LOCUS       RC212075                7914 bp    DNA     circular  SYN 
DEFINITION  RASAL3 (Myc-DDK-tagged)-Human RAS protein activator like 3 (RASAL3)
ACCESSION   NM_022904
VERSION     NM_022904.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4071..4162 
                     /label="Myc-DDK"  
     misc_feature    4192..4777 
                     /label="PolyA signal"  
     misc_feature    4249..4268 
                     /label="Restriction Enzyme"  
     misc_feature    5053..5725 
                     /label="ColE1"  
     misc_feature    6155..6949 
                     /label="Kan/Neo"  
     misc_feature    6984..7286 
                     /label="SV40 ori"  
     misc_feature    7372..7849 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4053..4059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4053 
                     /label="RASAL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCCACCG TCGCCAAGCC GGACCTCCCA AACCCAGCCC ACAGCCACCT 
     1081 CTCCGCTGAC TTCCTACCGC TGGCACACAG GGGGCGGTGG GGAGAAGGCG GCTGGAGGGT 
     1141 TCCGCTGGGG CCGCTTTGCT GGCTGGGGCA GGGCCCTGAG CCACCAGGAG CCCATGGTCA 
     1201 GCACCCAGCC AGCCCCTCGC TCGATATTCC GTCGGGTCCT ATCTGCGCCT CCCAAGGAGT 
     1261 CACGGACCAG TCGCCTTCGA CTCTCCAAGG CCCTCTGGGG GAGGCATAAG AACCCACCGC 
     1321 CGGAGCCAGA CCCGGAGCCG GAGCAGGAGG CCCCAGAGCT GGAGCCGGAG CCAGAGCTGG 
     1381 AGCCCCCTAC CCCACAGATC CCTGAGGCCC CCACACCCAA CGTGCCTGTC TGGGACATTG 
     1441 GGGGCTTCAC CCTGCTTGAT GGGAAGCTGG TGCTGCTTGG AGGAGAGGAG GAGGGTCCTC 
     1501 GAAGGCCCCG GGTGGGAAGT GCTAGCTCCG AGGGCAGCAT CCACGTGGCC ATGGGGAACT 
     1561 TCAGGGATCC AGATCGGATG CCTGGAAAAA CAGAACCGGA GACTGCTGGT CCCAACCAGG 
     1621 TCCACAACGT TCGGGGGTTG CTCAAGAGGC TGAAAGAGAA GAAAAAGGCC AGGTTGGAGC 
     1681 CCCGGGATGG ACCCCCCAGT GCTCTGGGCT CTAGGGAGTC GCTGGCCACA CTCTCTGAAC 
     1741 TGGACCTGGG TGCCGAGCGG GATGTGCGGA TCTGGCCACT GCACCCCAGC CTCCTGGGGG 
     1801 AGCCCCACTG CTTTCAGGTA ACCTGGACGG GTGGAAGCCG CTGCTTCTCT TGTCGCTCGG 
     1861 CCGCTGAGAG AGACCGCTGG ATCGAGGACC TTCGTCGCCA ATTCCAGCCC ACCCAGGACA 
     1921 ACGTGGAGCG GGAAGAGACA TGGCTGAGCG TGTGGGTGCA CGAAGCGAAG GGGCTTCCCC 
     1981 GAGCAGCGGC GGGGGCACCC GGCGTGCGCG CCGAGCTGTG GCTGGATGGC GCGCTGCTGG 
     2041 CACGCACGGC GCCTCGGGCC GGCCCAGGCC AGCTCTTCTG GGCCGAGCGC TTCCACTTCG 
     2101 AGGCGCTGCC ACCGGCACGT CGCCTGTCGC TGCGGCTGCG CGGCTTGGGC CCGGGAAGCG 
     2161 CGGTGCTGGG CCGCGTGGCC CTGGCGCTGG AGGAGCTGGA CGCCCCACGC GCGCCTGCCG 
