LOCUS       RC211925                8595 bp    DNA     circular  SYN 
DEFINITION  JAG2 (Myc-DDK-tagged)-Human jagged 2 (JAG2), transcript variant 1
ACCESSION   NM_002226
VERSION     NM_002226.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4752..4843 
                     /label="Myc-DDK"  
     misc_feature    4873..5458 
                     /label="PolyA signal"  
     misc_feature    4930..4949 
                     /label="Restriction Enzyme"  
     misc_feature    5734..6406 
                     /label="ColE1"  
     misc_feature    6836..7630 
                     /label="Kan/Neo"  
     misc_feature    7665..7967 
                     /label="SV40 ori"  
     misc_feature    8053..8530 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4734..4740 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4734 
                     /label="JAG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGCAG GGCCGGGGGC GCCTTCCCCG GCGGCTGCTG CTGCTGCTGG 
     1081 CGCTCTGGGT GCAGGCGGCG CGGCCCATGG GCTATTTCGA GCTGCAGCTG AGCGCGCTGC 
     1141 GGAACGTGAA CGGGGAGCTG CTGAGCGGCG CCTGCTGTGA CGGCGACGGC CGGACAACGC 
     1201 GCGCGGGGGG CTGCGGCCAC GACGAGTGCG ACACGTACGT GCGCGTGTGC CTTAAGGAGT 
     1261 ACCAGGCCAA GGTGACGCCC ACGGGGCCCT GCAGCTACGG CCACGGCGCC ACGCCCGTGC 
     1321 TGGGCGGCAA CTCCTTCTAC CTGCCGCCGG CGGGCGCTGC GGGGGACCGA GCGCGGGCGC 
     1381 GGGCCCGGGC CGGCGGCGAC CAGGACCCGG GCCTCGTCGT CATCCCCTTC CAGTTCGCCT 
     1441 GGCCGCGCTC CTTTACCCTC ATCGTGGAGG CCTGGGACTG GGACAACGAT ACCACCCCGA 
     1501 ATGAGGAGCT GCTGATCGAG CGAGTGTCGC ATGCCGGCAT GATCAACCCG GAGGACCGCT 
     1561 GGAAGAGCCT GCACTTCAGC GGCCACGTGG CGCACCTGGA GCTGCAGATC CGCGTGCGCT 
     1621 GCGACGAGAA CTACTACAGC GCCACTTGCA ACAAGTTCTG CCGGCCCCGC AACGACTTTT 
     1681 TCGGCCACTA CACCTGCGAC CAGTACGGCA ACAAGGCCTG CATGGACGGC TGGATGGGCA 
     1741 AGGAGTGCAA GGAAGCTGTG TGTAAACAAG GGTGTAATTT GCTCCACGGG GGATGCACCG 
     1801 TGCCTGGGGA GTGCAGGTGC AGCTACGGCT GGCAAGGGAG GTTCTGCGAT GAGTGTGTCC 
     1861 CCTACCCCGG CTGCGTGCAT GGCAGTTGTG TGGAGCCCTG GCAGTGCAAC TGTGAGACCA 
     1921 ACTGGGGCGG CCTGCTCTGT GACAAAGACC TGAACTACTG TGGCAGCCAC CACCCCTGCA 
     1981 CCAACGGAGG CACGTGCATC AACGCCGAGC CTGACCAGTA CCGCTGCACC TGCCCTGACG 
     2041 GCTACTCGGG CAGGAACTGT GAGAAGGCTG AGCACGCCTG CACCTCCAAC CCGTGTGCCA 
     2101 ACGGGGGCTC TTGCCATGAG GTGCCGTCCG GCTTCGAATG CCACTGCCCA TCGGGCTGGA 
     2161 GCGGGCCCAC CTGTGCCCTT GACATCGATG AGTGTGCTTC GAACCCGTGT GCGGCCGGTG 
     2221 GCACCTGTGT GGACCAGGTG GACGGCTTTG AGTGCATCTG CCCCGAGCAG TGGGTGGGGG 
     2281 CCACCTGCCA GCTGGACGCC AATGAGTGTG AAGGGAAGCC ATGCCTTAAC GCTTTTTCTT 
     2341 GCAAAAACCT GATTGGCGGC TATTACTGTG ATTGCATCCC GGGCTGGAAG GGCATCAACT 
     2401 GCCATATCAA CGTCAACGAC TGTCGCGGGC AGTGTCAGCA TGGGGGCACC TGCAAGGACC 
     2461 TGGTGAACGG GTACCAGTGT GTGTGCCCAC GGGGCTTCGG AGGCCGGCAT TGCGAGCTGG 
     2521 AACGAGACAA GTGTGCCAGC AGCCCCTGCC ACAGCGGCGG CCTCTGCGAG GACCTGGCCG 
     2581 ACGGCTTCCA CTGCCACTGC CCCCAGGGCT TCTCCGGGCC TCTCTGTGAG GTGGATGTCG 
     2641 ACCTTTGTGA GCCAAGCCCC TGCCGGAACG GCGCTCGCTG CTATAACCTG GAGGGTGACT 
     2701 ATTACTGCGC CTGCCCTGAT GACTTTGGTG GCAAGAACTG CTCCGTGCCC CGCGAGCCGT 
     2761 GCCCTGGCGG GGCCTGCAGA GTGATCGATG GCTGCGGGTC AGACGCGGGG CCTGGGATGC 
     2821 CTGGCACAGC AGCCTCCGGC GTGTGTGGCC CCCATGGACG CTGCGTCAGC CAGCCAGGGG 
     2881 GCAACTTTTC CTGCATCTGT GACAGTGGCT TTACTGGCAC CTACTGCCAT GAGAACATTG 
     2941 ACGACTGCCT GGGCCAGCCC TGCCGCAATG GGGGCACATG CATCGATGAG GTGGACGCCT 
     3001 TCCGCTGCTT CTGCCCCAGC GGCTGGGAGG GCGAGCTCTG CGACACCAAT CCCAACGACT 
     3061 GCCTTCCCGA TCCCTGCCAC AGCCGCGGCC GCTGCTACGA CCTGGTCAAT GACTTCTACT 
     3121 GTGCGTGCGA CGACGGCTGG AAGGGCAAGA CCTGCCACTC ACGCGAGTTC CAGTGCGATG 
     3181 CCTACACCTG CAGCAACGGT GGCACCTGCT ACGACAGCGG CGACACCTTC CGCTGCGCCT 
     3241 GCCCCCCCGG CTGGAAGGGC AGCACCTGCG CCGTCGCCAA GAACAGCAGC TGCCTGCCCA 
     3301 ACCCCTGTGT GAATGGTGGC ACCTGCGTGG GCAGCGGGGC CTCCTTCTCC TGCATCTGCC 
     3361 GGGACGGCTG GGAGGGTCGT ACTTGCACTC ACAATACCAA CGACTGCAAC CCTCTGCCTT 
     3421 GCTACAATGG TGGCATCTGT GTTGACGGCG TCAACTGGTT CCGCTGCGAG TGTGCACCTG 
     3481 GCTTCGCGGG GCCTGACTGC CGCATCAACA TCGACGAGTG CCAGTCCTCG CCCTGTGCCT 
     3541 ACGGGGCCAC GTGTGTGGAT GAGATCAACG GGTATCGCTG TAGCTGCCCA CCCGGCCGAG 
     3601 CCGGCCCCCG GTGCCAGGAA GTGATCGGGT TCGGGAGATC CTGCTGGTCC CGGGGCACTC 
     3661 CGTTCCCACA CGGAAGCTCC TGGGTGGAAG ACTGCAACAG CTGCCGCTGC CTGGATGGCC 
     3721 GCCGTGACTG CAGCAAGGTG TGGTGCGGAT GGAAGCCTTG TCTGCTGGCC GGCCAGCCCG 
     3781 AGGCCCTGAG CGCCCAGTGC CCACTGGGGC AAAGGTGCCT GGAGAAGGCC CCAGGCCAGT 
     3841 GTCTGCGACC ACCCTGTGAG GCCTGGGGGG AGTGCGGCGC AGAAGAGCCA CCGAGCACCC 
