LOCUS       RC211905                8382 bp    DNA     circular  SYN 
DEFINITION  NOL8 (Myc-DDK-tagged)-Human nucleolar protein 8 (NOL8), transcript variant 1
ACCESSION   NM_017948
VERSION     NM_017948.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="NOL8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGAAC AGAGAAACGA AGCGCCTTTA TGTGGGTGGC CTTAGCCAGG 
     1081 ACATTTCTGA GGCAGACCTA CAAAATCAGT TCAGCAGATT TGGAGAAGTT TCGGATGTGG 
     1141 AGATCATCAC ACGGAAAGAT GACCAAGGAA ACCCACAGAA AGTTTTTGCA TATATCAACA 
     1201 TCAGTGTAGC AGAAGCGGAC CTGAAAAAAT GTATGTCTGT TTTAAATAAA ACAAAATGGA 
     1261 AAGGTGGAAC ATTACAAATT CAACTAGCAA AAGAAAGCTT TCTGCACAGA TTGGCCCAAG 
     1321 AGAGAGAAGC AGCAAAAGCT AAGAAAGAAG AATCAACAAC AGGTAACGCC AACTTGTTAG 
     1381 AAAAGACAGG AGGAGTGGAT TTCCATATGA AAGCTGTGCC AGGGACAGAA GTGCCAGGGC 
     1441 ATAAGAATTG GGTTGTGAGC AAATTTGGAA GAGTCTTACC TGTTCTTCAC CTTAAAAATC 
     1501 AACATAAACG TAAAATCATC AAATATGATC CCTCAAAATA CTGCCACAAC CTGAAGAAGA 
     1561 TAGGGGAGGA TTTCTCAAAC ACCATTCCTA TATCCAGCCT GACTTGGGAA TTAGAAGGAG 
     1621 GGAATGACCC TATGAGTAAG AAACGGCGAG GAGAGTTCTC TGACTTTCAT GGCCCTCCCA 
     1681 AGAAGATAAT AAAAGTGCAG AAGGATGAGA GTTCCACTGG GTCTCTGGCC ATGAGTACAA 
     1741 GGCCCAGGAG GGTAATAGAG AGACCACCCT TAACACAGCA ACAGGCTGCA CAAAAAAGAA 
     1801 CTTGTGATTC CATTACTCCT TCTAAATCAT CTCCTGTACC TGTTTCTGAT ACTCAGAAAC 
     1861 TTAAAAATCT ACCTTTTAAG ACTTCTGGCT TGGAAACTGC CAAGAAGAGA AACAGCATTT 
     1921 CTGATGATGA TACTGATTCT GAAGATGAAT TGAGAATGAT GATTGCGAAA GAGGAAAACT 
     1981 TACAGAGAAC TACACAACCC TCAATAAATG AATCTGAAAG TGATCCTTTT GAAGTTGTAA 
     2041 GGGATGATTT CAAATCAGGC GTTCACAAAC TGCATTCTTT AATAGGTTTA GGTATCAAAA 
     2101 ATCGTGTCTC TTGCCATGAT AGTGATGATG ATATTATGAG AAATGATCGT GAGTATGACT 
     2161 CAGGAGATAC AGATGAAATT ATTGCGATGA AAAAAAATGT TGCTAAGGTC AAAAACAGTA 
     2221 CAGAATTTTC ACAAATGGAA AAATCTACGA AGAAAACTTC TTTCAAAAAT AGAGAAAACT 
     2281 GTGAGCTTTC TGATCACTGT ATTAAACTAC AAAAAAGAAA AAGCAATGTA GAGTCAGCCC 
     2341 TCAGTCATGG ATTAAAGTCT CTTAATCGTA AATCTCCCTC TCACTCCAGT AGCAGTGAAG 
     2401 ATGCTGATTC TGCATCAGAA TTAGCTGACT CTGAAGGAGG TGAGGAGTAT AATGCCATGA 
     2461 TGAAAAACTG CCTTCGTGTG AATCTCACTT TAGCTGATTT GGAACAATTG GCTGGCAGTG 
     2521 ATCTGAAGGT TCCAAATGAA GATACTAAGA GTGATGGACC AGAAACCACC ACCCAATGCA 
     2581 AGTTTGACAG AGGCTCCAAG AGCCCCAAGA CTCCCACTGG CCTCCGCAGA GGCCGACAGT 
     2641 GTATTCGTCC TGCGGAGATT GTGGCTTCCC TGTTAGAAGG AGAGGAGAAC ACCTGTGGCA 
