LOCUS       RC212543                7335 bp    DNA     circular  SYN 
DEFINITION  RBM5 (Myc-DDK-tagged)-Human RNA binding motif protein 5 (RBM5), transcript variant 1
ACCESSION   NM_005778
VERSION     NM_005778.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3490..3581 
                     /label="Myc-DDK"  
     misc_feature    3611..4196 
                     /label="PolyA signal"  
     misc_feature    3668..3687 
                     /label="Restriction Enzyme"  
     misc_feature    4472..5144 
                     /label="ColE1"  
     misc_feature    5574..6368 
                     /label="Kan/Neo"  
     misc_feature    6403..6705 
                     /label="SV40 ori"  
     misc_feature    6791..7268 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3465..3472 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3465 
                     /label="RBM5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTCAGAC AAAAGAGTGA GTAGAACAGA GCGTAGTGGA AGATACGGTT 
     1081 CCATCATAGA CAGGGATGAC CGTGATGAGC GTGAATCCCG AAGCAGGCGG AGGGACTCAG 
     1141 ATTACAAAAG ATCTAGTGAT GATCGGAGGG GTGATAGATA TGATGACTAC CGAGACTATG 
     1201 ACAGTCCAGA GAGAGAGCGT GAAAGAAGGA ACAGTGACCG ATCCGAAGAT GGCTACCATT 
     1261 CAGATGGTGA CTATGGTGAG CACGACTATA GGCATGACAT CAGTGACGAG AGGGAGAGCA 
     1321 AGACCATCAT GCTGCGCGGC CTTCCCATCA CCATCACAGA GAGCGATATT CGAGAAATGA 
     1381 TGGAGTCCTT CGAAGGCCCT CAGCCTGCGG ATGTGAGGCT GATGAAGAGG AAAACAGGTG 
     1441 TAAGCCGTGG TTTCGCCTTC GTGGAGTTTT ATCACTTGCA AGATGCTACC AGCTGGATGG 
     1501 AAGCCAATCA GAAAAAGTTG GTGATTCAAG GAAAGCACAT TGCAATGCAT TATAGCAATC 
     1561 CCAGACCTAA GTTTGAAGAT TGGCTTTGTA ACAAGTGCTG CCTTAACAAT TTCAGGAAAA 
     1621 GACTAAAATG CTTCCGATGT GGAGCAGACA AGTTTGACTC TGAACAGGAA GTGCCTCCTG 
     1681 GAACCACAGA GTCGGTTCAG TCTGTGGATT ACTACTGTGA TACGATCATT CTTCGGAACA 
     1741 TAGCTCCGCA CACTGTGGTG GATTCCATCA TGACAGCACT GTCTCCTTAC GCGTCTTTAG 
     1801 CTGTCAATAA CATCCGCCTC ATAAAAGACA AACAGACCCA GCAGAACAGA GGCTTCGCAT 
     1861 TTGTGCAGCT GTCCTCTGCA ATGGATGCTT CTCAGCTGCT TCAGATATTA CAGAGTCTCC 
     1921 ATCCTCCTTT GAAAATTGAT GGCAAAACTA TTGGGGTTGA TTTTGCAAAA AGTGCCAGAA 
     1981 AAGACTTGGT CCTCTCAGAT GGTAACCGCG TCAGCGCCTT CTCTGTAGCT AGTACGGCTA 
     2041 TTGCTGCTGC TCAGTGGTCA TCCACCCAGT CTCAAAGTGG TGAAGGAGGC AGTGTTGACT 
     2101 ACAGTTATCT GCAACCAGGT CAAGATGGCT ATGCCCAATA TGCTCAGTAT TCACAGGATT 
     2161 ATCAGCAGTT TTATCAACAA CAAGCTGGAG GATTGGAATC TGATGCATCA TCTGCATCAG 
     2221 GCACAGCAGT GACCACCACC TCAGCGGCTG TAGTGTCCCA GAGTCCTCAG CTGTATAATC 
     2281 AAACCTCCAA TCCACCTGGC TCTCCGACTG AGGAAGCACA GCCTAGCACT AGCACAAGTA 
     2341 CACAGGCCCC AGCCGCTTCC CCTACTGGTG TAGTTCCTGG TACCAAATAT GCAGTACCTG 
     2401 ACACGTCCAC TTACCAGTAT GATGAATCTT CAGGATATTA CTATGATCCG ACAACAGGGC 
     2461 TCTATTATGA CCCCAACTCG CAATACTACT ATAATTCCTT GACCCAGCAG TACCTTTACT 
     2521 GGGATGGGGA AAAAGAGACC TACGTGCCAG CTGCAGAGTC TAGCTCCCAC CAGCAGTCGG 
     2581 GCCTGCCTCC TGCAAAAGAG GGGAAAGAGA AGAAGGAGAA ACCCAAGAGC AAAACAGCCC 
     2641 AGCAGATTGC CAAAGACATG GAACGCTGGG CTAAGAGTTT GAATAAGCAG AAAGAAAACT 
     2701 TTAAAAATAG CTTTCAGCCT GTCAATTCCT TGAGGGAAGA AGAAAGGAGA GAATCTGCTG 
     2761 CAGCAGACGC TGGCTTTGCT CTCTTTGAGA AGAAGGGAGC CTTAGCTGAA AGGCAGCAGC 
     2821 TCATCCCAGA ATTGGTGCGA AATGGAGATG AGGAGAATCC CCTCAAAAGG GGTCTGGTTG 
     2881 CTGCTTACAG TGGTGACAGT GACAATGAGG AGGAGCTGGT GGAGAGACTT GAGAGTGAGG 
     2941 AAGAGAAGCT AGCTGACTGG AAGAAGATGG CCTGTCTGCT CTGCCGGCGC CAGTTCCCGA 
     3001 ACAAAGATGC CCTAGTCAGG CACCAGCAAC TCTCAGACCT TCACAAGCAA AACATGGACA 
     3061 TCTACCGACG ATCCAGGCTG AGCGAGCAGG AGCTGGAAGC CTTGGAGCTA AGGGAGAGAG 
     3121 AGATGAAATA CCGAGACCGA GCTGCAGAAA GACGGGAGAA GTACGGCATT CCAGAACCTC 
     3181 CAGAGCCCAA GCGCAAGAAG CAGTTTGATG CCGGCACTGT GAATTACGAG CAACCCACCA 
     3241 AAGATGGCAT TGACCACAGT AACATTGGCA ACAAGATGCT GCAGGCCATG GGCTGGCGGG 
     3301 AAGGCTCTGG CTTGGGACGA AAGTGTCAAG GCATTACGGC TCCCATTGAG GCTCAAGTTC 
     3361 GGCTAAAGGG AGCTGGCCTA GGAGCCAAAG GCAGCGCATA TGGTTTGTCG GGCGCCGATT 
     3421 CCTACAAAGA TGCTGTCCGG AAAGCCATGT TTGCCCGGTT CACTGAGATG GAGAGCGGAC 
     3481 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3541 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3601 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3661 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3721 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3781 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3841 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3901 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3961 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4021 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4081 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4141 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4201 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4261 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4321 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4381 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4441 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4501 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4561 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4621 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4681 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4741 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4801 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4861 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4921 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4981 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5041 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5101 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5161 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5221 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5281 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5341 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5401 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5461 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5521 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5581 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5641 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5701 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5761 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5821 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5881 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5941 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6001 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6061 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6121 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6181 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6241 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6301 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6361 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6421 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6481 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6541 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6601 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6661 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6721 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6781 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6841 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6901 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6961 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7021 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7081 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7141 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7201 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7261 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7321 TTTAACGCGA ATTTT
//