LOCUS       RC212675                7491 bp    DNA     circular  SYN 
DEFINITION  SULF2 (Myc-DDK-tagged)-Human sulfatase 2 (SULF2), transcript variant 1
ACCESSION   NM_018837
VERSION     NM_018837.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3648..3739 
                     /label="Myc-DDK"  
     misc_feature    3769..4354 
                     /label="PolyA signal"  
     misc_feature    3826..3845 
                     /label="Restriction Enzyme"  
     misc_feature    4630..5302 
                     /label="ColE1"  
     misc_feature    5732..6526 
                     /label="Kan/Neo"  
     misc_feature    6561..6863 
                     /label="SV40 ori"  
     misc_feature    6949..7426 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3630..3636 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3630 
                     /label="SULF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCCCCCG AGCCTCGTGC TGTGCTTGCT GTCCGCAACT GTGTTCTCCC 
     1081 TGCTGGGTGG AAGCTCGGCC TTCCTGTCGC ACCACCGCCT GAAAGGCAGG TTTCAGAGGG 
     1141 ACCGCAGGAA CATCCGCCCC AACATCATCC TGGTGCTGAC GGACGACCAG GATGTGGAGC 
     1201 TGGGTTCCAT GCAGGTGATG AACAAGACCC GGCGCATCAT GGAGCAGGGC GGGGCGCACT 
     1261 TCATCAACGC CTTCGTGACC ACACCCATGT GCTGCCCCTC ACGCTCCTCC ATCCTCACTG 
     1321 GCAAGTACGT CCACAACCAC AACACCTACA CCAACAATGA GAACTGCTCC TCGCCCTCCT 
     1381 GGCAGGCACA GCACGAGAGC CGCACCTTTG CCGTGTACCT CAATAGCACT GGCTACCGGA 
     1441 CAGCTTTCTT CGGGAAGTAT CTTAATGAAT ACAACGGCTC CTACGTGCCA CCCGGCTGGA 
     1501 AGGAGTGGGT CGGACTCCTT AAAAACTCCC GCTTTTATAA CTACACGCTG TGTCGGAACG 
     1561 GGGTGAAAGA GAAGCACGGC TCCGACTACT CCAAGGATTA CCTCACAGAC CTCATCACCA 
     1621 ATGACAGCGT GAGCTTCTTC CGCACGTCCA AGAAGATGTA CCCGCACAGG CCAGTCCTCA 
     1681 TGGTCATCAG CCATGCAGCC CCCCACGGCC CTGAGGATTC AGCCCCACAA TATTCACGCC 
     1741 TCTTCCCAAA CGCATCTCAG CACATCACGC CGAGCTACAA CTACGCGCCC AACCCGGACA 
     1801 AACACTGGAT CATGCGCTAC ACGGGGCCCA TGAAGCCCAT CCACATGGAA TTCACCAACA 
     1861 TGCTCCAGCG GAAGCGCTTG CAGACCCTCA TGTCGGTGGA CGACTCCATG GAGACGATTT 
     1921 ACAACATGCT GGTTGAGACG GGCGAGCTGG ACAACACGTA CATCGTATAC ACCGCCGACC 
     1981 ACGGTTACCA CATCGGCCAG TTTGGCCTGG TGAAAGGGAA ATCCATGCCA TATGAGTTTG 
     2041 ACATCAGGGT CCCGTTCTAC GTGAGGGGCC CCAACGTGGA AGCCGGCTGT CTGAATCCCC 
     2101 ACATCGTCCT CAACATTGAC CTGGCCCCCA CCATCCTGGA CATTGCAGGC CTGGACATAC 
     2161 CTGCGGATAT GGACGGGAAA TCCATCCTCA AGCTGCTGGA CACGGAGCGG CCGGTGAATC 
     2221 GGTTTCACTT GAAAAAGAAG ATGAGGGTCT GGCGGGACTC CTTCTTGGTG GAGAGAGGCA 
     2281 AGCTGCTACA CAAGAGAGAC AATGACAAGG TGGACGCCCA GGAGGAGAAC TTTCTGCCCA 
     2341 AGTACCAGCG TGTGAAGGAC CTGTGTCAGC GTGCTGAGTA CCAGACGGCG TGTGAGCAGC 
     2401 TGGGACAGAA GTGGCAGTGT GTGGAGGACG CCACGGGGAA GCTGAAGCTG CATAAGTGCA 
     2461 AGGGCCCCAT GCGGCTGGGC GGCAGCAGAG CCCTCTCCAA CCTCGTGCCC AAGTACTACG 
     2521 GGCAGGGCAG CGAGGCCTGC ACCTGTGACA GCGGGGACTA CAAGCTCAGC CTGGCCGGAC 
     2581 GCCGGAAAAA ACTCTTCAAG AAGAAGTACA AGGCCAGCTA TGTCCGCAGT CGCTCCATCC 
     2641 GCTCAGTGGC CATCGAGGTG GACGGCAGGG TGTACCACGT AGGCCTGGGT GATGCCGCCC 
     2701 AGCCCCGAAA CCTCACCAAG CGGCACTGGC CAGGGGCCCC TGAGGACCAA GATGACAAGG 
     2761 ATGGTGGGGA CTTCAGTGGC ACTGGAGGCC TTCCCGACTA CTCAGCCGCC AACCCCATTA 
     2821 AAGTGACACA TCGGTGCTAC ATCCTAGAGA ACGACACAGT CCAGTGTGAC CTGGACCTGT 
     2881 ACAAGTCCCT GCAGGCCTGG AAAGACCACA AGCTGCACAT CGACCACGAG ATTGAAACCC 
     2941 TGCAGAACAA AATTAAGAAC CTGAGGGAAG TCCGAGGTCA CCTGAAGAAA AAGCGGCCAG 
     3001 AAGAATGTGA CTGTCACAAA ATCAGCTACC ACACCCAGCA CAAAGGCCGC CTCAAGCACA 
     3061 GAGGCTCCAG TCTGCATCCT TTCAGGAAGG GCCTGCAAGA GAAGGACAAG GTGTGGCTGT 
     3121 TGCGGGAGCA GAAGCGCAAG AAGAAACTCC GCAAGCTGCT CAAGCGCCTG CAGAACAACG 
