LOCUS       RC211819                8340 bp    DNA     circular  SYN 
DEFINITION  NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2)
ACCESSION   NM_000625
VERSION     NM_000625.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="NOS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTGTCCT TGGAAATTTC TGTTCAAGAC CAAATTCCAC CAGTATGCAA 
     1081 TGAATGGGGA AAAAGACATC AACAACAATG TGGAGAAAGC CCCCTGTGCC ACCTCCAGTC 
     1141 CAGTGACACA GGATGACCTT CAGTATCACA ACCTCAGCAA GCAGCAGAAT GAGTCCCCGC 
     1201 AGCCCCTCGT GGAGACGGGA AAGAAGTCTC CAGAATCTCT GGTCAAGCTG GATGCAACCC 
     1261 CATTGTCCTC CCCACGGCAT GTGAGGATCA AAAACTGGGG CAGCGGGATG ACTTTCCAAG 
     1321 ACACACTTCA CCATAAGGCC AAAGGGATTT TAACTTGCAG GTCCAAATCT TGCCTGGGGT 
     1381 CCATTATGAC TCCCAAAAGT TTGACCAGAG GACCCAGGGA CAAGCCTACC CCTCCAGATG 
     1441 AGCTTCTACC TCAAGCTATC GAATTTGTCA ACAAATATTA CGGCTCCTTC AAAGAGGCAA 
     1501 AAATAGAGGA ACATCTGGCC AGGGTGGAAG CGGTAACAAA GGAGATAGAA ACAACAGGAA 
     1561 CCTACCAACT GACGGGAGAT GAGCTCATCT TCGCCACCAA GCAGGCCTGG CGCAATGCCC 
     1621 CACGCTGCAT TGGGAGGATC CAGTGGTCCA ACCTGCAGGT CTTCGATGCC CGCAGCTGTT 
     1681 CCACTGCCCG GGAAATGTTT GAACACATCT GCAGACACGT GCGTTACTCC ACCAACAATG 
     1741 GCAACATCAG GTCGGCCATC ACCGTGTTCC CCCAGCGGAG TGATGGCAAG CACGACTTCC 
     1801 GGGTGTGGAA TGCTCAGCTC ATCCGCTATG CTGGCTACCA GATGCCAGAT GGCAGCATCA 
     1861 GAGGGGACCC TGCCAACGTG GAATTCACTC AGCTGTGCAT CGACCTGGGC TGGAAGCCCA 
     1921 AGTACGGCCG CTTCGATGTG GTCCCCCTGG TCCTGCAGGC CAATGGCCGT GACCCTGAGC 
     1981 TCTTCGAAAT CCCACCTGAC CTTGTGCTTG AGGTGGCCAT GGAACATCCC AAATACGAGT 
     2041 GGTTTCGGGA ACTGGAGCTA AAGTGGTACG CCCTGCCTGC AGTGGCCAAC ATGCTGCTTG 
     2101 AGGTGGGCGG CCTGGAGTTC CCAGGGTGCC CCTTCAATGG CTGGTACATG GGCACAGAGA 
     2161 TCGGAGTCCG GGACTTCTGT GACGTCCAGC GCTACAACAT CCTGGAGGAA GTGGGCAGGA 
     2221 GAATGGGCCT GGAAACGCAC AAGCTGGCCT CGCTCTGGAA AGACCAGGCT GTCGTTGAGA 
     2281 TCAACATTGC TGTGCTCCAT AGTTTCCAGA AGCAGAATGT GACCATCATG GACCACCACT 
     2341 CGGCTGCAGA ATCCTTCATG AAGTACATGC AGAATGAATA CCGGTCCCGT GGGGGCTGCC 
     2401 CGGCAGACTG GATTTGGCTG GTCCCTCCCA TGTCTGGGAG CATCACCCCC GTGTTTCACC 
     2461 AGGAGATGCT GAACTACGTC CTGTCCCCTT TCTACTACTA TCAGGTAGAG GCCTGGAAAA 
     2521 CCCATGTCTG GCAGGACGAG AAGCGGAGAC CCAAGAGAAG AGAGATTCCA TTGAAAGTCT 
     2581 TGGTCAAAGC TGTGCTCTTT GCCTGTATGC TGATGCGCAA GACAATGGCG TCCCGAGTCA 
     2641 GAGTCACCAT CCTCTTTGCG ACAGAGACAG GAAAATCAGA GGCGCTGGCC TGGGACCTGG 