     2701 AACAGAAACC AAAGGAAAAC AATTTAAAGC CAAAATTTCA GGCTTTCAAG GGAGTAGGCT 
     2761 GTCTATATGA AAAGGAGTCA ATGAAAAAAT CCTTGAAAGA CAGTGTTGCC TCTAACAATA 
     2821 AAGATCAGAA TTCCATGAAA CATGAGGATC CCAGTATCAT ATCCATGGAA GATGGGTCCC 
     2881 CATATGTTAA TGGCTCATTA GGTGAAGTGA CTCCATGCCA ACATGCAAAG AAGGCGAATG 
     2941 GCCCAAACTA TATTCAGCCT CAAAAAAGAC AGACCACTTT TGAAAGCCAG GATCGCAAGG 
     3001 CAGTGTCCCC TAGCAGTTCT GAAAAGAGAA GTAAGAATCC TATTTCTAGG CCATTAGAAG 
     3061 GTAAGAAGTC CTTAAGTCTT AGTGCAAAGA CTCACAACAT AGGCTTTGAC AAAGACAGCT 
     3121 GCCATAGTAC CACAAAGACA GAAGCTTCAC AGGAAGAGCG GTCTGATTCA AGCGGCCTCA 
     3181 CATCTCTCAA GAAATCACCA AAGGTCTCAT CCAAGGACAC TCGGGAAATC AAAACTGATT 
     3241 TCTCACTTTC TATTAGTAAT TCGTCAGATG TGAGTGCTAA AGATAAGCAT GCTGAAGACA 
     3301 ATGAGAAGCG TTTGGCAGCC TTGGAAGCGA GGCAAAAAGC AAAAGAAGTG CAGAAGAAGC 
     3361 TGGTGCATAA TGCTCTGGCA AATTTGGATG GTCATCCAGA GGATAAGCCA ACGCACATCA 
     3421 TCTTCGGTTC TGACAGTGAA TGTGAAACAG AGGAGACATC GACTCAGGAG CAGAGCCATC 
     3481 CAGGAGAGGA ATGGGTGAAA GAGTCTATGG GTAAAACATC AGGGAAGCTG TTTGATAGCA 
     3541 GTGATGATGA CGAATCTGAT TCTGAAGATG ACAGTAATAG GTTCAAAATT AAACCTCAGT 
     3601 TTGAGGGCAG AGCTGGACAG AAGCTCATGG ATTTACAGTC GCACTTTGGC ACCGATGACA 
     3661 GATTCCGCAT GGACTCTCGA TTTCTAGAAA CTGACAGTGA AGAGGAACAG GAAGAGGTAA 
     3721 ATGAAAAGAA AACTGCTGAG GAAGAAGAGC TTGCTGAAGA AAAAAAGAAA GCCCTGAATG 
     3781 TTGTACAAAG TGTTTTGCAA ATCAACTTAA GCAATTCTAC AAACAGAGGA TCAGTAGCTG 
     3841 CTAAGAAATT TAAGGACATC ATACATTATG ATCCAACGAA GCAAGACCAT GCCACTTACG 
     3901 AAAGAAAAAG AGATGATAAG CCAAAAGAAA GTAAAGCAAA ACGAAAAAAG AAAAGGGAGG 
     3961 AAGCTGAGAA ACTACCTGAG GTGTCTAAAG AAATGTATTA TAATATTGCT ATGGATCTGA 
     4021 AAGAAATATT CCAAACTACA AAATATACCA GTGAAAAGGA AGAGGGCACA CCCTGGAATG 
     4081 AGGACTGTGG TAAAGAGAAA CCTGAGGAAA TCCAGGACCC TGCAGCTCTG ACCAGTGACG 
     4141 CTGAGCAGCC CAGCGGGTTC ACGTTCTCTT TTTTTGATTC AGACACTAAA GACATAAAGG 
     4201 AAGAGACCTA CAGAGTTGAA ACAGTGAAAC CTGGAAAGAT TGTCTGGCAG GAAGACCCTC 
     4261 GTTTACAAGA CAGCAGTTCA GAAGAGGAAG ATGTTACTGA AGAAACAGAT CACAGAAACT 
     4321 CCAGTCCTGG AGAAGCATCA TTACTTGAGA AAGAGACCAC TAGATTTTTC TTTTTCTCTA 
     4381 AGAATGATGA ACGACTTCAA GGTTCTGACT TATTCTGGAG AGGAGTAGGA AGTAATATGA 
     4441 GCAGGAACTC TTGGGAGGCC AGAACAACCA ACCTGCGTAT GGATTGTCGA AAGAAACATA 
     4501 AAGACGCAAA AAGGAAAATG AAACCAAAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//