LOCUS       RC212782L1                8301 bp    DNA     circular  SYN 
DEFINITION  KLHL17 (Myc-DDK-tagged)-Human kelch-like 17 (Drosophila) (KLHL17)
ACCESSION   NM_198317
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6592..6772 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7500..8159 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6931..7499 
                     /label="ColE1"  
     misc_feature    5467..5487 
                     /label="LR50 primer"  
     misc_feature    5264..5335 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5476..6067 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5246..5252 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5246 
                     /label="KLHL17"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCAGCCCCGC AGCGAGCGCC CGGCCGGCAG 
     3361 GACGCAGAGC CCGGAGCACG GCAGCCCGGG GCCCGGGCCC GAGGCGCCGC CGCCTCCACC 
     3421 GCCGCAGCCG CCGGCCCCCG AGGCAGAGCG CACGCGGCCC CGGCAGGCTC GGCCCGCAGC 
     3481 CCCCATGGAG GGAGCCGTGC AGCTGCTGAG CCGCGAGGGC CACAGCGTGG CCCACAACTC 
     3541 CAAGCGGCAC TACCACGATG CCTTCGTGGC CATGAGCCGC ATGCGCCAGC GCGGCCTCCT 
     3601 GTGCGACATC GTCCTGCACG TGGCTGCCAA GGAGATCCGT GCGCACAAAG TGGTGCTGGC 
     3661 CTCCTGCAGC CCCTACTTCC ACGCCATGTT CACAAATGAG ATGAGCGAGA GCCGCCAGAC 
     3721 CCACGTGACG CTGCACGACA TCGACCCTCA GGCCTTGGAC CAGCTGGTGC AGTTTGCCTA 
     3781 CACGGCTGAG ATTGTGGTGG GCGAGGGCAA TGTGCAGACT CTGCTCCCAG CCGCCAGTCT 
     3841 CCTGCAGCTG AATGGCGTCC GAGACGCTTG CTGCAAGTTT CTACTGAGTC AGCTCGACCC 
     3901 CTCCAACTGC CTGGGTATCC GGGGCTTTGC CGATGCGCAC TCCTGCAGCG ACCTGCTCAA 
     3961 GGCCGCCCAC AGGTACGTGC TGCAGCACTT CGTGGACGTG GCCAAGACCG AGGAGTTTAT 
     4021 GCTGCTGCCC CTGAAACAGG TTCTGGAACT GGTCTCTAGC GACAGCCTGA ACGTGCCTTC 
     4081 AGAGGAGGAG GTCTACCGAG CCGTCCTGAG CTGGGTGAAA CACGACGTGG ACGCCCGCAG 
     4141 GCAGCATGTC CCACGGCTCA TGAAGTGTGT GCGGCTGCCC TTGCTGAGCC GCGACTTCCT 
     4201 GCTGGGCCAC GTGGATGCCG AGAGCCTGGT GAGGCACCAC CCTGACTGCA AGGACCTCCT 
     4261 CATCGAGGCC CTGAAGTTCC ACCTGCTGCC TGAGCAGAGG GGCGTCCTAG GCACCAGCCG 
     4321 CACACGTCCC CGGCGCTGCG AGGGGGCCGG GCCTGTGCTT TTTGCTGTGG GCGGCGGGAG 
     4381 CCTGTTTGCC ATCCACGGAG ACTGTGAGGC CTACGACACG CGCACCGACC GCTGGCACGT 
     4441 GGTGGCCTCC ATGTCCACGC GCCGGGCCCG GGTGGGAGTG GCTGCGGTGG GGAACCGGCT 
     4501 CTATGCTGTG GGCGGCTATG ATGGGACCTC AGACCTGGCT ACCGTGGAGT CCTACGACCC 
     4561 CGTGACTAAC ACGTGGCAGC CGGAGGTGTC CATGGGCACA AGGCGAAGCT GCCTGGGTGT 
     4621 GGCCGCCTTG CATGGACTCC TGTACTCGGC CGGCGGCTAT GACGGGGCCT CCTGCCTGAA 
     4681 CAGTGCTGAA CGCTACGACC CCCTGACCGG AACGTGGACG TCCGTCGCTG CCATGAGCAC 
     4741 CCGGAGGCGC TATGTGCGAG TGGCCACGCT TGATGGGAAC CTGTATGCTG TGGGCGGCTA 
     4801 CGACAGCTCC TCACACCTGG CCACTGTGGA GAAGTATGAG CCCCAGGTGA ACGTGTGGTC 
     4861 GCCCGTGGCG TCCATGCTGA GCCGACGCAG CTCAGCGGGC GTGGCCGTGC TGGAGGGTGC 
     4921 CCTGTACGTG GCAGGGGGCA ACGACGGCAC CAGCTGCCTC AACTCGGTAG AGAGATACAG 
     4981 TCCAAAGGCT GGAGCCTGGG AAAGCGTGGC GCCCATGAAT ATCCGCAGGA GCACGCATGA 
     5041 CCTGGTGGCC ATGGACGGAT GGTTGTACGC CGTGGGGGGT AACGACGGTA GCTCCAGCCT 
     5101 CAACTCCATC GAGAAGTACA ACCCGAGGAC CAACAAGTGG GTGGCCGCAT CCTGCATGTT 
     5161 CACCCGGCGC AGCAGTGTGG GTGTGGCGGT GCTGGAGCTG CTCAATTTCC CGCCGCCATC 
     5221 CTCCCCGACG CTGTCCGTGT CCTCCACCAG CCTCACGCGT ACGCGGCCGC TCGAGCAGAA 
     5281 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     5341 TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     5401 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     5461 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     5521 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     5581 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     5641 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     5701 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     5761 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     5821 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     5881 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     5941 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     6001 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     6061 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     6121 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     6181 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     6241 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     6301 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     6361 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     6421 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     6481 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     6541 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     6601 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     6661 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     6721 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     6781 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6841 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6901 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6961 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     7021 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     7081 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     7141 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     7201 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     7261 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     7321 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7381 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7441 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7501 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     7561 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     7621 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     7681 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     7741 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     7801 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     7861 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     7921 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     7981 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     8041 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     8101 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     8161 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8221 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTCCC GCGCACATTT 
     8281 CCCCGAAAAG TGCCACCTGA C
//