LOCUS       RC212391                8292 bp    DNA     circular  SYN 
DEFINITION  MSH3 (Myc-DDK-tagged)-Human mutS homolog 3 (E. coli) (MSH3)
ACCESSION   NM_002439
VERSION     NM_002439.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="MSH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCGCCGG AAGCCTGCGT CGGGCGGCCT CGCTGCCTCC AGCTCAGCCC 
     1081 CTGCGAGGCA AGCGGTTTTG AGCCGATTCT TCCAGTCTAC GGGAAGCCTG AAATCCACCT 
     1141 CCTCCTCCAC AGGTGCAGCC GACCAGGTGG ACCCTGGCGC TGCAGCGGCT GCAGCGGCCG 
     1201 CAGCGGCCGC AGCGCCCCCA GCGCCCCCAG CTCCCGCCTT CCCGCCCCAG CTGCCGCCGC 
     1261 ACATAGCTAC AGAAATTGAC AGAAGAAAGA AGAGACCATT GGAAAATGAT GGGCCTGTTA 
     1321 AAAAGAAAGT AAAGAAAGTC CAACAAAAGG AAGGAGGAAG TGATCTGGGA ATGTCTGGCA 
     1381 ACTCTGAGCC AAAGAAATGT CTGAGGACCA GGAATGTTTC AAAGTCTCTG GAAAAATTGA 
     1441 AAGAATTCTG CTGCGATTCT GCCCTTCCTC AAAGTAGAGT CCAGACAGAA TCTCTGCAGG 
     1501 AGAGATTTGC AGTTCTGCCA AAATGTACTG ATTTTGATGA TATCAGTCTT CTACACGCAA 
     1561 AGAATGCAGT TTCTTCTGAA GATTCGAAAC GTCAAATTAA TCAAAAGGAC ACAACACTTT 
     1621 TTGATCTCAG TCAGTTTGGA TCATCAAATA CAAGTCATGA AAATTTACAG AAAACTGCTT 
     1681 CCAAATCAGC TAACAAACGG TCCAAAAGCA TCTATACGCC GCTAGAATTA CAATACATAG 
     1741 AAATGAAGCA GCAGCACAAA GATGCAGTTT TGTGTGTGGA ATGTGGATAT AAGTATAGAT 
     1801 TCTTTGGGGA AGATGCAGAG ATTGCAGCCC GAGAGCTCAA TATTTATTGC CATTTAGATC 
     1861 ACAACTTTAT GACAGCAAGT ATACCTACTC ACAGACTGTT TGTTCATGTA CGCCGCCTGG 
     1921 TGGCAAAAGG ATATAAGGTG GGAGTTGTGA AGCAAACTGA AACTGCAGCA TTAAAGGCCA 
     1981 TTGGAGACAA CAGAAGTTCA CTCTTTTCCC GGAAATTGAC TGCCCTTTAT ACAAAATCTA 
     2041 CACTTATTGG AGAAGATGTG AATCCCCTAA TCAAGCTGGA TGATGCTGTA AATGTTGATG 
     2101 AGATAATGAC TGATACTTCT ACCAGCTATC TTCTGTGCAT CTCTGAAAAT AAGGAAAATG 
     2161 TTAGGGACAA AAAAAAGGGC AACATTTTTA TTGGCATTGT GGGAGTGCAG CCTGCCACAG 
     2221 GCGAGGTTGT GTTTGATAGT TTCCAGGACT CTGCTTCTCG TTCAGAGCTA GAAACCCGGA 
     2281 TGTCAAGCCT GCAGCCAGTA GAGCTGCTGC TTCCTTCGGC CTTGTCCGAG CAAACAGAGG 
     2341 CGCTCATCCA CAGAGCCACA TCTGTTAGTG TGCAGGATGA CAGAATTCGA GTCGAAAGGA 
     2401 TGGATAACAT TTATTTTGAA TACAGCCATG CTTTCCAGGC AGTTACAGAG TTTTATGCAA 
     2461 AAGATACAGT TGACATCAAA GGTTCTCAAA TTATTTCTGG CATTGTTAAC TTAGAGAAGC 
     2521 CTGTGATTTG CTCTTTGGCT GCCATCATAA AATACCTCAA AGAATTCAAC TTGGAAAAGA 
     2581 TGCTCTCCAA ACCTGAGAAT TTTAAACAGC TATCAAGTAA AATGGAATTT ATGACAATTA 