     2221 CCGGTCTGGA GCGCTGGTTC CCGCTGCTCG GGGCGCCGGC GGGCGCAGCG CTGCGGGCGC 
     2281 GGATTCGGGC GCGTCGCCTG CGCGTGCTGC CGTCCGAGCG CTACAAGGAG CTGGCGGAGT 
     2341 TCCTCACCTT CCACTATGCG CGCCTCTGCG GGGCCCTGGA GCCCGCGCTG CCTGCGCAGG 
     2401 CCAAGGAGGA GCTGGCGGCA GCCATGGTGC GCGTGCTGCG GGCCACCGGC CGGGCGCAGG 
     2461 CGCTGGTGAC TGACCTGGGC ACTGCGGAGC TGGCGCGCTG TGGAGGCCGT GAGGCGCTGC 
     2521 TGTTCCGGGA AAACACATTG GCCACCAAGG CTATCGATGA GTACATGAAG CTCGTGGCAC 
     2581 AGGATTACCT CCAGGAGACC CTGGGACAGG TTGTGCGGCG TCTCTGTGCT TCTACTGAGG 
     2641 ACTGTGAAGT GGACCCCAGC AAATGTCCAG CCTCGGAGCT GCCAGAGCAC CAGGCCAGAC 
     2701 TTCGGAACAG CTGCGAGGAG GTCTTCGAAA CCATTATCCA TTCCTACGAC TGGTTCCCTG 
     2761 CGGAGCTGGG CATCGTGTTC TCAAGCTGGC GAGAAGCATG TAAAGAACGT GGCTCTGAGG 
     2821 TGCTGGGCCC CCGACTGGTG TGCGCCTCCC TCTTCCTGCG GCTCCTGTGC CCTGCCATCC 
     2881 TGGCACCCAG CCTCTTTGGT TTGGCACCAG ACCATCCAGC ACCCGGCCCA GCCCGCACCC 
     2941 TCACACTGAT TGCCAAGGTC ATCCAGAACC TCGCCAACCG TGCCCCGTTC GGTGAGAAGG 
     3001 AGGCCTACAT GGGCTTCATG AATAGCTTCC TGGAGGAACA TGGACCAGCC ATGCAATGCT 
     3061 TCCTGGACCA GGTAGCCATG GTGGATGTGG ATGCTGCCCC CAGTGGTTAC CAGGGCAGTG 
     3121 GTGATCTGGC CCTCCAGTTA GCTGTCCTGC ATGCCCAGCT CTGTACAATT TTTGCTGAGC 
     3181 TTGACCAGAC AACCCGAGAC ACCCTGGAAC CACTGCCCAC CATCCTGCGA GCCATTGAGG 
     3241 AGGGCCAGCC TGTGCTTGTG TCAGTGCCAA TGCGTCTCCC ACTGCCCCCG GCCCAGGTCC 
     3301 ACTCCAGCCT CTCCGCAGGG GAGAAGCCCG GCTTCCTGGC CCCCCGGGAC CTCCCCAAGC 
     3361 ACACCCCTCT CATCTCCAAG AGCCAGTCTC TGCGCAGCGT TCGCCGCTCA GAGAGTTGGG 
     3421 CCCGGCCACG GCCGGACGAA GAGCGGCCCC TGCGGCGGCC CCGGCCGGTG CAGCGCACGC 
     3481 AGAGTGTCCC GGTCCGGCGT CCTGCCCGCC GCCGCCAATC TGCGGGGCCC TGGCCGCGAC 
     3541 CCAAAGGCTC CCTGAGCATG GGACCAGCGC CCCGCGCCCG GCCTTGGACC CGGGACTCCG 
     3601 CCTCGCTGCC TCGGAAGCCG TCGGTACCCT GGCAGCGCCA AATGGACCAG CCGCAAGACC 
     3661 GAAACCAGGC ACTGGGCACG CACCGACCTG TGAACAAGTT GGCAGAGCTG CAGTGCGAGG 
     3721 TGGCCGCTCT GCGTGAGGAG CAGAAAGTGC TGTCCCGCCT CGTGGAGTCG CTGAGCACCC 
     3781 AAATCCGGGC CTTGACGGAG CAGCAGGAGC AGCTGCGGGG CCAGCTGCAG GATCTGGACT 
     3841 CCAGGCTCCG TGCTGGGAGC TCAGAGTTTG ATTCAGAGCA CAACCTAACA AGCAATGAAG 
     3901 GGCACAGTCT GAAAAACCTG GAGCACCGCC TAAATGAGAT GGAGAGAACT CAGGCTCAGC 
     3961 TGAGGGATGC TGTCCAGAGC CTGCAGCTTT CTCCAAGGAC GCGGGGGTCT TGGAGTCAAC 
     4021 CCCAGCCCCT CAAAGCACCC TGCCTCAATG GAGACACCAC CACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//