LOCUS       RC211969                6678 bp    DNA     circular  SYN 
DEFINITION  AFM (Myc-DDK-tagged)-Human afamin (AFM)
ACCESSION   NM_001133
VERSION     NM_001133.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2835..2926 
                     /label="Myc-DDK"  
     misc_feature    2956..3541 
                     /label="PolyA signal"  
     misc_feature    3013..3032 
                     /label="Restriction Enzyme"  
     misc_feature    3817..4489 
                     /label="ColE1"  
     misc_feature    4919..5713 
                     /label="Kan/Neo"  
     misc_feature    5748..6050 
                     /label="SV40 ori"  
     misc_feature    6136..6613 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2817..2823 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2817 
                     /label="AFM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACTACTA AAACTTACAG GTTTTATTTT TTTCTTGTTT TTTTTGACTG 
     1081 AATCCCTAAC CCTGCCCACA CAACCTCGGG ATATAGAGAA CTTCAATAGT ACTCAAAAAT 
     1141 TTATAGAAGA TAATATTGAA TACATCACCA TCATTGCATT TGCTCAGTAT GTTCAGGAAG 
     1201 CAACCTTTGA AGAAATGGAA AAGCTGGTGA AAGACATGGT AGAATACAAA GACAGATGTA 
     1261 TGGCTGACAA GACGCTCCCA GAGTGTTCAA AATTACCTAA TAATGTTTTA CAGGAAAAAA 
     1321 TATGTGCTAT GGAGGGGCTG CCACAAAAGC ATAATTTCTC ACACTGCTGC AGTAAGGTTG 
     1381 ATGCTCAAAG AAGACTCTGT TTCTTCTATA ACAAGAAATC TGATGTGGGA TTTCTGCCTC 
     1441 CTTTCCCTAC CCTGGATCCC GAAGAGAAAT GCCAGGCTTA TGAAAGTAAC AGAGAATCCC 
     1501 TTTTAAATCA CTTTTTATAT GAAGTTGCCA GAAGGAACCC ATTTGTCTTC GCCCCTACAC 
     1561 TTCTAACTGT TGCTGTTCAT TTTGAGGAGG TGGCCAAATC ATGTTGTGAA GAACAAAACA 
     1621 AAGTCAACTG CCTTCAAACA AGGGCAATAC CTGTCACACA ATATTTAAAA GCATTTTCTT 
     1681 CTTATCAAAA ACATGTCTGT GGGGCACTTT TGAAATTTGG AACCAAAGTT GTACACTTTA 
     1741 TATATATTGC GATACTCAGT CAAAAATTCC CCAAGATTGA ATTTAAGGAG CTTATTTCTC 
     1801 TTGTAGAAGA TGTTTCTTCC AACTATGATG GATGCTGTGA AGGGGATGTT GTGCAGTGCA 
     1861 TCCGTGACAC GAGCAAGGTT ATGAACCATA TTTGTTCAAA ACAAGATTCT ATCTCCAGCA 
     1921 AAATCAAAGA GTGCTGTGAA AAGAAAATAC CAGAGCGCGG CCAGTGCATA ATTAACTCAA 
     1981 ACAAAGATGA TAGACCAAAG GATTTATCTC TAAGAGAAGG AAAATTTACT GACAGTGAAA 
     2041 ATGTGTGTCA AGAACGAGAT GCTGACCCAG ACACCTTCTT TGCGAAGTTT ACTTTTGAAT 
     2101 ACTCAAGGAG ACATCCAGAC CTGTCTATAC CAGAGCTTTT AAGAATTGTT CAAATATACA 
     2161 AAGATCTCCT GAGAAATTGC TGCAACACAG AAAACCCTCC AGGTTGTTAC CGTTACGCGG 
     2221 AAGACAAATT CAATGAGACA ACTGAGAAAA GCCTCAAGAT GGTACAACAA GAATGTAAAC 
     2281 ATTTCCAGAA TTTGGGGAAG GATGGTTTGA AATACCATTA CCTCATCAGG CTCACGAAGA 
     2341 TAGCTCCCCA ACTCTCCACT GAAGAACTGG TGTCTCTTGG CGAGAAAATG GTGACAGCTT 
     2401 TCACTACTTG CTGTACGCTA AGTGAAGAGT TTGCCTGTGT TGATAATTTG GCAGATTTAG 
     2461 TTTTTGGAGA GTTATGTGGA GTAAATGAAA ATCGAACTAT CAACCCTGCT GTGGACCACT 
     2521 GCTGTAAAAC AAACTTTGCC TTCAGAAGGC CCTGCTTTGA GAGTTTGAAA GCTGATAAAA 
     2581 CATATGTGCC TCCACCTTTC TCTCAAGATT TATTTACCTT TCACGCAGAC ATGTGTCAAT 
     2641 CTCAGAATGA GGAGCTTCAG AGGAAGACAG ACAGGTTTCT TGTCAACTTA GTGAAGCTGA 
     2701 AGCATGAACT CACAGATGAA GAGCTGCAGT CTTTGTTTAC AAATTTCGCA AATGTAGTGG 
     2761 ATAAGTGCTG CAAAGCAGAG AGTCCTGAAG TCTGCTTTAA TGAAGAGAGT CCAAAAATTG 
     2821 GCAACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     2881 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     2941 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3001 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3061 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3121 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3181 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3241 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3301 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3361 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3421 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3481 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3541 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3601 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3661 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3721 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3781 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3841 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     3901 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     3961 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4021 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4081 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4141 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4201 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4261 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4321 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4381 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4441 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4501 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4561 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4621 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4681 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4741 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4801 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4861 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     4921 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     4981 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5041 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5101 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5161 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5221 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5281 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5341 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5401 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5461 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5521 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5581 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5641 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5701 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5761 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5821 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     5881 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     5941 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6001 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6061 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6121 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6181 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6241 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6301 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6361 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6421 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6481 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6541 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6601 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6661 AAATTTAACG CGAATTTT
//