LOCUS       RC212366                8745 bp    DNA     circular  SYN 
DEFINITION  SMC4 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 4 (SMC4), transcript variant 2
ACCESSION   NM_001002800
VERSION     NM_001002800.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="SMC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGTAAA GGCACCCAGC CCTCCACTGC CCGGCGCAGA GAGGAAGGGC 
     1081 CGCCGCCGCC GTCCCCTGAC GGCGCCAGCA GCGACGCGGA GCCTGAGCCG CCGTCCGGCC 
     1141 GCACGGAGAG CCCAGCCACC GCCGCAGAGA CTGCAAGTGA GGAACTTGAT AATAGAAGTT 
     1201 TAGAAGAGAT TTTGAACAGC ATTCCTCCTC CCCCGCCTCC AGCAATGACC AATGAAGCTG 
     1261 GAGCTCCTCG GCTTATGATA ACTCATATTG TAAACCAGAA CTTCAAATCC TATGCTGGGG 
     1321 AGAAAATTCT GGGACCTTTC CATAAGCGCT TTTCCTGTAT TATCGGGCCA AATGGCAGTG 
     1381 GCAAATCCAA TGTTATTGAT TCTATGCTTT TTGTGTTTGG CTATCGAGCA CAAAAAATAA 
     1441 GATCTAAAAA ACTCTCAGTA TTAATACATA ATTCTGATGA ACACAAGGAC ATTCAGAGTT 
     1501 GTACAGTAGA AGTTCATTTT CAAAAGATAA TTGATAAGGA AGGGGATGAT TATGAAGTCA 
     1561 TTCCTAACAG TAATTTCTAT GTATCCAGAA CGGCCTGCAG AGATAATACT TCTGTCTATC 
     1621 ACATAAGTGG AAAGAAAAAG ACATTTAAGG ATGTTGGAAA TCTTCTTCGA AGCCATGGAA 
     1681 TTGACTTGGA CCATAATAGA TTTTTAATTT TACAGGGTGA AGTTGAACAA ATTGCTATGA 
     1741 TGAAACCAAA AGGCCAGACT GAACACGATG AGGGTATGCT TGAATATTTA GAAGATATAA 
     1801 TTGGTTGTGG ACGGCTAAAT GAACCTATTA AAGTCTTGTG TCGGAGAGTT GAAATATTAA 
     1861 ATGAACACAG AGGAGAGAAG TTAAACAGGG TAAAGATGGT GGAAAAGGAA AAGGATGCCT 
     1921 TAGAAGGAGA GAAAAACATA GCTATCGAAT TTCTTACCTT GGAAAATGAA ATATTTAGAA 
     1981 AAAAGAATCA TGTTTGTCAA TATTATATTT ATGAGTTGCA GAAACGAATT GCTGAAATGG 
     2041 AAACTCAAAA GGAAAAAATT CATGAAGATA CCAAAGAAAT TAATGAGAAG AGCAATATAC 
     2101 TATCAAATGA AATGAAAGCT AAGAATAAAG ATGTAAAAGA TACAGAAAAG AAACTGAATA 
     2161 AAATTACAAA ATTTATTGAG GAGAATAAAG AAAAATTTAC ACAGCTAGAT TTGGAAGATG 
     2221 TTCAAGTTAG AGAAAAGTTA AAACATGCCA CGAGTAAAGC CAAAAAACTG GAGAAACAAC 
     2281 TTCAAAAAGA TAAAGAAAAG GTTGAAGAAT TTAAAAGTAT ACCTGCCAAG AGTAACAATA 
     2341 TCATTAATGA AACAACAACC AGAAACAATG CCCTCGAGAA GGAAAAAGAG AAAGAAGAAA 
     2401 AAAAATTAAA GGAAGTTATG GATAGCCTTA AACAGGAAAC ACAAGGGCTT CAGAAAGAAA 
     2461 AAGAAAGTCG AGAGAAAGAA CTTATGGGTT TCAGCAAATC GGTAAATGAA GCACGTTCAA 
     2521 AGATGGATGT AGCCCAGTCA GAACTTGATA TCTATCTCAG TCGTCATAAT ACTGCAGTGT 
     2581 CTCAATTAAC TAAGGCTAAG GAAGCTCTAA TTGCAGCTTC TGAGACTCTC AAAGAAAGGA 
     2641 AAGCTGCAAT CAGAGATATA GAAGGAAAAC TCCCTCAAAC TGAACAAGAA TTAAAGGAGA 
     2701 AAGAAAAAGA ACTTCAAAAA CTTACACAAG AAGAAACAAA CTTTAAAAGT TTGGTTCATG 
     2761 ATCTCTTTCA AAAAGTTGAA GAAGCAAAGA GCTCATTAGC AATGAATCGA AGTAGGGGGA 
     2821 AAGTCCTTGA TGCAATAATT CAAGAAAAAA AATCTGGCAG GATTCCAGGA ATATATGGAA 