     2641 ATGGAACAAC ATTAAGGAAT CTGGAAATCC TACAGAATCA GACTGATATG AAAACCAAAG 
     2701 GAAGTTTGCT GTGGGTTTTA GACCACACTA AAACTTCATT TGGGAGACGG AAGTTAAAGA 
     2761 AGTGGGTGAC CCAGCCACTC CTTAAATTAA GGGAAATAAA TGCCCGGCTT GATGCTGTAT 
     2821 CGGAAGTTCT CCATTCAGAA TCTAGTGTGT TTGGTCAGAT AGAAAATCAT CTACGTAAAT 
     2881 TGCCCGACAT AGAGAGGGGA CTCTGTAGCA TTTATCACAA AAAATGTTCT ACCCAAGAGT 
     2941 TCTTCTTGAT TGTCAAAACT TTATATCACC TAAAGTCAGA ATTTCAAGCA ATAATACCTG 
     3001 CTGTTAATTC CCACATTCAG TCAGACTTGC TCCGGACCGT TATTTTAGAA ATTCCTGAAC 
     3061 TCCTCAGTCC AGTGGAGCAT TACTTAAAGA TACTCAATGA ACAAGCTGCC AAAGTTGGGG 
     3121 ATAAAACTGA ATTATTTAAA GACCTTTCTG ACTTCCCTTT AATAAAAAAG AGGAAGGATG 
     3181 AAATTCAAGG TGTTATTGAC GAGATCCGAA TGCATTTGCA AGAAATACGA AAAATACTAA 
     3241 AAAATCCTTC TGCACAATAT GTGACAGTAT CAGGACAGGA GTTTATGATA GAAATAAAGA 
     3301 ACTCTGCTGT ATCTTGTATA CCAACTGATT GGGTAAAGGT TGGAAGCACA AAAGCTGTGA 
     3361 GCCGCTTTCA CTCTCCTTTT ATTGTAGAAA ATTACAGACA TCTGAATCAG CTCCGGGAGC 
     3421 AGCTAGTCCT TGACTGCAGT GCTGAATGGC TTGATTTTCT AGAGAAATTC AGTGAACATT 
     3481 ATCACTCCTT GTGTAAAGCA GTGCATCACC TAGCAACTGT TGACTGCATT TTCTCCCTGG 
     3541 CCAAGGTCGC TAAGCAAGGA GATTACTGCA GACCAACTGT ACAAGAAGAA AGAAAAATTG 
     3601 TAATAAAAAA TGGAAGGCAC CCTGTGATTG ATGTGTTGCT GGGAGAACAG GATCAATATG 
     3661 TCCCAAATAA TACAGATTTA TCAGAGGACT CAGAGAGAGT AATGATAATT ACCGGACCAA 
     3721 ACATGGGTGG AAAGAGCTCC TACATAAAAC AAGTTGCATT GATTACCATC ATGGCTCAGA 
     3781 TTGGCTCCTA TGTTCCTGCA GAAGAAGCGA CAATTGGGAT TGTGGATGGC ATTTTCACAA 
     3841 GGATGGGTGC TGCAGACAAT ATATATAAAG GACAGAGTAC ATTTATGGAA GAACTGACTG 
     3901 ACACAGCAGA AATAATCAGA AAAGCAACAT CACAGTCCTT GGTTATCTTG GATGAACTAG 
     3961 GAAGAGGGAC GAGCACTCAT GATGGAATTG CCATTGCCTA TGCTACACTT GAGTATTTCA 
     4021 TCAGAGATGT GAAATCCTTA ACCCTGTTTG TCACCCATTA TCCGCCAGTT TGTGAACTAG 
     4081 AAAAAAATTA CTCACACCAG GTGGGGAATT ACCACATGGG ATTCTTGGTC AGTGAGGATG 
     4141 AAAGCAAACT GGATCCAGGC GCAGCAGAAC AAGTCCCTGA TTTTGTCACC TTCCTTTACC 
     4201 AAATAACTAG AGGAATTGCA GCAAGGAGTT ATGGATTAAA TGTGGCTAAA CTAGCAGATG 
     4261 TTCCTGGAGA AATTTTGAAG AAAGCAGCTC ACAAGTCAAA AGAGCTGGAA GGATTAATAA 
     4321 ATACGAAAAG AAAGAGACTC AAGTATTTTG CAAAGTTATG GACGATGCAT AATGCACAAG 
     4381 ACCTGCAGAA GTGGACAGAG GAGTTCAACA TGGAAGAAAC ACAGACTTCT CTTCTTCATA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//