LOCUS       RC212581                7803 bp    DNA     circular  SYN 
DEFINITION  PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 1
ACCESSION   NM_000919
VERSION     NM_000919.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3960..4051 
                     /label="Myc-DDK"  
     misc_feature    4081..4666 
                     /label="PolyA signal"  
     misc_feature    4138..4157 
                     /label="Restriction Enzyme"  
     misc_feature    4942..5614 
                     /label="ColE1"  
     misc_feature    6044..6838 
                     /label="Kan/Neo"  
     misc_feature    6873..7175 
                     /label="SV40 ori"  
     misc_feature    7261..7738 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3948 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="PAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCCGC GTCCCTAGCC TGCTAGTTCT CCTTGTTTTT CCAAGCAGCT 
     1081 GTTTGGCTTT CCGAAGCCCA CTTTCTGTCT TTAAGAGGTT TAAAGAAACT ACCAGACCAT 
     1141 TTTCCAATGA ATGTCTTGGT ACCACCAGAC CCGTAGTTCC TATTGATTCA TCAGATTTTG 
     1201 CATTGGATAT TCGCATGCCT GGGGTTACAC CTAAACAGTC CGATACATAC TTCTGCATGT 
     1261 CTATGCGAAT ACCAGTGGAT GAGGAAGCCT TCGTGATTGA CTTCAAGCCT CGAGCCAGCA 
     1321 TGGATACTGT CCATCACATG TTACTTTTTG GATGCAATAT GCCTTCATCC ACTGGAAGTT 
     1381 ACTGGTTTTG TGATGAAGGA ACCTGTACAG ATAAAGCCAA TATTCTGTAT GCCTGGGCGA 
     1441 GAAATGCTCC CCCTACCCGG CTCCCCAAAG GTGTTGGATT CAGAGTTGGA GGAGAGACTG 
     1501 GAAGTAAATA CTTTGTACTA CAGGTACACT ATGGGGATAT TAGTGCTTTT AGAGATAATA 
     1561 ACAAGGACTG TTCTGGTGTG TCCTTACACC TCACACGTCT GCCACAGCCT TTAATTGCTG 
     1621 GCATGTACCT TATGATGTCT GTTGACACTG TTATCCCAGC AGGAGAAAAA GTGGTGAATT 
     1681 CTGACATTTC ATGCCATTAT AAAAATTATC CAATGCATGT CTTTGCCTAT AGAGTTCACA 
     1741 CTCACCATTT AGGTAAGGTA GTAAGTGGAT ACAGAGTAAG AAATGGACAG TGGACACTGA 
     1801 TTGGACGGCA GAGCCCTCAG CTGCCACAGG CTTTCTACCC TGTGGGGCAT CCAGTTGATG 
     1861 TAAGTTTTGG TGACCTACTG GCTGCAAGAT GTGTATTCAC TGGTGAAGGA AGGACAGAAG 
     1921 CCACACACAT TGGTGGCACG TCTAGTGATG AAATGTGCAA CTTATACATT ATGTATTACA 
     1981 TGGAAGCCAA GCATGCAGTT TCTTTCATGA CCTGTACCCA GAATGTAGCT CCAGATATGT 
     2041 TCAGAACCAT ACCACCAGAG GCCAACATTC CAATTCCCGT GAAGTCTGAT ATGGTTATGA 
     2101 TGCATGAACA TCATAAAGAA ACAGAATATA AAGATAAGAT TCCTTTACTA CAGCAGCCAA 
     2161 AACGAGAAGA AGAAGAAGTG TTAGACCAGG GTGATTTCTA TTCACTACTT TCCAAGCTGC 
     2221 TAGGAGAAAG GGAAGATGTT GTTCATGTGC ACAAATATAA TCCTACAGAA AAGGCAGAAT 
     2281 CAGAGTCAGA CCTGGTAGCT GAGATTGCAA ATGTAGTCCA AAAAAAGGAT CTTGGTCGAT 
     2341 CTGATGCCAG AGAGGGTGCA GAACATGAGA GGGGTAATGC TATTCTTGTC AGAGACAGAA 
     2401 TTCACAAATT CCACAGACTA GTATCTACCT TGAGGCCACC AGAGAGCAGA GTTTTCTCAT 
     2461 TACAGCAGCC CCCACCTGGT GAAGGCACCT GGGAACCAGA ACACACAGGA GATTTCCACA 
     2521 TGGAAGAGGC ACTGGATTGG CCTGGAGTAT ACTTGTTACC AGGCCAGGTT TCTGGGGTGG 
     2581 CTCTAGACCC TAAGAATAAC CTGGTGATTT TCCACAGAGG TGACCATGTC TGGGATGGAA 
     2641 ACTCGTTTGA CAGCAAGTTT GTTTACCAGC AAATAGGACT CGGACCAATT GAAGAAGACA 
     2701 CTATTCTTGT CATAGATCCA AATAATGCTG CAGTACTCCA GTCCAGTGGA AAAAATCTGT 
     2761 TTTACTTGCC ACATGGCTTG AGTATAGATA AAGATGGGAA TTATTGGGTC ACAGACGTGG 
     2821 CTCTCCATCA GGTGTTCAAA CTGGATCCAA ACAATAAAGA AGGCCCTGTA TTAATCCTGG 
     2881 GAAGGAGCAT GCAACCAGGC AGTGACCAGA ATCACTTCTG TCAACCCACT GATGTGGCTG 
     2941 TGGATCCAGG CACTGGAGCC ATTTATGTAT CAGATGGTTA CTGCAACAGC AGGATTGTGC 
     3001 AGTTTTCACC AAGTGGAAAG TTCATCACAC AGTGGGGAGA AGAGTCTTCA GGGAGCAGTC 
     3061 CTCTGCCAGG CCAGTTCACT GTTCCTCACA GCTTGGCTCT TGTGCCTCTT TTGGGCCAAT 
     3121 TATGTGTGGC AGACCGGGAA AATGGTCGGA TCCAGTGTTT TAAAACTGAC ACCAAAGAAT 
     3181 TTGTGAGAGA GATTAAGCAT TCATCATTTG GAAGAAATGT ATTTGCAATT TCATATATAC 
     3241 CAGGCTTGCT CTTTGCAGTG AATGGGAAGC CTCATTTTGG GGACCAAGAA CCTGTACAAG 
     3301 GATTTGTGAT GAACTTTTCC AATGGGGAAA TTATAGACAT CTTCAAGCCA GTGCGCAAGC 
     3361 ACTTTGATAT GCCTCATGAT ATTGTTGCAT CTGAAGATGG GACTGTGTAC ATTGGAGATG 
     3421 CTCATACCAA CACCGTGTGG AAGTTCACCT TGACTGAGAA ATTGGAACAT CGATCAGTTA 
     3481 AAAAGGCTGG CATTGAGGTC CAGGAAATCA AAGAAGCCGA GGCAGTTGTT GAAACCAAAA 
     3541 TGGAGAACAA ACCCACCTCC TCAGAATTGC AGAAGATGCA AGAGAAACAG AAACTGATCA 
