LOCUS       RC211975                8715 bp    DNA     circular  SYN 
DEFINITION  DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 1
ACCESSION   NM_004082
VERSION     NM_004082.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4872..4963 
                     /label="Myc-DDK"  
     misc_feature    4993..5578 
                     /label="PolyA signal"  
     misc_feature    5050..5069 
                     /label="Restriction Enzyme"  
     misc_feature    5854..6526 
                     /label="ColE1"  
     misc_feature    6956..7750 
                     /label="Kan/Neo"  
     misc_feature    7785..8087 
                     /label="SV40 ori"  
     misc_feature    8173..8650 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4854..4860 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4854 
                     /label="DCTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAGAGC AAGAGGCACG TGTACAGCCG GACGCCCAGC GGCAGCAGGA 
     1081 TGAGTGCGGA GGCAAGCGCC CGGCCTCTGC GGGTGGGCTC CCGTGTAGAG GTGATTGGAA 
     1141 AAGGCCACCG AGGCACTGTG GCCTATGTTG GAGCCACACT GTTTGCCACT GGCAAATGGG 
     1201 TAGGCGTGAT TCTGGATGAA GCAAAGGGCA AAAATGATGG AACTGTTCAA GGCAGGAAGT 
     1261 ACTTCACTTG TGATGAAGGG CATGGCATCT TTGTGCGCCA GTCCCAGATC CAGGTATTTG 
     1321 AAGATGGAGC AGATACTACT TCCCCAGAGA CACCTGATTC TTCTGCTTCA AAAGTCCTCA 
     1381 AAAGAGAGGG AACTGATACA ACTGCAAAGA CTAGCAAACT GCGGGGACTG AAGCCTAAGA 
     1441 AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC CCAGCCAGTA 
     1501 CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA GCAGGTGAGC 
     1561 TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC ATCATCCCCA 
     1621 CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC AAGGAGGAGG 
     1681 AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG AGACTGAAAC 
     1741 GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG CTGGAGCAGG 
     1801 TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG CGCCTCAAGG 
     1861 AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG GAGGAGATGG 
     1921 CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT GAAGAGCGGG 
     1981 CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG CTCACTACTG 
     2041 ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT GCATCCAGTT 
     2101 ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG GTGAGGATGC 
     2161 GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC ATGGAAAAGA 
     2221 AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG GAGCTAAGCC 
     2281 AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG GGTGCTGAGG 
     2341 AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG CGCGAGTTGA 
     2401 GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG CAGGAGAATG 
     2461 CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG CGGGTTCGTG 
     2521 AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG CAGACCATCA 
     2581 AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG ACAAACCAGC 
     2641 AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC TTCAAAATCA 
     2701 AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG CAGATGGAGG 
     2761 TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC AGCTTCCTTC 
     2821 GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT CTCATTTGCA 
     2881 AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG AACTGTTCAG 
     2941 AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT GGACTGGTGT 
     3001 ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC TCTCAGTGCA 
     3061 GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC CATGAGCGCT 
     3121 CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT GTCAATGTGG 
     3181 AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC CTTGCCGAAC 
     3241 AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG AGTGCTCTGG 
     3301 ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG CAGGAGGCTA 
     3361 CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC CGCCAGTTCT 
     3421 GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA GCTGCACTGG 
     3481 CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG ACGTGGGTCG 
     3541 TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA CTGGCAGAGA 
     3601 ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC GAGCAGATCT 
     3661 ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC ATCCTCATCA 
     3721 GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA GAGCGGCCCC 
     3781 CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG ATCACAGATG 
     3841 CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG TTGAAGAAGT 
     3901 CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC CTCCTGGAGA 
     3961 AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC CAGACTCGGC 
     4021 TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG ACAATGGATG 
     4081 CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG CAGCGTCTGA 
     4141 ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA GGCATTGCTA 
     4201 CTCTGGTCTC TGGCATTGCT GGTGAAGAAC AGCAGCGAGG AGCCATCCCT GGGCAGGCTC 
     4261 CAGGGTCTGT GCCAGGCCCA GGGCTGGTGA AGGACTCACC ACTGCTGCTT CAGCAGATCT 
     4321 CTGCCATGAG GCTGCACATC TCCCAGCTCC AGCATGAGAA CAGCATCCTC AAGGGAGCCC 
     4381 AGATGAAGGC ATCCTTGGCA TCCCTGCCCC CTCTGCATGT TGCAAAGCTA TCCCATGAGG 
     4441 GCCCTGGCAG TGAGTTACCA GCTGGAGCGC TGTATCGTAA GACCAGCCAG CTGCTGGAGA 
     4501 CATTGAATCA ATTGAGCACA CACACGCACG TAGTAGACAT CACTCGCACC AGCCCTGCTG 
     4561 CCAAGAGCCC GTCGGCCCAA CTTATGGAGC AAGTGGCTCA GCTTAAGTCC CTGAGTGACA 
     4621 CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC CCTGGAGCCA 
     4681 CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC AAGGAGGAGC 
     4741 AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT GGTTTTGGAC 
     4801 AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACAGT CGCCTCATCT 
     4861 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4921 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4981 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5041 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5101 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5161 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5221 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5281 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5341 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5401 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5461 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5521 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5581 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5641 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5701 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5761 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5821 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5881 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5941 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6001 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6061 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6121 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6181 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6241 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6301 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6361 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6421 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6481 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6541 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6601 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6661 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6721 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6781 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6841 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6901 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6961 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7021 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7081 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7141 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7201 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7261 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7321 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7381 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7441 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7501 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7561 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7621 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7681 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7741 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7801 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7861 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7921 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8161 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8221 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8281 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8341 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8401 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8461 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8521 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8581 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8641 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8701 TTTAACGCGA ATTTT
//