     3901 CCTGCCTGCC ACGCTCCGGC CACCTGGACA ATAACTGTGC CCGCCTCACC TTGCATTTCA 
     3961 ACCGTGACCA CGTGCCCCAG GGCACCACGG TGGGCGCCAT TTGCTCCGGG ATCCGCTCCC 
     4021 TGCCAGCCAC AAGGGCTGTG GCACGGGACC GCCTGCTGGT GTTGCTTTGC GACCGGGCGT 
     4081 CCTCGGGGGC CAGTGCCGTG GAGGTGGCCG TGTCCTTCAG CCCTGCCAGG GACCTGCCTG 
     4141 ACAGCAGCCT GATCCAGGGC GCGGCCCACG CCATCGTGGC CGCCATCACC CAGCGGGGGA 
     4201 ACAGCTCACT GCTCCTGGCT GTCACCGAGG TCAAGGTGGA GACGGTTGTT ACGGGCGGCT 
     4261 CTTCCACAGG TCTGCTGGTG CCTGTGCTGT GTGGTGCCTT CAGCGTGCTG TGGCTGGCGT 
     4321 GCGTGGTCCT GTGCGTGTGG TGGACACGCA AGCGCAGGAA AGAGCGGGAG AGGAGCCGGC 
     4381 TGCCGCGGGA GGAGAGCGCC AACAACCAGT GGGCCCCGCT CAACCCCATC CGCAACCCCA 
     4441 TCGAGCGGCC GGGGGGCCAC AAGGACGTGC TCTACCAGTG CAAGAACTTC ACGCCGCCGC 
     4501 CGCGCAGGGC GGACGAGGCG CTGCCCGGGC CGGCCGGCCA CGCGGCCGTC AGGGAGGATG 
     4561 AGGAGGACGA GGATCTGGGC CGCGGTGAGG AGGACTCCCT GGAGGCGGAG AAGTTCCTCT 
     4621 CACACAAATT CACCAAAGAT CCTGGCCGCT CGCCGGGGAG GCCGGCCCAC TGGGCCTCAG 
     4681 GCCCCAAAGT GGACAACCGC GCGGTCAGGA GCATCAATGA GGCCCGCTAC GCCGGCAAGG 
     4741 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4801 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4861 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4921 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4981 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5041 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5101 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5161 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5221 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5281 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5341 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5401 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5461 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5521 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5581 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5641 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5701 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5761 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5821 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5881 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5941 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6001 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6061 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6121 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6181 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6241 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6301 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6361 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6421 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6481 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6541 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6601 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6661 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6721 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6781 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6841 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6901 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6961 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7021 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7081 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7141 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7201 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7261 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7321 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7381 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7441 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7501 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7561 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7621 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7681 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7741 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7801 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8041 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8101 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8161 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8221 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8281 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8341 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8401 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8461 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8521 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8581 TTTAACGCGA ATTTT
//