LOCUS       RC211919                7917 bp    DNA     circular  SYN 
DEFINITION  IARS2 (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein
ACCESSION   NM_018060
VERSION     NM_018060.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4074..4165 
                     /label="Myc-DDK"  
     misc_feature    4195..4780 
                     /label="PolyA signal"  
     misc_feature    4252..4271 
                     /label="Restriction Enzyme"  
     misc_feature    5056..5728 
                     /label="ColE1"  
     misc_feature    6158..6952 
                     /label="Kan/Neo"  
     misc_feature    6987..7289 
                     /label="SV40 ori"  
     misc_feature    7375..7852 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4056..4062 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4056 
                     /label="IARS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTTGGGGG CTGCGCCCTC GCGGGCCGGG CGCGGCCGCC CTGGCCACTG 
     1081 CCCGAAGTTT GTGGGGGACG CCCCGCCTTC CCTGCAGCCC GGGATGGCAA GGGGCGACGA 
     1141 AGAGGCTTCT GGTGCGGTCG GTCTCCGGGG CCAGTAACCA CCAGCCGAAC TCGAATAGTG 
     1201 GCAGATACCG GGACACGGTG CTGCTGCCGC AGACGAGCTT CCCCATGAAG CTGCTGGGCC 
     1261 GCCAGCAGCC GGACACGGAG CTGGAGATCC AGCAGAAATG TGGATTTTCA GAACTTTATT 
     1321 CATGGCAAAG AGAAAGAAAA GTAAAGACAG AATTTTGCCT TCATGATGGA CCTCCTTATG 
     1381 CAAACGGTGA CCCTCATGTT GGACATGCTT TAAATAAGAT TTTGAAAGAC ATAGCCAATC 
     1441 GATTCCATAT GATGAATGGC TCCAAAATAC ATTTTGTGCC CGGCTGGGAT TGTCATGGGT 
     1501 TGCCCATTGA AATAAAAGTA TTATCAGAAC TTGGTAGAGA AGCTCAGAAT CTTTCAGCTA 
     1561 TGGAAATTAG AAAGAAAGCT AGATCATTTG CTAAAGCAGC CATTGAGAAA CAGAAATCAG 
     1621 CATTTATTCG TTGGGGAATA ATGGCAGATT GGAATAATTG CTACTATACA TTTGATGGGA 
     1681 AGTATGAAGC CAAACAGTTG AGAACTTTTT ACCAAATGTA TGATAAGGGC TTGGTTTATC 
     1741 GATCTTACAA ACCTGTGTTT TGGTCTCCGT CATCTAGGAC TGCATTGGCT GAAGCAGAAC 
     1801 TTGAATATAA TCCTGAGCAT GTCAGTCGTT CAATATATGT AAAATTTCCT CTCTTAAAGC 
     1861 CTTCTCCAAA ATTGGCATCT CTTATAGATG GTTCATCTCC TGTTAGTATT TTGGTCTGGA 
     1921 CCACACAACC TTGGACGATT CCAGCCAATG AAGCTGTTTG CTATATGCCT GAATCAAAGT 
     1981 ATGCTGTTGT GAAATGTTCT AAGTCTGGAG ACCTCTACGT ACTGGCGGCA GATAAAGTAG 
     2041 CATCTGTTGC TTCTACTTTG GAAACAACAT TTGAGACTAT TTCAACACTT TCAGGTGTAG 
     2101 ATTTGGAAAA TGGTACTTGC AGTCATCCAT TAATTCCTGA TAAAGCCTCT CCTCTTTTAC 
     2161 CTGCAAATCA TGTGACCATG GCAAAAGGAA CGGGATTGGT TCACACAGCC CCAGCTCATG 
     2221 GTATGGAAGA CTACGGTGTA GCGTCTCAGC ACAACCTGCC CATGGATTGT CTAGTGGACG 
     2281 AAGATGGAGT TTTCACAGAT GTTGCAGGTC CTGAACTTCA AAACAAGGCT GTCCTTGAAG 
     2341 AGGGAACTGA TGTGGTTATA AAGATGCTTC AGACTGCAAA GAATTTGTTG AAAGAGGAGA 
     2401 AATTGGTGCA TAGCTATCCG TATGACTGGA GGACCAAGAA ACCTGTGGTT ATTCGTGCCA 
     2461 GCAAGCAGTG GTTTATAAAC ATCACGGATA TTAAGACTGC AGCCAAGGAA TTGTTAAAAA 
     2521 AGGTGAAATT TATTCCTGGA TCAGCACTGA ATGGCATGGT TGAAATGATG GACAGGCGGC 
     2581 CATATTGGTG TATATCAAGG CAAAGAGTTT GGGGTGTTCC AATTCCTGTG TTTCATCATA 
     2641 AGACCAAGGA TGAATACTTG ATCAACAGCC AAACCACTGA GCATATTGTT AAACTAGTGG 
     2701 AACAACACGG CAGTGATATC TGGTGGACTC TTCCCCCTGA ACAACTTCTT CCAAAAGAAG 
     2761 TCTTATCTGA GGTTGGTGGC CCTGATGCCT TGGAATATGT GCCAGGTCAG GATATTTTGG 
     2821 ACATCTGGTT TGATAGCGGA ACTTCATGGT CTTATGTTCT TCCAGGTCCT GACCAAAGAG 
     2881 CAGATTTGTA CTTGGAAGGA AAAGACCAGC TCGGGGGTTG GTTTCAGTCA TCCTTATTAA 
     2941 CAAGTGTGGC AGCAAGGAAG AGAGCACCTT ATAAGACAGT GATTGTTCAT GGATTTACCC 
     3001 TTGGAGAAAA GGGAGAAAAG ATGTCCAAGT CTCTTGGGAA TGTCATTCAT CCTGATGTTG 
     3061 TCGTTAATGG AGGACAAGAT CAAAGCAAAG AGCCTCCGTA TGGTGCTGAT GTCCTTTGCT 
     3121 GGTGGGTAGC TGATTCCAAT GTCTTCACCG AAGTTGCAAT TGGCCCATCC GTGCTCAATG 
     3181 CTGCCAGAGA TGATATTAGC AAGCTTAGGA ATACACTTCG CTTTCTTTTG GGAAATGTGG 
     3241 CTGATTTCAA CCCAGAAACA GATTCCATCC CTGTAAACGA TATGTATGTC ATAGACCAGT 
     3301 ACATGCTACA CTTACTGCAG GATTTGGCAA ACAAGATTAC CGAATTATAC AAACAATATG 
     3361 ATTTTGGAAA AGTTGTTCGG CTGTTACGGA CGTTTTATAC CAGAGAGCTC TCTAACTTTT 
     3421 ATTTCAGTAT AATCAAAGAT AGGCTCTATT GTGAAAAGGA AAATGACCCC AAACGACGCT 
     3481 CTTGTCAGAC TGCATTAGTT GAAATTTTGG ATGTAATAGT TCGTTCTTTT GCTCCCATTC 
     3541 TTCCTCACCT GGCTGAAGAG GTGTTCCAGC ACATACCTTA TATTAAAGAG CCCAAGAGTG 