     2881 GATTGGGGGA CTTAGGAGCC ATTGATGAAA AATACGACGT GGCTATATCA TCCTGTTGTC 
     2941 ATGCACTGGA CTACATTGTT GTTGATTCTA TTGATATAGC CCAAGAATGT GTAAACTTCC 
     3001 TTAAAAGACA AAATATTGGA GTTGCAACCT TTATAGGTTT AGATAAGATG GCTGTATGGG 
     3061 CGAAAAAGAT GACCGAAATT CAAACTCCTG AAAATACTCC TCGTTTATTT GATTTAGTAA 
     3121 AAGTAAAAGA TGAGAAAATT CGCCAAGCTT TTTATTTTGC TTTACGAGAT ACCTTAGTAG 
     3181 CTGACAACTT GGATCAAGCC ACAAGAGTAG CATATCAAAA AGATAGAAGA TGGAGAGTGG 
     3241 TAACTTTACA GGGACAAATC ATAGAACAGT CAGGTACAAT GACTGGTGGT GGAAGCAAAG 
     3301 TAATGAAAGG AAGAATGGGT TCCTCACTTG TTATTGAAAT CTCTGAAGAA GAGGTAAACA 
     3361 AAATGGAATC ACAGTTGCAA AACGACTCTA AAAAAGCAAT GCAAATCCAA GAACAGAAAG 
     3421 TACAACTTGA AGAAAGAGTA GTTAAGTTAC GGCATAGTGA ACGAGAAATG AGGAACACAC 
     3481 TAGAAAAATT TACTGCAAGC ATCCAGCGTT TAATAGAGCA AGAAGAATAT TTGAATGTCC 
     3541 AAGTTAAGGA ACTTGAAGCT AATGTACTTG CTACAGCCCC TGACAAAAAA AAGCAGAAAT 
     3601 TGCTAGAAGA AAACGTTAGT GCTTTCAAAA CAGAATATGA TGCTGTGGCT GAGAAAGCTG 
     3661 GTAAAGTAGA AGCTGAGGTT AAACGCTTAC ACAATACCAT CGTAGAAATC AATAATCATA 
     3721 AACTCAAGGC CCAACAAGAC AAACTTGATA AAATAAATAA GCAATTAGAT GAATGTGCTT 
     3781 CTGCTATTAC TAAAGCCCAA GTAGCAATCA AGACTGCTGA CAGAAACCTT CAAAAGGCAC 
     3841 AAGACTCTGT CTTGCGTACA GAGAAAGAAA TAAAAGATAC TGAGAAAGAG GTGGATGACC 
     3901 TAACAGCAGA GCTGAAAAGT CTTGAGGACA AAGCAGCAGA GGTCGTAAAG AATACAAATG 
     3961 CTGCAGAGGA ATCCTTACCA GAGATCCAGA AAGAACATCG CAATCTGCTT CAAGAATTAA 
     4021 AAGTTATTCA AGAAAATGAA CATGCTCTTC AAAAAGATGC ACTTAGTATT AAGTTGAAAC 
     4081 TTGAACAAAT AGATGGTCAC ATTGCTGAAC ATAATTCTAA AATAAAATAT TGGCACAAAG 
     4141 AGATTTCAAA AATATCACTG CATCCTATAG AAGATAATCC TATTGAAGAG ATTTCGGTTC 
     4201 TAAGCCCAGA GGATCTTGAA GCGATCAAGA ATCCAGATTC TATAACAAAT CAAATTGCAC 
     4261 TTTTGGAAGC CCGGTGTCAT GAAATGAAAC CAAACCTCGG TGCCATCGCA GAGTATAAAA 
     4321 AGAAGGAAGA ATTGTATTTG CAACGGGTAG CAGAATTGGA CAAAATTACT TATGAAAGAG 
     4381 ACAGTTTTAG ACAGGCATAT GAAGATCTTC GGAAACAAAG GCTTAATGAA TTTATGGCAG 
     4441 GTTTTTATAT AATAACAAAT AAATTAAAGG AAAATTACCA AATGCTTACT TTGGGAGGGG 
     4501 ACGCCGAACT CGAGCTTGTA GACAGCTTGG ATCCTTTCTC TGAAGGAATC ATGTTCAGTG 
     4561 TTCGACCACC TAAGAAAAGT TGGAAAAAGA TCTTCAACCT TTCGGGAGGA GAGAAAACAC 
     4621 TTAGTTCATT GGCTTTAGTA TTTGCTCTTC ACCACTACAA GCCCACTCCC CTTTACTTCA 
     4681 TGGATGAGAT TGATGCAGCC CTTGATTTTA AAAATGTGTC CATTGTTGCA TTTTATATAT 
     4741 ATGAACAAAC AAAAAATGCA CAGTTCATAA TAATTTCTCT TCGAAATAAT ATGTTTGAGA 
     4801 TTTCGGATAG ACTTATTGGA ATTTACAAGA CATACAACAT AACAAAAAGT GTTGCTGTAA 
     4861 ATCCAAAAGA AATTGCATCT AAGGGACTTT GTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//