     2701 GGGCCTTATT CAGCTGTGCC TTCAACCCCA AGGTTGTCTG CATGGATAAG TACAGGCTGA 
     2761 GCTGCCTGGA GGAGGAACGG CTGCTGTTGG TGGTGACCAG TACGTTTGGC AATGGAGACT 
     2821 GCCCTGGCAA TGGAGAGAAA CTGAAGAAAT CGCTCTTCAT GCTGAAAGAG CTCAACAACA 
     2881 AATTCAGGTA CGCTGTGTTT GGCCTCGGCT CCAGCATGTA CCCTCGGTTC TGCGCCTTTG 
     2941 CTCATGACAT TGATCAGAAG CTGTCCCACC TGGGGGCCTC TCAGCTCACC CCGATGGGAG 
     3001 AAGGGGATGA GCTCAGTGGG CAGGAGGACG CCTTCCGCAG CTGGGCCGTG CAAACCTTCA 
     3061 AGGCAGCCTG TGAGACGTTT GATGTCCGAG GCAAACAGCA CATTCAGATC CCCAAGCTCT 
     3121 ACACCTCCAA TGTGACCTGG GACCCGCACC ACTACAGGCT CGTGCAGGAC TCACAGCCTT 
     3181 TGGACCTCAG CAAAGCCCTC AGCAGCATGC ATGCCAAGAA CGTGTTCACC ATGAGGCTCA 
     3241 AATCTCGGCA GAATCTACAA AGTCCGACAT CCAGCCGTGC CACCATCCTG GTGGAACTCT 
     3301 CCTGTGAGGA TGGCCAAGGC CTGAACTACC TGCCGGGGGA GCACCTTGGG GTTTGCCCAG 
     3361 GCAACCAGCC GGCCCTGGTC CAAGGTATCC TGGAGCGAGT GGTGGATGGC CCCACACCCC 
     3421 ACCAGACAGT GCGCCTGGAG GCCCTGGATG AGAGTGGCAG CTACTGGGTC AGTGACAAGA 
     3481 GGCTGCCCCC CTGCTCACTC AGCCAGGCCC TCACCTACTT CCTGGACATC ACCACACCCC 
     3541 CAACCCAGCT GCTGCTCCAA AAGCTGGCCC AGGTGGCCAC AGAAGAGCCT GAGAGACAGA 
     3601 GGCTGGAGGC CCTGTGCCAG CCCTCAGAGT ACAGCAAGTG GAAGTTCACC AACAGCCCCA 
     3661 CATTCCTGGA GGTGCTAGAG GAGTTCCCGT CCCTGCGGGT GTCTGCTGGC TTCCTGCTTT 
     3721 CCCAGCTCCC CATTCTGAAG CCCAGGTTCT ACTCCATCAG CTCCTCCCGG GATCACATGC 
     3781 CCACAGAGAT CCACCTGACT GTGGCCGTGG TCACCTACCA CACCCGAGAT GGCCAGGGTC 
     3841 CCCTGCACCA CGGCGTCTGC AGCACATGGC TCAACAGCCT GAAGCCCCAA GACCCAGTGC 
     3901 CCTGCTTTGT GCGGAATGCC AGCGGCTTCC ACCTCCCCGA GGATCCCTCC CATCCTTGCA 
     3961 TCCTCATCGG GCCTGGCACA GGCATCGCGC CCTTCCGCAG TTTCTGGCAG CAACGGCTCC 
     4021 ATGACTCCCA GCACAAGGGA GTGCGGGGAG GCCGCATGAC CTTGGTGTTT GGGTGCCGCC 
     4081 GCCCAGATGA GGACCACATC TACCAGGAGG AGATGCTGGA GATGGCCCAG AAGGGGGTGC 
     4141 TGCATGCGGT GCACACAGCC TATTCCCGCC TGCCTGGCAA GCCCAAGGTC TATGTTCAGG 
     4201 ACATCCTGCG GCAGCAGCTG GCCAGCGAGG TGCTCCGTGT GCTCCACAAG GAGCCAGGCC 
     4261 ACCTCTATGT TTGCGGGGAT GTGCGCATGG CCCGGGACGT GGCCCACACC CTGAAGCAGC 
     4321 TGGTGGCTGC CAAGCTGAAA TTGAATGAGG AGCAGGTCGA GGACTATTTC TTTCAGCTCA 
     4381 AGAGCCAGAA GCGCTATCAC GAAGATATCT TTGGTGCTGT ATTTCCTTAC GAGGCGAAGA 
     4441 AGGACAGGGT GGCGGTGCAG CCCAGCAGCC TGGAGATGTC AGCGCTCACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//