LOCUS       RC212825L1                9096 bp    DNA     circular  SYN 
DEFINITION  LGR5 (Myc-DDK-tagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)
ACCESSION   NM_003667
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7387..7567 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8295..8954 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7726..8294 
                     /label="ColE1"  
     misc_feature    6262..6282 
                     /label="LR50 primer"  
     misc_feature    6059..6130 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6271..6862 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6041..6047 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6041 
                     /label="LGR5"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACACCTCC CGGCTCGGTG TGCTCCTGTC 
     3361 CTTGCCTGTG CTGCTGCAGC TGGCGACCGG GGGCAGCTCT CCCAGGTCTG GTGTGTTGCT 
     3421 GAGGGGCTGC CCCACACACT GTCATTGCGA GCCCGACGGC AGGATGTTGC TCAGGGTGGA 
     3481 CTGCTCCGAC CTGGGGCTCT CGGAGCTGCC TTCCAACCTC AGCGTCTTCA CCTCCTACCT 
     3541 AGACCTCAGT ATGAACAACA TCAGTCAGCT GCTCCCGAAT CCCCTGCCCA GTCTCCGCTT 
     3601 CCTGGAGGAG TTACGTCTTG CGGGAAACGC TCTGACATAC ATTCCCAAGG GAGCATTCAC 
     3661 TGGCCTTTAC AGTCTTAAAG TTCTTATGCT GCAGAATAAT CAGCTAAGAC ACGTACCCAC 
     3721 AGAAGCTCTG CAGAATTTGC GAAGCCTTCA ATCCCTGCGT CTGGATGCTA ACCACATCAG 
     3781 CTATGTGCCC CCAAGCTGTT TCAGTGGCCT GCATTCCCTG AGGCACCTGT GGCTGGATGA 
     3841 CAATGCGTTA ACAGAAATCC CCGTCCAGGC TTTTAGAAGT TTATCGGCAT TGCAAGCCAT 
     3901 GACCTTGGCC CTGAACAAAA TACACCACAT ACCAGACTAT GCCTTTGGAA ACCTCTCCAG 
     3961 CTTGGTAGTT CTACATCTCC ATAACAATAG AATCCACTCC CTGGGAAAGA AATGCTTTGA 
     4021 TGGGCTCCAC AGCCTAGAGA CTTTAGATTT AAATTACAAT AACCTTGATG AATTCCCCAC 
     4081 TGCAATTAGG ACACTCTCCA ACCTTAAAGA ACTAGGATTT CATAGCAACA ATATCAGGTC 
     4141 GATACCTGAG AAAGCATTTG TAGGCAACCC TTCTCTTATT ACAATACATT TCTATGACAA 
     4201 TCCCATCCAA TTTGTTGGGA GATCTGCTTT TCAACATTTA CCTGAACTAA GAACACTGAC 
     4261 TCTGAATGGT GCCTCACAAA TAACTGAATT TCCTGATTTA ACTGGAACTG CAAACCTGGA 
     4321 GAGTCTGACT TTAACTGGAG CACAGATCTC ATCTCTTCCT CAAACCGTCT GCAATCAGTT 
     4381 ACCTAATCTC CAAGTGCTAG ATCTGTCTTA CAACCTATTA GAAGATTTAC CCAGTTTTTC 
     4441 AGTCTGCCAA AAGCTTCAGA AAATTGACCT AAGACATAAT GAAATCTACG AAATTAAAGT 
     4501 TGACACTTTC CAGCAGTTGC TTAGCCTCCG ATCGCTGAAT TTGGCTTGGA ACAAAATTGC 
     4561 TATTATTCAC CCCAATGCAT TTTCCACTTT GCCATCCCTA ATAAAGCTGG ACCTATCGTC 
     4621 CAACCTCCTG TCGTCTTTTC CTATAACTGG GTTACATGGT TTAACTCACT TAAAATTAAC 
     4681 AGGAAATCAT GCCTTACAGA GCTTGATATC ATCTGAAAAC TTTCCAGAAC TCAAGGTTAT 
     4741 AGAAATGCCT TATGCTTACC AGTGCTGTGC ATTTGGAGTG TGTGAGAATG CCTATAAGAT 
     4801 TTCTAATCAA TGGAATAAAG GTGACAACAG CAGTATGGAC GACCTTCATA AGAAAGATGC 
     4861 TGGAATGTTT CAGGCTCAAG ATGAACGTGA CCTTGAAGAT TTCCTGCTTG ACTTTGAGGA 
     4921 AGACCTGAAA GCCCTTCATT CAGTGCAGTG TTCACCTTCC CCAGGCCCCT TCAAACCCTG 
     4981 TGAACACCTG CTTGATGGCT GGCTGATCAG AATTGGAGTG TGGACCATAG CAGTTCTGGC 
     5041 ACTTACTTGT AATGCTTTGG TGACTTCAAC AGTTTTCAGA TCCCCTCTGT ACATTTCCCC 
     5101 CATTAAACTG TTAATTGGGG TCATCGCAGC AGTGAACATG CTCACGGGAG TCTCCAGTGC 
     5161 CGTGCTGGCT GGTGTGGATG CGTTCACTTT TGGCAGCTTT GCACGACATG GTGCCTGGTG 
     5221 GGAGAATGGG GTTGGTTGCC ATGTCATTGG TTTTTTGTCC ATTTTTGCTT CAGAATCATC 
     5281 TGTTTTCCTG CTTACTCTGG CAGCCCTGGA GCGTGGGTTC TCTGCGAAAT ATTCTGCAAA 
     5341 ATTTGAAACG AAAGCTCCAT TTTCTAGCCT GAAAGTAATC ATTTTGCTCT GTGCCCTGCT 
     5401 GGCCTTGACC ATGGCCGCAG TTCCCCTGCT GGGTGGCAGC AAGTATGGCG CCTCCCCTCT 
     5461 CTGCCTGCCT TTGCCTTTTG GGGAGCCCAG CACCATGGGC TACATGGTCG CTCTCATCTT 
     5521 GCTCAATTCC CTTTGCTTCC TCATGATGAC CATTGCCTAC ACCAAGCTCT ACTGCAATTT 
     5581 GGACAAGGGA GACCTGGAGA ATATTTGGGA CTGCTCTATG GTAAAACACA TTGCCCTGTT 
