LOCUS       RC212777                6681 bp    DNA     circular  SYN 
DEFINITION  IFT74 (Myc-DDK-tagged)-Human intraflagellar transport 74 homolog (Chlamydomonas) (IFT74), transcript variant 2
ACCESSION   NM_001099222
VERSION     NM_001099222.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2838..2929 
                     /label="Myc-DDK"  
     misc_feature    2959..3544 
                     /label="PolyA signal"  
     misc_feature    3016..3035 
                     /label="Restriction Enzyme"  
     misc_feature    3820..4492 
                     /label="ColE1"  
     misc_feature    4922..5716 
                     /label="Kan/Neo"  
     misc_feature    5751..6053 
                     /label="SV40 ori"  
     misc_feature    6139..6616 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2820..2826 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2820 
                     /label="IFT74"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGCAAT CACAAATCTT CAGCAGCTCG CCCTGTTTCA AGAGGTGGAG 
     1081 TTGGGTTAAC AGGAAGGCCT CCTTCTGGGA TACGACCCCT ATCAGGAAAT ATTCGAGTGG 
     1141 CAACTGCAAT GCCACCTGGG ACAGCAAGAC CAGGTTCTCG TGGTTGTCCC ATAGGGACTG 
     1201 GTGGAGTTCT GTCTTCTCAA ATCAAAGTTG CCCATCGCCC TGTAACACAA CAAGGTTTGA 
     1261 CTGGAATGAA AACTGGGACG AAAGGTCCCC AGAGGCAAAT TTTAGACAAA TCTTACTATC 
     1321 TTGGGCTTCT TAGAAGTAAA ATAAGTGAAC TTACAACTGA AGTTAATAAA CTTCAGAAGG 
     1381 GAATAGAAAT GTACAATCAA GAGAATTCAG TATATTTGTC ATATGAAAAG AGGGCTGAGA 
     1441 CTTTAGCTGT TGAGATAAAA GAGCTTCAAG GACAACTAGC AGACTACAAC ATGTTGGTAG 
     1501 ATAAACTTAA TACCAACACT GAAATGGAAG AAGTAATGAA TGATTACAAT ATGCTTAAAG 
     1561 CTCAAAATGA TCGAGAAACA CAAAGTTTGG ATGTCATATT TACTGAAAGA CAAGCGAAAG 
     1621 AAAAACAAAT CAGAAGTGTC GAAGAAGAAA TTGAACAGGA AAAACAAGCA ACAGATGACA 
     1681 TTATCAAAAA TATGTCTTTT GAAAACCAAG TCAAGTACCT AGAGATGAAA ACCACAAATG 
     1741 AGAAACTGTT ACAGGAATTA GATACACTTC AACAACAATT GGATTCACAG AACATGAAAA 
     1801 AAGAGAGCCT GGAAGCAGAA ATAGCTCACT CCCAGGTGAA ACAGGAGGCG GTATTGCTGC 
     1861 ATGAAAAACT TTATGAGTTG GAGTCCCATC GAGATCAAAT GATTGCAGAA GACAAAAGCA 
     1921 TAGGATCTCC AATGGAAGAG AGAGAGAAAT TACTTAAGCA GATTAAAGAT GATAATCAGG 
     1981 AAATAGCCAG CATGGAAAGA CAGTTAACAG ATACAAAAGA AAAGATAAAT CAGTTTATTG 
     2041 AAGAAATTAG ACAACTTGAC ATGGATTTAG AGGAACACCA AGGTGAAATG AACCAGAAAT 
     2101 ACAAGGAGCT AAAGAAAAGG GAGGAACATA TGGACACTTT TATTGAGACT TTTGAGGAAA 
     2161 CAAAGAATCA GGAACTGAAA CGAAAGGCAC AGATAGAAGC CAACATTGTT GCACTCTTGG 
     2221 AGCACTGCAG TCGAAATATA AATCGTATAG AACAGATATC CTCTATCACC AATCAAGAGC 
     2281 TAAAGATGAT GCAGGATGAC CTCAATTTTA AATCTACTGA AGTGCAGAAA TCACAAAGTA 
     2341 CAGCTCAGAA TTTGACTTCA GACATTCAAC GTCTGCAGTT GGATCTGCAG AAAATGGAGC 
     2401 TTCTAGAAAG TAAGATGACT GAAGAACAGC ATTCTCTAAA AAGCAAAATT AAGCAAATGA 
     2461 CAACTGATCT GGAGATATAT AATGATTTGC CAGCTTTAAA ATCATCAGGT GAAGAAAAGA 
     2521 TAAAGAAATT ACATCAGGAG AGAATGATAT TATCAACCCA CAGAAATGCC TTTAAGAAAA 
     2581 TAATGGAGAA GCAAAACATA GAGTATGAGG CACTAAAAAC ACAATTGCAA GAAAATGAGA 
     2641 CACATTCTCA GCTTACAAAT TTGGAGAGAA AGTGGCAACA CCTTGAGCAA AATAATTTTG 
     2701 CGATGAAAGA ATTCATAGCA ACCAAGAGTC AAGAGAGTGA TTACCAGCCA ATTAAGAAAA 
     2761 ATGTGACCAA GCAGATTGCA GAGTACAATA AAACCATCGT GGATGCTTTA CATAGCACCA 
     2821 GCGGAAACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     2881 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     2941 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3001 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3061 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3121 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3181 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3241 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3301 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3361 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3421 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3481 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3541 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3601 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3661 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3721 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     3781 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     3841 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     3901 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     3961 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4021 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4081 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4141 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4201 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4261 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4321 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4381 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4441 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4501 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4561 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4621 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4681 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     4741 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     4801 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     4861 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     4921 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     4981 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5041 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5101 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5161 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5221 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5281 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5341 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5401 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5461 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5521 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5581 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5641 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     5701 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     5761 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     5821 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     5881 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     5941 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6001 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6061 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6121 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6181 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6241 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6301 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6361 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6421 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6481 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6541 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6601 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6661 CAAAAATTTA ACGCGAATTT T
//