LOCUS       RC212294                8235 bp    DNA     circular  SYN 
DEFINITION  DSG2 (Myc-DDK-tagged)-Human desmoglein 2 (DSG2)
ACCESSION   NM_001943
VERSION     NM_001943.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4392..4483 
                     /label="Myc-DDK"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    4570..4589 
                     /label="Restriction Enzyme"  
     misc_feature    5374..6046 
                     /label="ColE1"  
     misc_feature    6476..7270 
                     /label="Kan/Neo"  
     misc_feature    7305..7607 
                     /label="SV40 ori"  
     misc_feature    7693..8170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="DSG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGGAGC CCGGGACGCG CGTACGCCCT GCTGCTTCTC CTGATCTGCT 
     1081 TTAACGTTGG AAGTGGACTT CACTTACAGG TCTTAAGCAC AAGAAATGAA AATAAGCTGC 
     1141 TTCCTAAACA TCCTCATTTA GTGCGGCAAA AGCGCGCCTG GATCACCGCC CCCGTGGCTC 
     1201 TTCGGGAGGG AGAGGATCTG TCCAAGAAGA ATCCAATTGC CAAGATACAT TCTGATCTTG 
     1261 CAGAAGAAAG AGGACTCAAA ATTACTTACA AATACACTGG AAAAGGGATT ACAGAGCCAC 
     1321 CTTTTGGTAT ATTTGTCTTT AACAAAGATA CTGGAGAACT GAATGTTACC AGCATTCTTG 
     1381 ATCGAGAAGA AACACCATTT TTTCTGCTAA CAGGTTACGC TTTGGATGCA AGAGGAAACA 
     1441 ATGTAGAGAA ACCCTTAGAG CTACGCATTA AGGTTCTTGA TATCAATGAC AACGAACCAG 
     1501 TGTTCACACA GGATGTCTTT GTTGGGTCTG TTGAAGAGTT GAGTGCAGCA CATACTCTTG 
     1561 TGATGAAAAT CAATGCAACA GATGCAGATG AGCCCAATAC CCTGAATTCG AAAATTTCCT 
     1621 ATAGAATCGT ATCTCTGGAG CCTGCTTATC CTCCAGTGTT CTACCTAAAT AAAGATACAG 
     1681 GAGAGATTTA TACAACCAGT GTTACCTTGG ACAGAGAGGA ACACAGCAGC TACACTTTGA 
     1741 CAGTAGAAGC AAGAGATGGC AATGGAGAAG TTACAGACAA ACCTGTAAAA CAAGCTCAAG 
     1801 TTCAGATTCG TATTTTGGAT GTCAATGACA ATATACCTGT AGTAGAAAAT AAAGTGCTTG 
     1861 AAGGGATGGT TGAAGAAAAT CAAGTCAACG TAGAAGTTAC GCGCATAAAA GTGTTCGATG 
     1921 CAGATGAAAT AGGTTCTGAT AATTGGCTGG CAAATTTTAC ATTTGCATCA GGAAATGAAG 
     1981 GAGGTTATTT CCACATAGAA ACAGATGCTC AAACTAACGA AGGAATTGTG ACCCTTATTA 
     2041 AGGAAGTAGA TTATGAAGAA ATGAAGAATC TTGACTTCAG TGTTATTGTC GCTAATAAAG 
     2101 CAGCTTTTCA CAAGTCGATT AGGAGTAAAT ACAAGCCTAC ACCCATTCCC ATCAAGGTCA 
     2161 AAGTGAAAAA TGTGAAAGAA GGCATTCATT TTAAAAGCAG CGTCATCTCA ATTTATGTTA 
     2221 GCGAGAGCAT GGATAGATCA AGCAAAGGCC AAATAATTGG AAATTTTCAA GCTTTTGATG 
     2281 AGGACACTGG ACTACCAGCC CATGCAAGAT ATGTAAAATT AGAAGATAGA GATAATTGGA 
     2341 TCTCTGTGGA TTCTGTCACA TCTGAAATTA AACTTGCAAA ACTTCCTGAT TTTGAATCTA 
     2401 GATATGTTCA AAATGGCACA TACACTGTAA AGATTGTGGC CATATCAGAA GATTATCCTA 