     5641 GCTCTTCACC AACTGCATCC TAAACTGCCC TGTGGCTTTC TTGTCCTTCT CCTCTTTAAT 
     5701 AAACCTTACA TTTATCAGTC CTGAAGTAAT TAAGTTTATC CTTCTGGTGG TAGTCCCACT 
     5761 TCCTGCATGT CTCAATCCCC TTCTCTACAT CTTGTTCAAT CCTCACTTTA AGGAGGATCT 
     5821 GGTGAGCCTG AGAAAGCAAA CCTACGTCTG GACAAGATCA AAACACCCAA GCTTGATGTC 
     5881 AATTAACTCT GATGATGTCG AAAAACAGTC CTGTGACTCA ACTCAAGCCT TGGTAACCTT 
     5941 TACCAGCTCC AGCATCACTT ATGACCTGCC TCCCAGTTCC GTGCCATCAC CAGCTTATCC 
     6001 AGTGACTGAG AGCTGCCATC TTTCCTCTGT GGCATTTGTC CCATGTCTCA CGCGTACGCG 
     6061 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     6121 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     6181 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     6241 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     6301 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     6361 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     6421 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     6481 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     6541 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     6601 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     6661 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     6721 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     6781 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     6841 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     6901 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     6961 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     7021 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     7081 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     7141 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     7201 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     7261 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     7321 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     7381 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     7441 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     7501 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     7561 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     7621 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     7681 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     7741 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     7801 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     7861 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     7921 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     7981 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     8041 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     8101 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     8161 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     8221 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     8281 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     8341 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     8401 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     8461 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     8521 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     8581 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     8641 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     8701 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     8761 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     8821 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     8881 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     8941 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     9001 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9061 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//