LOCUS       RC212105                8778 bp    DNA     circular  SYN 
DEFINITION  TBC1D4 (Myc-DDK-tagged)-Human TBC1 domain family, member 4 (TBC1D4)
ACCESSION   NM_014832
VERSION     NM_014832.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4935..5026 
                     /label="Myc-DDK"  
     misc_feature    5056..5641 
                     /label="PolyA signal"  
     misc_feature    5113..5132 
                     /label="Restriction Enzyme"  
     misc_feature    5917..6589 
                     /label="ColE1"  
     misc_feature    7019..7813 
                     /label="Kan/Neo"  
     misc_feature    7848..8150 
                     /label="SV40 ori"  
     misc_feature    8236..8713 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4917..4923 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4917 
                     /label="TBC1D4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCC AGCTGCATTC AGGATGAGCC GTTCCCGCAC CCCCTGGAGC 
     1081 CCGAGCCGGG CGTCTCAGCT CAGCCCGGCC CCGGGAAGCC AAGCGATAAG CGGTTCCGGC 
     1141 TGTGGTACGT TGGGGGGTCG TGCCTGGACC ACAGGACCAC GCTGCCTATG CTGCCCTGGC 
     1201 TCATGGCCGA GATCCGCAGG CGCAGCCAGA AGCCCGAGGC GGGCGGCTGC GGGGCGCCGG 
     1261 CGGCCCGAGA GGTGATCCTG GTGCTCAGCG CGCCCTTCCT GCGTTGCGTC CCCGCGCCGG 
     1321 GCGCTGGGGC CTCGGGGGGC ACTAGTCCGT CGGCCACGCA GCCCAACCCG GCGGTATTCA 
     1381 TCTTCGAGCA CAAGGCGCAG CATATCTCGC GCTTCATCCA CAACAGCCAC GACCTCACCT 
     1441 ACTTTGCCTA CCTGATCAAG GCGCAGCCCG ACGACCCCGA GTCGCAGATG GCCTGCCACG 
     1501 TTTTCCGCGC CACAGACCCC AGCCAGGTTC CTGATGTTAT TAGCAGCATA AGGCAATTAT 
     1561 CTAAAGCGGC CATGAAAGAG GATGCCAAAC CCAGCAAAGA TAATGAGGAC GCCTTTTACA 
     1621 ACTCTCAGAA GTTCGAAGTC CTGTACTGTG GAAAGGTGAC CGTGACCCAC AAGAAGGCCC 
     1681 CCTCAAGCCT CATCGATGAC TGCATGGAGA AGTTCAGCCT GCACGAACAG CAGCGCCTGA 
     1741 AGATCCAAGG GGAGCAGCGC GGTCCGGACC CAGGAGAGGA CCTGGCTGAC TTGGAGGTGG 
     1801 TGGTGCCCGG GTCCCCCGGA GACTGCCTGC CGGAGGAGGC TGACGGCACC GACACCCACC 
     1861 TTGGCTTACC TGCCGGGGCC AGCCAGCCTG CCCTGACCAG CTCTCGGGTC TGCTTCCCTG 
     1921 AGCGGATTTT GGAAGATTCT GGCTTTGATG AGCAGCAGGA GTTTCGGTCT CGGTGCAGCA 
     1981 GTGTCACCGG CGTGCAACGG AGAGTTCACG AGGGCAGCCA GAAATCCCAG CCGCGACGGA 
     2041 GACACGCGAG CGCACCCAGT CACGTCCAGC CCTCGGACTC GGAGAAGAAC AGGACCATGC 
     2101 TCTTCCAGGT TGGGCGATTT GAGATTAACC TTATCAGTCC AGACACTAAA TCAGTTGTGC 
     2161 TAGAAAAGAA TTTTAAAGAT ATCTCCTCTT GTTCTCAGGG TATAAAGCAT GTGGATCACT 
     2221 TTGGCTTTAT CTGCCGGGAG TCTCCAGAGC CTGGACTTAG CCAGTATATT TGTTATGTAT 
     2281 TCCAGTGTGC CAGCGAATCT CTGGTTGATG AGGTAATGCT GACTCTGAAA CAGGCCTTCA 
     2341 GTACGGCGGC TGCCCTGCAG AGTGCCAAGA CGCAGATTAA ACTGTGTGAG GCCTGCCCGA 
     2401 TGCACTCTTT GCATAAGCTC TGTGAAAGGA TTGAAGGTCT CTACCCACCA AGAGCCAAGC 
     2461 TGGTGATACA GAGGCATCTC TCATCACTGA CAGATAATGA GCAAGCTGAC ATCTTTGAAA 
     2521 GAGTTCAGAA AATGAAGCCA GTCAGTGACC AGGAAGAAAA TGAACTTGTG ATTTTACACC 
     2581 TGAGGCAGCT GTGTGAAGCC AAGCAGAAGA CACACGTGCA CATCGGGGAA GGCCCTTCTA 
     2641 CTATTTCAAA TAGTACAATC CCAGAAAATG CAACAAGCAG TGGAAGGTTC AAACTTGACA 
     2701 TTCTGAAAAA TAAAGCTAAG AGATCCTTAA CTAGCTCCCT GGAAAATATC TTCTCAAGGG 
     2761 GAGCTAACAG AATGAGAGGT CGGCTTGGAA GTGTGGACAG TTTTGAACGG TCCAACAGTC 
     2821 TTGCTTCAGA GAAGGACTAC TCACCAGGGG ATTCTCCACC AGGGACACCG CCAGCGTCCC 
     2881 CACCGTCCTC AGCTTGGCAA ACGTTTCCCG AAGAGGATTC CGACTCCCCG CAGTTTCGAA 
     2941 GACGGGCACA CACGTTCAGC CACCCACCTT CAAGCACAAA GAGAAAGCTG AATTTGCAGG 
     3001 ATGGGAGGGC TCAGGGTGTG CGTTCCCCTC TGCTGAGGCA GAGCTCCAGT GAACAGTGCA 
     3061 GCAATCTTTC GTCAGTTCGA CGCATGTACA AGGAGAGTAA TTCTTCCTCC AGTCTTCCAA 
     3121 GTCTTCACAC TTCCTTCTCT GCCCCTTCCT TCACTGCCCC CTCTTTCCTG AAAAGCTTTT 
     3181 ACCAGAATTC AGGTAGACTG TCCCCACAGT ATGAAAATGA AATCAGACAA GACACTGCTT 
     3241 CAGAATCAAG TGATGGAGAA GGGAGAAAAA GGACCTCATC TACCTGCAGC AATGAGTCCC 
     3301 TAAGTGTGGG AGGAACCTCT GTCACTCCTC GCCGGATCTC CTGGCGGCAG CGCATTTTCC 
     3361 TCAGGGTTGC TTCTCCCATG AACAAATCTC CCTCAGCAAT GCAACAGCAA GATGGATTGG 
     3421 ACAGGAACGA GCTGCTGTCA CTGTCCCCCC TCTCTCCAAC CATGGAGGAG GAACCGCTGG 
     3481 TTGTATTCCT GTCTGGGGAG GATGACCCAG AAAAGATTGA AGAAAGAAAG AAATCAAAAG 
     3541 AACTGAGGAG CTTGTGGAGA AAAGCTATAC ACCAACAAAT CTTGTTACTT CGAATGGAAA 
     3601 AAGAAAACCA GAAACTTGAA GGAGCAAGCA GAGATGAACT CCAGTCCAGA AAAGTTAAAT 
     3661 TAGACTATGA AGAAGTTGGT GCATGTCAGA AAGAGGTCTT AATAACTTGG GATAAGAAGT 
     3721 TGTTAAACTG CAGAGCTAAA ATCAGATGTG ATATGGAAGA TATTCATACT CTTCTTAAAG 
     3781 AAGGAGTTCC CAAAAGTCGA CGAGGAGAAA TTTGGCAGTT TCTGGCTTTA CAGTACCGAC 
     3841 TCAGACACAG ATTGCCTAAT AAACAACAGC CTCCTGACAT ATCCTATAAG GAACTTTTGA 
     3901 AGCAGCTCAC TGCTCAGCAG CATGCGATTC TCGTGGATTT AGGAAGGACG TTTCCTACTC 
     3961 ACCCTTACTT TTCAGTACAG CTTGGGCCAG GACAGCTGTC ACTGTTTAAC CTCCTGAAAG 
