGG CTATGAGAAG GCTTTGGAAT TTGCCACCCT GGCCGCCAGG CTCAGCACAG 
     4501 TCACAGGAGA TCAGAGGCAA GAGCTGGTGG CCTTTCACCG CCTGGCTACA GTGTACTACT 
     4561 CCCTGCACAT GTATGAGATG GCTGAGGACT GCTACCTGAA GACCCTGTCC CTCTGTCCAC 
     4621 CCTGGCTGCA GAGTCCCAAG GAGGCCCTGT ACTATGCCAA GGTGTATTAT CGCCTGGGCA 
     4681 GACTCACCTT CTGCCAGCTG AAGGATGCCC ATGATGCCAC TGAGTACTTC CTTCTGGCCC 
     4741 TGGCAGCAGC GGTCCTGCTG GGTGATGAGG AGCTTCAGGA CACCATTAGG AGCAGGCTGG 
     4801 ACAACATCTG CCAGAGCCCC CTGTGGCACA GCAGGCCCTC CGGGTGCTCC TCAGAGAGGG 
     4861 CGCGGTGGCT GAGTGGTGGT GGCCTGGCCC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//