LOCUS       RC212621                6765 bp    DNA     circular  SYN 
DEFINITION  KLHL14 (Myc-DDK-tagged)-Human kelch-like 14 (Drosophila) (KLHL14)
ACCESSION   NM_020805
VERSION     NM_020805.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2922..3013 
                     /label="Myc-DDK"  
     misc_feature    3043..3628 
                     /label="PolyA signal"  
     misc_feature    3100..3119 
                     /label="Restriction Enzyme"  
     misc_feature    3904..4576 
                     /label="ColE1"  
     misc_feature    5006..5800 
                     /label="Kan/Neo"  
     misc_feature    5835..6137 
                     /label="SV40 ori"  
     misc_feature    6223..6700 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2904..2910 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2904 
                     /label="KLHL14"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGATCC GGGGACAGGA CCTCCACCTT CGACCCCAGC CACAGCGACA 
     1081 ACCTGCTGCA CGGCCTCAAC CTGCTGTGGA GGAAGCAGCT GTTTTGCGAC GTGACCCTGA 
     1141 CGGCCCAGGG CCAGCAGTTC CATTGCCACA AGGCCGTGCT GGCCTCCTGC TCGCAGTACT 
     1201 TCCGATCGCT CTTCTCCAGC CACCCCCCTC TCGGGGGAGG GGTCGGCGGC CAGGACGGCC 
     1261 TGGGGGCCCC CAAGGACCAG CAGCAGCCGC CGCAGCAGCA GCCGTCACAG CAGCAGCAGC 
     1321 CGCCGCCGCA GGAGGAGCCC GGGACTCCTT CTTCCTCCCC CGACGACAAG CTGCTGACCA 
     1381 GCCCCCGGGC CATCAACAAC CTGGTGCTGC AGGGCTGCTC GTCCATCGGG CTGCGCCTGG 
     1441 TGCTCGAGTA CCTCTACACG GCCAACGTGA CCCTGTCCCT GGACACGGTG GAGGAGGTGC 
     1501 TGTCGGTCAG CAAGATCCTG CACATCCCCC AGGTCACCAA GCTCTGCGTG CAGTTCCTCA 
     1561 ACGACCAGAT CTCGGTGCAG AACTACAAGC AGGTGTGCAA GATCGCCGCG CTGCACGGCC 
     1621 TGGAGGAGAC CAAGAAGCTG GCCAACAAGT ACCTGGTGGA GGATGTGCTG CTGCTCAACT 
     1681 TCGAGGAGAT GCGCGCCCTG CTGGACTCGC TGCCGCCCCC CGTGGAGTCG GAGCTGGCGC 
     1741 TCTTCCAGAT GTCCGTGCTG TGGCTGGAGC ACGACCGCGA GACCCGCATG CAGTATGCGC 
     1801 CTGACCTCAT GAAGCGCCTC CGCTTCGCCC TCATCCCGGC CCCGGAGCTG GTGGAGCGGG 
     1861 TCCAGTCAGT GGATTTCATG CGAACCGACC CGGTCTGCCA GAAGCTGCTG CTGGACGCCA 
     1921 TGAACTACCA CCTGATGCCC TTCAGGCAGC ACTGCAGGCA GAGCCTGGCC AGCAGAATTC 
     1981 GCTCTAACAA GAAAATGCTG TTATTGGTTG GAGGGCTGCC TCCTGGACCG GACCGGCTCC 
     2041 CCAGCAATTT GGTTCAGTAT TACGACGATG AAAAGAAGAC ATGGAAAATA CTCACAATTA 
     2101 TGCCATACAA CAGTGCCCAC CACTGCGTTG TGGAGGTGGA AAACTTCTTG TTCGTGTTGG 
     2161 GTGGAGAGGA CCAGTGGAAT CCGAATGGAA AACACAGTAC AAATTTTGTC AGCCGATATG 
     2221 ATCCTCGATT TAATAGCTGG ATTCAACTTC CACCCATGCA GGAAAGAAGA GCCAGTTTCT 
     2281 ATGCATGTCG GTTGGACAAG CATTTATACG TAATTGGTGG AAGGAATGAA ACTGGCTACT 
     2341 TGTCCAGCGT GGAGTGCTAT AACCTAGAAA CGAATGAATG GCGCTATGTG TCCTCTTTGC 
     2401 CACAGCCTCT GGCGGCTCAT GCGGGAGCAG TGCACAATGG GAAAATATAC ATTTCAGGGG 
     2461 GTGTACACAA TGGAGAATAT GTCCCATGGC TATATTGCTA TGACCCAGTA ATGGATGTCT 
     2521 GGGCTCGAAA ACAAGATATG AACACAAAAC GTGCAATTCA CACTTTGGCT GTAATGAATG 
     2581 ATCGCTTGTA TGCAATTGGA GGAAATCATT TGAAAGGTTT CTCCCACCTT GATGTAATGC 
     2641 TTGTGGAATG CTATGACCCA AAAGGTGACC AGTGGAATAT ACTCCAAACT CCCATTTTGG 
     2701 AGGGTCGAAG TGGCCCTGGC TGTGCAGTGC TTGATGACAG CATTTACCTT GTGGGAGGCT 
     2761 ACAGCTGGAG TATGGGGGCC TACAAGTCAT CTACAATATG CTATTGTCCA GAGAAAGGAA 
     2821 CCTGGACAGA ACTCGAAGGA GATGTAGCAG AACCGTTGGC AGGCCCTGCC TGTGTGACAG 
     2881 TTATTCTGCC CTCTTGTGTA CCATACAACA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     2941 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3001 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3061 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3121 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3181 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3241 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3301 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3361 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3421 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3481 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3541 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3601 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3661 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3721 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3781 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3841 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3901 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     3961 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4021 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4081 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4141 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4201 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4261 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4321 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4381 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4441 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4501 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4561 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4621 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4681 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4741 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4801 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4861 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4921 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     4981 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5041 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5101 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5161 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5221 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5281 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5341 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5401 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5461 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5521 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5581 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5641 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5701 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5761 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5821 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5881 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     5941 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6001 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6061 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6121 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6181 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6301 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6361 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6421 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6481 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6541 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6601 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6661 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6721 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//