     3181 ACACGTGCAG CATGCCAGGC CTCACGTGCT TCACCCACGA CAACCAGCAC TGGCAGACGG 
     3241 CGCCTTTCTG GACACTGGGG CCTTTCTGTG CCTGCACCAG CGCCAACAAT AACACGTACT 
     3301 GGTGCATGAG GACCATCAAT GAGACTCACA ATTTCCTCTT CTGTGAATTT GCAACTGGCT 
     3361 TCCTAGAGTA CTTTGATCTC AACACAGACC CCTACCAGCT GATGAATGCA GTGAACACAC 
     3421 TGGACAGGGA TGTCCTCAAC CAGCTACACG TACAGCTCAT GGAGCTGAGG AGCTGCAAGG 
     3481 GTTACAAGCA GTGTAACCCC CGGACTCGAA ACATGGACCT GGGACTTAAA GATGGAGGAA 
     3541 GCTATGAGCA ATACAGGCAG TTTCAGCGTC GAAAGTGGCC AGAAATGAAG AGACCTTCTT 
     3601 CCAAATCACT GGGACAACTG TGGGAAGGCT GGGAAGGTAC GCGTACGCGG CCGCTCGAGC 
     3661 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3721 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3781 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3841 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3901 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3961 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4021 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4081 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4141 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4201 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4261 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4321 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4381 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4441 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4501 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5161 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5221 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5281 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5341 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5401 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5461 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5521 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5581 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5641 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5701 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5761 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5821 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5881 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5941 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6001 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6061 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6121 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6181 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6241 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6301 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6361 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6421 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6481 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6541 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6601 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6661 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6721 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6901 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6961 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7021 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7081 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7141 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7201 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7261 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7321 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7381 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7441 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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