LOCUS       RC212501L1                9102 bp    DNA     circular  SYN 
DEFINITION  ADAM12 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 12 (ADAM12), transcript variant 1
ACCESSION   NM_003474
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7393..7573 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8301..8960 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7732..8300 
                     /label="ColE1"  
     misc_feature    6268..6288 
                     /label="LR50 primer"  
     misc_feature    6065..6136 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6277..6868 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6047..6053 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6047 
                     /label="ADAM12"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGCGCGC CCGCTGCCCG TGTCCCCCGC 
     3361 CCGCGCCCTC CTGCTCGCCC TGGCCGGTGC TCTGCTCGCG CCCTGCGAGG CCCGAGGGGT 
     3421 GAGCTTATGG AACCAAGGAA GAGCTGATGA AGTTGTCAGT GCCTCTGTTG GGAGTGGGGA 
     3481 CCTCTGGATC CCAGTGAAGA GCTTCGACTC CAAGAATCAT CCAGAAGTGC TGAATATTCG 
     3541 ACTACAACGG GAAAGCAAAG AACTGATCAT AAATCTGGAA AGAAATGAAG GTCTCATTGC 
     3601 CAGCAGTTTC ACGGAAACCC ACTATCTGCA AGACGGTACT GATGTCTCCC TCGCTCGAAA 
     3661 TTACACGGTA ATTCTGGGTC ACTGTTACTA CCATGGACAT GTACGGGGAT ATTCTGATTC 
     3721 AGCAGTCAGT CTCAGCACGT GTTCTGGTCT CAGGGGACTT ATTGTGTTTG AAAATGAAAG 
     3781 CTATGTCTTA GAACCAATGA AAAGTGCAAC CAACAGATAC AAACTCTTCC CAGCGAAGAA 
     3841 GCTGAAAAGC GTCCGGGGAT CATGTGGATC ACATCACAAC ACACCAAACC TCGCTGCAAA 
     3901 GAATGTGTTT CCACCACCCT CTCAGACATG GGCAAGAAGG CATAAAAGAG AGACCCTCAA 
     3961 GGCAACTAAG TATGTGGAGC TGGTGATCGT GGCAGACAAC CGAGAGTTTC AGAGGCAAGG 
     4021 AAAAGATCTG GAAAAAGTTA AGCAGCGATT AATAGAGATT GCTAATCACG TTGACAAGTT 
     4081 TTACAGACCA CTGAACATTC GGATCGTGTT GGTAGGCGTG GAAGTGTGGA ATGACATGGA 
     4141 CAAATGCTCT GTAAGTCAGG ACCCATTCAC CAGCCTCCAT GAATTTCTGG ACTGGAGGAA 
     4201 GATGAAGCTT CTACCTCGCA AATCCCATGA CAATGCGCAG CTTGTCAGTG GGGTTTATTT 
     4261 CCAAGGGACC ACCATCGGCA TGGCCCCAAT CATGAGCATG TGCACGGCAG ACCAGTCTGG 
     4321 GGGAATTGTC ATGGACCATT CAGACAATCC CCTTGGTGCA GCCGTGACCC TGGCACATGA 
     4381 GCTGGGCCAC AATTTCGGGA TGAATCATGA CACACTGGAC AGGGGCTGTA GCTGTCAAAT 
     4441 GGCGGTTGAG AAAGGAGGCT GCATCATGAA CGCTTCCACC GGGTACCCAT TTCCCATGGT 
     4501 GTTCAGCAGT TGCAGCAGGA AGGACTTGGA GACCAGCCTG GAGAAAGGAA TGGGGGTGTG 
     4561 CCTGTTTAAC CTGCCGGAAG TCAGGGAGTC TTTCGGGGGC CAGAAGTGTG GGAACAGATT 
     4621 TGTGGAAGAA GGAGAGGAGT GTGACTGTGG GGAGCCAGAG GAATGTATGA ATCGCTGCTG 
     4681 CAATGCCACC ACCTGTACCC TGAAGCCGGA CGCTGTGTGC GCACATGGGC TGTGCTGTGA 
     4741 AGACTGCCAG CTGAAGCCTG CAGGAACAGC GTGCAGGGAC TCCAGCAACT CCTGTGACCT 
     4801 CCCAGAGTTC TGCACAGGGG CCAGCCCTCA CTGCCCAGCC AACGTGTACC TGCACGATGG 
     4861 GCACTCATGT CAGGATGTGG ACGGCTACTG CTACAATGGC ATCTGCCAGA CTCACGAGCA 
     4921 GCAGTGTGTC ACGCTCTGGG GACCAGGTGC TAAACCTGCC CCTGGGATCT GCTTTGAGAG 
     4981 AGTCAATTCT GCAGGTGATC CTTATGGCAA CTGTGGCAAA GTCTCGAAGA GTTCCTTTGC 
     5041 CAAATGCGAG ATGAGAGATG CTAAATGTGG AAAAATCCAG TGTCAAGGAG GTGCCAGCCG 
     5101 GCCAGTCATT GGTACCAATG CCGTTTCCAT AGAAACAAAC ATCCCCCTGC AGCAAGGAGG 
     5161 CCGGATTCTG TGCCGGGGGA CCCACGTGTA CTTGGGCGAT GACATGCCGG ACCCAGGGCT 
     5221 TGTGCTTGCA GGCACAAAGT GTGCAGATGG AAAAATCTGC CTGAATCGTC AATGTCAAAA 
     5281 TATTAGTGTC TTTGGGGTTC ACGAGTGTGC AATGCAGTGC CACGGCAGAG GGGTGTGCAA 
     5341 CAACAGGAAG AACTGCCACT GCGAGGCCCA CTGGGCACCT CCCTTCTGTG ACAAGTTTGG 
     5401 CTTTGGAGGA AGCACAGACA GCGGCCCCAT CCGGCAAGCA GATAACCAAG GTTTAACCAT 
     5461 AGGAATTCTG GTGACCATCC TGTGTCTTCT TGCTGCCGGA TTTGTGGTTT ATCTCAAAAG 
     5521 GAAGACCTTG ATACGACTGC TGTTTACAAA TAAGAAGACC ACCATTGAAA AACTAAGGTG 
     5581 TGTGCGCCCT TCCCGGCCAC CCCGTGGCTT CCAACCCTGT CAGGCTCACC TCGGCCACCT 
     5641 TGGAAAAGGC CTGATGAGGA AGCCGCCAGA TTCCTACCCA CCGAAGGACA ATCCCAGGAG 
     5701 ATTGCTGCAG TGTCAGAATG TTGACATCAG CAGACCCCTC AACGGCCTGA ATGTCCCTCA 
