LOCUS       RC212567                6636 bp    DNA     circular  SYN 
DEFINITION  PGBD4 (Myc-DDK-tagged)-Human piggyBac transposable element derived 4 (PGBD4)
ACCESSION   NM_152595
VERSION     NM_152595.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2793..2884 
                     /label="Myc-DDK"  
     misc_feature    2914..3499 
                     /label="PolyA signal"  
     misc_feature    2971..2990 
                     /label="Restriction Enzyme"  
     misc_feature    3775..4447 
                     /label="ColE1"  
     misc_feature    4877..5671 
                     /label="Kan/Neo"  
     misc_feature    5706..6008 
                     /label="SV40 ori"  
     misc_feature    6094..6571 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2775..2781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2775 
                     /label="PGBD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAAATCCT AGAAAACGTA GCATTCCTAT GCGTGATAGT AATACCGGTC 
     1081 TCGAACAGTT GTTGGCTGAA GATTCATTTG ATGAATCTGA TTTTTCGGAA ATAGATGATT 
     1141 CTGATAATTT TTCGGATAGT GCTTTAGAAG CCGATAAGAT CAGGCCTCTG TCCCATTTAG 
     1201 AATCTGATGG AAAGAGCTCT ACATCAAGTG ACTCAGGGCG CTCCATGAAA TGGTCAGCTC 
     1261 GTGCTATGAT TCCACGTCAA AGGTATGACT TTACCGGCAC ACCTGGCAGA AAAGTCGATG 
     1321 TCAGTGATAT CACTGACCCA TTGCAGTATT TTGAACTGTT CTTTACTGAG GAATTAGTTT 
     1381 CAAAAATTAC TAGAGAAACA AATGCCCAAG CTGCCTTGTT GGCTTCAAAG CCACCGGGTC 
     1441 CGAAAGGATT TTCGCGAATG GATAAATGGA AAGACACTGA CAATGACGAG CTCAAAGTCT 
     1501 TTTTTGCAGT AATGTTACTG CAAGGTATTG TGCAGAAACC TGAGCTGGAG ATGTTTTGGT 
     1561 CAACAAGGCC TCTTTTGGAT ACACCTTATC TCAGGCAAAT TATGACTGGT GAAAGATTTT 
     1621 TACTTTTGTT TCGGTGCCTG CATTTTGTCA ACAATTCTTC TATATCTGCT GGTCAATCAA 
     1681 AGGCCCAGAT TTCATTGCAG AAGATCAAAC CTGTGTTCGA CTTTCTTGTA AATAAATTTT 
     1741 CCACTGTATA TACTCCAAAC AGAAACATTG CAGTTGATGA ATCACTGATG CTGTTCAAGG 
     1801 GGCCATTAGC TATGAAGCAG TACCTCCCGA CAAAACGAGT ACGATTTGGT CTGAAGCTAT 
     1861 ATGTACTTTG TGAAAGTCAG TCTGGTTATG TGTGGAATGC GCTTGTTCAC ACAGGGCCTG 
     1921 GCATGAATTT GAAAGATTCA GCGGATGGCC TGAAATCATC ACGCATTGTT CTTACCTTGG 
     1981 TCAATGACCT TCTTGGCCAA GGGTATTGTG TCTTCCTCGA TAACTTTAAT ATATCTCCCA 
     2041 TGCTTTTCAG AGAATTACAT CAAAATAGGA CTGATGCAGT TGGGACAGCT CGTTTGAACA 
     2101 GAAAACAGAT TCCAAATGAT CTGAAAAAAA GGATTGCAAA GGGGACGACT GTAGCCAGAT 
     2161 TCTGTGGTGA ACTTATGGCA CTGAAATGGT GTGACGGCAA GGAGGTGACA ATGTTGTCAA 
     2221 CATTCCACAA TGATACTGTG ATTGAAGTAA ACAATAGAAA TGGAAAGAAA ACTAAAAGGC 
     2281 CACGTGTCAT TGTGGATTAT AACGAGAATA TGGGAGCAGT GGACTCGGCT GATCAAATGC 
     2341 TTACTTCTTA TCCATCTGAG CGCAAAAGAC ACAAGGTTTG GTATAAGAAA TTCTTTCACC 
     2401 ATCTTCTACA CATTACAGTG CTGAACTCCT ACATCCTGTT CAAGAAGGAT AATCCTGAGC 
     2461 ACACGATGAG CCATATAAAC TTCAGACTGG CATTGATTGA AAGAATGCTG GAAAAGCATC 
     2521 ACAAGCCAGG GCAGCAACAT CTTCGAGGTC GTCCTTGCTC CGATGATGTC ACACCTCTTC 
     2581 GTCTGTCTGG AAGACATTTC CCCAAGAGCA TACCAGCAAC GTCCGGGAAA CAGAATCCAA 
     2641 CTGGTCGCTG CAAAATTTGC TGCTCCCAAT ACGACAAGGA TGGCAAGAAG ATCCGGAAAG 
     2701 AAACGCGCTA TTTTTGTGCC GAATGTGATG TTCCGCTTTG TGTTGTTCCG TGCTTTGAAA 
     2761 TTTACCACAC GAAAAAAAAT TATACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     2821 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     2881 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     2941 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3001 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3061 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3121 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3181 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3241 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3301 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3361 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3421 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3481 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3541 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3601 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3661 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3721 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3781 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     3841 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     3901 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     3961 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4021 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4081 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4141 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4201 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4261 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4321 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4381 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4441 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4501 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4561 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4621 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4681 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4741 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4801 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     4861 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     4921 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     4981 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5041 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5101 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5161 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5221 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5281 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5341 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5401 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5461 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5521 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5581 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5641 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5701 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5761 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     5821 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     5881 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     5941 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6001 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6061 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6121 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6181 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6241 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6301 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6361 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6421 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6481 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6541 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6601 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//