     3601 AAGAGCCAGG CTCGGGAGTG CCTGTTGTTC TCATTACAAC CCTTCTGGTT ATTCCGGTGG 
     3661 TTGTCCTGCT GGCCATTGCC ATATTTATTC GGTGGAAAAA ATCAAGGGCC TTTGGAGCAG 
     3721 ATTCTGAACA CAAACTCGAG ACGAGTTCAG GAAGAGTACT GGGAAGATTT AGAGGAAAGG 
     3781 GAAGTGGAGG CTTAAACCTT GGTAATTTCT TTGCAAGCCG TAAGGGCTAC AGTCGAAAAG 
     3841 GGTTTGACCG GCTTAGCACT GAGGGCAGTG ACCAAGAGAA AGAGGATGAT GGAAGTGAAT 
     3901 CAGAAGAGGA GTATTCAGCA CCTCTGCCTG CGCTCGCACC TTCCTCCTCC ACGCGTACGC 
     3961 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4021 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4081 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4141 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4201 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4261 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4321 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4381 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4441 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4501 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4561 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4621 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4681 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4741 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4801 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4861 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4921 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4981 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5041 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5101 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5161 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5221 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5281 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5341 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5401 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5461 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5521 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5581 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5641 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5701 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5761 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5821 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5881 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5941 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6001 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6061 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6121 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6181 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6241 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6301 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6361 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6421 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6481 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6541 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6601 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6661 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6721 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6781 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6841 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6901 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6961 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7021 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7081 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7141 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7201 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7261 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7321 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7381 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7441 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7501 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7561 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7621 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7681 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7741 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7801 TTT
//