     5761 GCCCCAGTCA ACTCAGCGAG TGCTTCCTCC CCTCCACCGG GCCCCACGTG CACCTAGCGT 
     5821 CCCTGCCAGA CCCCTGCCAG CCAAGCCTGC ACTTAGGCAG GCCCAGGGGA CCTGTAAGCC 
     5881 AAACCCCCCT CAGAAGCCTC TGCCTGCAGA TCCTCTGGCC AGAACAACTC GGCTCACTCA 
     5941 TGCCTTGGCC AGGACCCCAG GACAATGGGA GACTGGGCTC CGCCTGGCAC CCCTCAGACC 
     6001 TGCTCCACAA TATCCACACC AAGTGCCCAG ATCCACCCAC ACCGCCTATA TTAAGACGCG 
     6061 TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT 
     6121 GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCTGT ACAAGTAGGA 
     6181 TTCGTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ACATGTTTAA 
     6241 GGGTTCCGGT TCCACTAGGT ACAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT 
     6301 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 
     6361 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 
     6421 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 
     6481 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 
     6541 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 
     6601 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 
     6661 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 
     6721 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 
     6781 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 
     6841 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGATCGAGA 
     6901 CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC 
     6961 CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT 
     7021 AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG 
     7081 ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA 
     7141 CACACAAGGC TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGA TCAGATATCC 
     7201 ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC 
     7261 CAATGAAGGA GAGAACACCC GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC 
     7321 GGAGAGAGAA GTATTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACATGGCCCG 
     7381 AGAGCTGCAT CCGGACTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 
     7441 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 
     7501 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 
     7561 GTCAGTGTGG AAAATCTCTA GCAGCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     7621 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     7681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     7741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     7801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     7861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     7921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     7981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     8041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     8101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     8161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     8221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     8281 AAACTCACGT TAAGGGATTT TGGTCATGAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC 
     8341 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC 
     8401 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA 
     8461 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC 
     8521 CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG 
     8581 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG 
     8641 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA 
     8701 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA 
     8761 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT 
     8821 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT 
     8881 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG 
     8941 GTGGTATATC CAGTGATTTT TTTCTCCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     9001 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     9061 ATAGGGGTCC CGCGCACATT TCCCCGAAAA GTGCCACCTG AC
//