     4021 CCTATTCTTT GCTGGACAAA GAAGTGGGAT ACTGTCAGGG GATCAGCTTT GTGGCTGGAG 
     4081 TCCTGCTTCT GCACATGAGT GAAGAGCAAG CCTTTGAAAT GCTGAAATTC CTCATGTATG 
     4141 ACCTCGGCTT CCGCAAGCAG TACAGACCTG ACATGATGTC GCTGCAGATT CAAATGTACC 
     4201 AGCTGTCCAG GCTCCTTCAT GACTATCACA GAGATCTCTA CAATCACCTT GAAGAAAATG 
     4261 AAATCAGCCC CAGTCTTTAT GCTGCCCCCT GGTTCCTCAC ATTGTTTGCC TCTCAGTTTT 
     4321 CATTAGGATT TGTAGCCAGA GTTTTTGATA TTATTTTTCT TCAGGGAACT GAAGTTATAT 
     4381 TCAAGGTTGC ACTCAGCCTA CTGAGCAGCC AAGAGACACT TATAATGGAA TGTGAGAGCT 
     4441 TTGAAAATAT TGTTGAGTTT CTTAAAAACA CGCTACCTGA TATGAATACC TCTGAAATGG 
     4501 AAAAAATTAT TACCCAGGTT TTTGAGATGG ATATTTCTAA GCAGTTGCAT GCCTATGAGG 
     4561 TGGAATATCA TGTGCTACAG GATGAGCTTC AGGAATCTTC ATATTCCTGT GAGGATAGTG 
     4621 AAACTTTGGA GAAGCTGGAG AGGGCCAATA GCCAACTGAA AAGACAAAAC ATGGACCTCC 
     4681 TAGAAAAATT ACAGGTAGCT CATACTAAAA TCCAGGCCTT GGAATCAAAC CTGGAAAATC 
     4741 TTTTGACGAG AGAGACCAAA ATGAAGTCTT TAATCCGGAC CCTGGAACAA GAAAAAATGG 
     4801 CTTATCAAAA GACAGTGGAG CAACTCCGGA AGCTGCTGCC CGCGGATGCT CTAGTCAATT 
     4861 GTGACCTGTT GCTGAGAGAC CTAAACTGCA ACCCTAACAA CAAAGCCAAG ATAGGAAATA 
     4921 AGCCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4981 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5041 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5101 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5161 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5221 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5281 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5341 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5401 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5461 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5521 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5581 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5641 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5701 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5761 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5821 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5881 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5941 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6001 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6061 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6121 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6181 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6241 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6301 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6361 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6421 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6481 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6541 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6601 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6661 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6721 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6781 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6841 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6901 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6961 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7021 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7081 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7141 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7201 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7261 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7321 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7381 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7441 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7501 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7561 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7621 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7681 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7741 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7801 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7861 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7921 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7981 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8221 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8281 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8341 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8401 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8461 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8521 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8581 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8641 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8701 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8761 AAATTTAACG CGAATTTT
//