LOCUS       RC212488                7235 bp    DNA     circular  SYN 
DEFINITION  ZFYVE20 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 20 (ZFYVE20)
ACCESSION   NM_022340
VERSION     NM_022340.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3392..3483 
                     /label="Myc-DDK"  
     misc_feature    3513..4098 
                     /label="PolyA signal"  
     misc_feature    3570..3589 
                     /label="Restriction Enzyme"  
     misc_feature    4374..5046 
                     /label="ColE1"  
     misc_feature    5476..6270 
                     /label="Kan/Neo"  
     misc_feature    6305..6607 
                     /label="SV40 ori"  
     misc_feature    6693..7170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3374..3380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3374 
                     /label="RBSN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTCTG GACGACCCAG GGGAAGTGAG GGAGGGCTTC CTCTGCCCTC 
     1081 TGTGCCTGAA GGATCTGCAG TCTTTCTATC AGCTTCACTC ACATTACGAG GAAGAACACT 
     1141 CAGGGGAAGA CCGTGATGTC AAAGGGCAAA TTAAAAGTCT TGTCCAGAAG GCTAAAAAAG 
     1201 CAAAGGACAG GTTGTTGAAA CGAGAAGGGG ATGATCGAGC AGAGTCAGGG ACCCAAGGAT 
     1261 ATGAGTCTTT CAGCTATGGA GGGGTTGATC CTTACATGTG GGAACCCCAG GAGCTTGGTG 
     1321 CTGTGAGAAG CCATCTTTCC GACTTCAAAA AACACCGAGC TGCTAGAATT GACCACTATG 
     1381 TTGTGGAAGT CAATAAACTA ATAATCAGGT TAGAGAAGCT CACTGCATTT GACAGAACAA 
     1441 ATACTGAGTC TGCAAAGATT CGAGCAATAG AAAAGTCTGT GGTGCCTTGG GTCAACGACC 
     1501 AGGATGTCCC TTTCTGTCCA GACTGTGGGA ATAAGTTCAG CATCCGGAAC CGCCGCCACC 
     1561 ACTGCCGCCT CTGCGGGTCT ATTATGTGCA AGAAGTGTAT GGAGCTCATC AGCCTTCCCT 
     1621 TGGCAAACAA GCTCACCAGT GCCAGCAAGG AGTCCCTGAG CACCCACACC AGCCCCAGCC 
     1681 AGTCACCCAA CAGTGTCCAT GGCTCCCGCC GAGGCAGCAT CAGCAGCATG AGCAGTGTCA 
     1741 GCTCGGTCCT GGATGAGAAG GACGATGACC GGATCCGCTG CTGTACACAC TGCAAGGACA 
     1801 CGCTGCTCAA GAGAGAGCAG CAGATTGATG AGAAGGAGCA CACACCTGAC ATCGTGAAGC 
     1861 TCTACGAGAA ATTACGACTT TGCATGGAGA AAGTTGACCA GAAAGCTCCA GAATACATCA 
     1921 GGATGGCAGC ATCATTAAAT GCTGGGGAGA CAACCTACAG TCTGGAACAT GCCAGTGACC 
     1981 TTCGAGTGGA AGTGCAGAAA GTGTATGAGC TGATAGACGC TTTAAGTAAG AAGATCTTAA 
     2041 CCTTGGGCTT GAACCAGGAC CCTCCACCAC ATCCAAGCAA TTTGCGGCTG CAGAGAATGA 
     2101 TCAGATACTC AGCTACACTT TTTGTGCAGG AAAAGTTGCT TGGTTTGATG TCACTGCCAA 
     2161 CCAAAGAACA GTTTGAGGAA CTGAAAAAGA AAAGGAAGGA GGAAATGGAG AGGAAGAGGG 
     2221 CCGTGGAGAG ACAAGCTGCC CTGGAGTCCC AGCGAAGGCT TGAGGAAAGG CAGAGTGGCC 
     2281 TGGCTTCTCG AGCGGCCAAC GGGGAGGTGG CATCTCTCCG CAGGGGCCCT GCCCCCTTGA 
     2341 GAAAGGCTGA GGGCTGGCTC CCACTGTCAG GAGGTCAGGG GCAGAGTGAG GACTCAGACC 
     2401 CGCTCCTCCA GCAGATCCAC AACATCACAT CATTCATCAG GCAGGCCAAG GCCGCGGGCC 
     2461 GCATGGATGA AGTGCGCACT CTGCAGGAGA ACCTGCGGCA GCTGCAGGAC GAGTATGACC 
     2521 AGCAGCAGAC AGAGAAGGCC ATCGAGCTGT CCCGGAGGCA GGCTGAGGAG GAGGACCTGC 
     2581 AGCGGGAACA GCTGCAGATG TTGCGTGAAC GGGAGTTGGA ACGAGAAAGG GAGCAGTTTC 
     2641 GGGTGGCATC CCTGCACACA CGGACTCGGT CCCTGGACTT CAGAGAAATC GGCCCTTTTC 
     2701 AGCTGGAGCC CAGCAGAGAG CCTCGCACCC ACCTTGCTTA TGCTTTGGAT CTAGGCTCTT 
     2761 CCCCAGTTCC AAGCAGCACA GCTCCCAAGA CCCCTTCACT TAGCTCAACT CAACCCACCA 
     2821 GAGTGTGGTC TGGGCCCCCA GCCGTTGGCC AGGAGCGCTT ACCCCAGAGC AGCATGCCAC 
     2881 AGCAACATGA GGGGCCCTCC TTAAACCCCT TTGATGAGGA AGACCTCTCC AGCCCCATGG 
     2941 AAGAGGCCAC TACTGGTCCT CCTGCTGCAG GGGTTTCCTT AGACCCTTCA GCCCGCATCC 
     3001 TGAAAGAGTA CAATCCTTTC GAGGAAGAGG ACGAGGAGGA GGAAGCAGTG GCAGGGAATC 
     3061 CATTCATTCA GCCAGACAGC CCAGCTCCTA ACCCCTTCAG TGAGGAAGAC GAACATCCCC 
     3121 AGCAGAGGCT CTCAAGCCCT CTGGTTCCTG GTAACCCCTT TGAGGAACCC ACCTGTATCA 
     3181 ACCCCTTTGA GATGGACAGT GACAGTGGGC CAGAGGCTGA GGAGCCCATA GAGGAAGAGC 
     3241 TCCTCCTGCA GCAGATCGAT AACATCAAGG CATACATCTT TGATGCCAAG CAGTGCGGCC 
     3301 GCCTGGATGA GGTAGAGGTG CTGACAGAGA ATCTGCGGGA GCTGAAGCAC ACCCTGGCCA 
     3361 AGCAGAAGGG GGGCACTGAC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     3421 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     3481 GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT 
     3541 CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA 
     3601 TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA 
     3661 GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT 
     3721 ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT 
     3781 GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA 
     3841 CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC 
     3901 TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT 
     3961 TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC 
     4021 AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA 
     4081 GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT 
     4141 TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 
     4201 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 
     4261 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 
     4321 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 
     4381 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 
     4441 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 
     4501 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 
     4561 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 
     4621 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 
     4681 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 
     4741 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 
     4801 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 
     4861 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 
     4921 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 
     4981 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG 
     5041 GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT 
     5101 GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT 
     5161 GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA 
     5221 ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT 
     5281 GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA 
     5341 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT 
     5401 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG 
     5461 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA 
     5521 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC 
     5581 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA 
     5641 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG 
     5701 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA 
     5761 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT 
     5821 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT 
     5881 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG 
     5941 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA 
     6001 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC 
     6061 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG 
     6121 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG 
     6181 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG 
     6241 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG 
     6301 TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG 
     6361 AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA 
     6421 TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC 
     6481 GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG 
     6541 CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     6601 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC 
     6661 TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     6721 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     6781 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     6841 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     6901 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     6961 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     7021 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     7081 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     7141 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     7201 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//