     3601 TTTTCCGTAC TGGGTGGATT AGTACTAGTT CTATCTGGAA AAAGCCCGGG TTGGAAGAAG 
     3661 CTGTGGAGAG TGCGTGTGCA ATGCGAGACT CATTTCTTGG AAGCATCCCT GGCAAAAATG 
     3721 CAGCTGAGTA CAAGGTTATC ACTGTGATAG AACCTGGACT GCTTTTTGAG ATAATAGAGA 
     3781 TGCTGCAGTC TGAAGAGACT TCCAGCACCT CTCAGTTGAA TGAATTAATG ATGGCTTCTG 
     3841 AGTCAACTTT ACTGGCTCAG GAACCACGAG AGATGACTGC AGATGTAATC GAGCTTAAAG 
     3901 GGAAATTCCT CATCAACTTA GAAGGTGGTG ATATTCGTGA AGAGTCTTCC TATAAAGTAA 
     3961 TTGTCATGCC GACTACGAAA GAAAAATGCC CCCGTTGTTG GAAGTATACA GCGGAGTCTT 
     4021 CAGATACACT GTGTCCTCGA TGTGCAGAAG TTGTCAGTGG AAAAACGCGT ACGCGGCCGC 
     4081 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4141 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4201 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4261 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4321 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4381 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4441 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4501 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4561 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4621 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4681 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4741 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4801 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4861 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4921 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4981 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5041 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5161 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5221 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5281 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5341 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5401 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5461 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5521 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5581 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5641 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5701 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5761 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5821 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5881 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5941 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6001 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6061 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6121 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6181 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6241 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6301 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6361 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6421 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6481 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6541 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6601 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6661 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6721 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6781 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6841 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6901 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6961 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7021 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7081 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7141 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7321 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7381 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7441 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7501 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7561 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7621 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7681 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7741 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7801 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7861 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//