     2461 GAAAAACCAT CACTGGCACA GTCCTTATCA ATGTTGAAGA CATCAACGAC AACTGTCCCA 
     2521 CACTGATAGA GCCTGTGCAG ACAATCTGTC ACGATGCAGA GTATGTGAAT GTTACTGCAG 
     2581 AGGACCTGGA TGGACACCCA AACAGTGGCC CTTTCAGTTT CTCCGTCATT GACAAACCAC 
     2641 CTGGCATGGC AGAAAAATGG AAAATAGCAC GCCAAGAAAG TACCAGTGTG CTGCTGCAAC 
     2701 AAAGTGAGAA AAAGCTTGGG AGAAGTGAAA TTCAGTTCCT GATTTCAGAC AATCAGGGTT 
     2761 TTAGTTGTCC TGAAAAGCAG GTCCTTACAC TCACAGTTTG TGAGTGTCTG CATGGCAGCG 
     2821 GCTGCAGGGA AGCACAGCAT GACTCCTATG TGGGCCTGGG ACCCGCAGCA ATTGCGCTCA 
     2881 TGATTTTGGC CTTTCTGCTC CTGCTATTGG TACCACTTTT ACTGCTGATG TGCCATTGCG 
     2941 GAAAGGGCGC CAAAGGCTTT ACCCCCATAC CTGGCACCAT AGAGATGCTG CATCCTTGGA 
     3001 ATAATGAAGG AGCACCACCT GAAGACAAGG TGGTGCCATC ATTTCTGCCA GTGGATCAAG 
     3061 GGGGCAGTCT AGTAGGAAGA AATGGAGTAG GAGGTATGGC CAAGGAAGCC ACGATGAAAG 
     3121 GAAGTAGCTC TGCTTCCATT GTCAAAGGGC AACATGAGAT GTCCGAGATG GATGGAAGGT 
     3181 GGGAAGAACA CAGAAGCCTG CTTTCTGGTA GAGCTACCCA GTTTACAGGG GCCACAGGCG 
     3241 CTATCATGAC CACTGAAACC ACGAAGACCG CAAGGGCCAC AGGGGCTTCC AGAGACATGG 
     3301 CCGGAGCTCA GGCAGCTGCT GTTGCACTGA ACGAAGAATT CTTAAGAAAT TATTTCACTG 
     3361 ATAAAGCGGC CTCTTACACT GAGGAAGATG AAAATCACAC AGCCAAAGAT TGCCTTCTGG 
     3421 TTTATTCTCA GGAAGAAACT GAATCGCTGA ATGCTTCTAT TGGTTGTTGC AGTTTTATTG 
     3481 AAGGAGAGCT AGATGACCGC TTCTTAGATG ATTTGGGACT TAAATTCAAG ACACTAGCTG 
     3541 AAGTTTGCCT GGGTCAAAAA ATAGATATAA ATAAGGAAAT TGAGCAGAGA CAAAAACCTG 
     3601 CCACAGAAAC AAGTATGAAC ACAGCTTCAC ATTCACTCTG TGAGCAAACT ATGGTTAATT 
     3661 CAGAGAATAC CTACTCCTCT GGCAGTAGCT TCCCAGTTCC AAAATCTTTG CAAGAAGCCA 
     3721 ATGCAGAGAA AGTAACTCAG GAAATAGTCA CTGAAAGATC TGTGTCTTCT AGGCAGGCGC 
     3781 AAAAGGTAGC TACACCTCTT CCTGACCCAA TGGCTTCTAG AAATGTGATA GCAACAGAAA 
     3841 CTTCCTATGT CACAGGGTCC ACTATGCCAC CAACCACTGT GATCCTGGGT CCTAGCCAGC 
     3901 CACAGAGCCT TATTGTGACA GAGAGGGTGT ATGCTCCAGC TTCTACCTTG GTAGATCAGC 
     3961 CTTATGCTAA TGAAGGTACA GTTGTGGTCA CTGAAAGAGT AATACAGCCT CATGGGGGTG 
     4021 GATCGAATCC TCTGGAAGGC ACTCAGCATC TTCAAGATGT ACCTTACGTC ATGGTGAGGG 
     4081 AAAGAGAGAG CTTCCTTGCC CCCAGCTCAG GTGTGCAGCC TACTCTGGCC ATGCCTAATA 
     4141 TAGCAGTAGG ACAGAATGTG ACAGTGACAG AAAGAGTTCT AGCACCTGCT TCCACTCTGC 
     4201 AATCCAGTTA CCAGATTCCC ACTGAAAATT CTATGACGGC TAGGAACACC ACGGTGTCTG 
     4261 GAGCTGGAGT CCCTGGCCCT CTGCCAGATT TTGGTTTAGA GGAATCTGGT CATTCTAATT 
     4321 CTACCATAAC CACATCTTCC ACCAGAGTTA CCAAGCATAG CACTGTACAG CATTCTTACT 
     4381 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//