LOCUS       RC211950                8757 bp    DNA     circular  SYN 
DEFINITION  UBE2O (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2O (UBE2O)
ACCESSION   NM_022066
VERSION     NM_022066.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4914..5005 
                     /label="Myc-DDK"  
     misc_feature    5035..5620 
                     /label="PolyA signal"  
     misc_feature    5092..5111 
                     /label="Restriction Enzyme"  
     misc_feature    5896..6568 
                     /label="ColE1"  
     misc_feature    6998..7792 
                     /label="Kan/Neo"  
     misc_feature    7827..8129 
                     /label="SV40 ori"  
     misc_feature    8215..8692 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4896..4902 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4896 
                     /label="UBE2O"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATCCC GCAGCCCCCA CGCCCGCAGC TCCCGCTCCA GCCCAGGCCC 
     1081 CGGCTCCAGC CCCGGAGGCA GTCCCGGCCC CAGCCGCAGC CCCCGTCCCG GCGCCGGCGC 
     1141 CCGCCTCGGA CTCGGCCTCC GGGCCGTCCT CGGACTCCGG CCCAGAAGCC GGCTCGCAGC 
     1201 GCCTGCTGTT TTCTCACGAC CTGGTGTCGG GCCGTTACCG TGGCTCCGTG CACTTCGGGC 
     1261 TGGTGCGCCT CATCCACGGC GAGGACTCGG ACTCGGAGGG CGAGGAGGAG GGCCGCGGGA 
     1321 GCTCGGGGTG CTCCGAGGCC GGGGGCGCGG GCCACGAGGA GGGCCGGGCC AGCCCCCTGC 
     1381 GCCGCGGCTA CGTGCGCGTC CAGTGGTACC CGGAGGGCGT CAAGCAGCAT GTGAAGGAGA 
     1441 CCAAGCTGAA ACTAGAGGAC CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT 
     1501 CCACCGACAG TCAGTGTGGC ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA 
     1561 TCGGCACCAA CTGCATCATC TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT 
     1621 TCATGTATGG GGACTACATT GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA 
     1681 ACCAGATCAT CCTGAAGCTA TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG 
     1741 CCAAGCTCTA CGACGTCTGC CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT 
     1801 ATGGCTTCTA CCCAGGCCAG GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT 
     1861 GGCTGTCAGG TGTCAAGCCC GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG 
     1921 AGGTGCAGGT TGTAGAGTTG AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA 
     1981 CGGACAGCGT CAGCCCCCCA CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC 
     2041 GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC 
     2101 CAGCCAAGGT AGAGCCAGCC AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG 
     2161 GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA 
     2221 TGTCATGCTC CCCAGACACC CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA 
     2281 AGGGGGAATC CAAAACCAAG AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG 
     2341 GCTCTGCCAG TCCAGTGGAG ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG 
     2401 AGCAGCTGCC CCCATTCCTG CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC 
     2461 AGGACGCAGA TGATGAGGCT GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG 
     2521 CCAGCTCCAC CACTTCCTCC CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT 
     2581 TGTCCATCAA GAACTTAAAG CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT 
     2641 TCAAGCCAGG GGACAGGGTG GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA 
     2701 TGTGGCAGGA TGGCTCCGTG GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC 
     2761 ACCTGGACAA CAACGAGTTC TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT 
     2821 GTCCAGACCC TGCTGTCTAC GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA 
     2881 TGGTGAAGTG GTTCAAGCTG AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG 
     2941 AAGATGTGAG TGTTTACGAC ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA 
     3001 TCGTCATCCG CATCGGCAAT ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG 
     3061 TGGGCCAGGT GGCCCGTGTG GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT 
     3121 CAAAGACCAT CATCCTGCCC CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG 
     3181 ACTACGATTC GGTAGAAGGC AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA 
     3241 GTGACAGCTG GGAGACGGAC AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC 
     3301 CCCCCATCCC ACCCCTGGAG CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG 
     3361 AAGAGGCAGC CACAGCTGCC GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC 
     3421 TGATGGAGAA GGCTGGCAAG GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA 
     3481 TCAAGATCCT GGAGAGCCTC AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA 
     3541 CCTCTCCGAC TGTGGAGCCT GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA 
     3601 AGAAGCTACA GGAAAACCTC AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA 
     3661 AGATGGAAGC AGTGCCCGAC GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG 
     3721 TGAAGGCTGA GTGGCCCAGC GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG 
     3781 GCGTCACCTT CACCAGCGCC AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA 
     3841 ATCATTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG 
     3901 TGCGGAAGGA GATGGCGCTG CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT 
     3961 TTGAAGATAG AATGGACCTC TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG 
     4021 AGGATGGCCT CTACTTGTTT GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC 
     4081 ACTTCTGCTA CCTCTCCCAA TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA 
     4141 AGGTGTGTGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA 
     4201 AGTCCAGCCT TCTCCAGGTG CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT 
     4261 ACTACAACGA AGCCGGCTTC GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC 
     4321 GCTGTTACAA TGAGATGGCG CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC 
     4381 GGCCCCCCGA GGTCTTTGAG CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC 
     4441 TGGTGAACCG TATCGAGTCC TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC 
     4501 TGCCCAACGG GGTGCCCAAG GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT 
     4561 CAGACTCCGG CCAACAAGAA CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG 
     4621 GCTCAGACTC AGAGGGCGGT GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC 
     4681 AGACTTCGGA GACCGCACCA GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA 
     4741 GAAAGAGCTA CCGGAGCTTC TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC 
     4801 TCTTCCCACT TTCCAAGGGT TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG 
     4861 CTGCCCTGCT AGAGGCAGGC ATGCCGGAGT GCACAGAGGA CAAGACGCGT ACGCGGCCGC 
     4921 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4981 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5041 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5101 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5161 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5221 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5281 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5341 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5401 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5461 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5521 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5581 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5641 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5701 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5761 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5821 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5881 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5941 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6001 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6061 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6121 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6181 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6241 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6301 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6361 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6421 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6481 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6541 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6601 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6661 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6721 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6781 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6841 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6901 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6961 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7021 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7081 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7141 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7201 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7261 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7321 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7381 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7441 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7501 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7561 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7621 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7681 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7741 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7801 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7861 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7921 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7981 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8161 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8221 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8281 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8341 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8401 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8461 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8521 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8581 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8641 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8701 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//