LOCUS       RC211815                8634 bp    DNA     circular  SYN 
DEFINITION  ADCY8 (Myc-DDK-tagged)-Human adenylate cyclase 8 (brain) (ADCY8)
ACCESSION   NM_001115
VERSION     NM_001115.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="ADCY8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTCC GATGTGCGCT GCCTTACAGG CAGCGAGGAA CTCTACACCA 
     1081 TCCACCCGAC GCCCCCGGCC GGCGACGGCA GGAGCGCCTC CCGGCCGCAG CGGCTGCTGT 
     1141 GGCAGACGGC GGTGCGACAC ATCACGGAGC AGCGCTTCAT TCACGGGCAC CGGGGAGGCA 
     1201 GCGGCAGCGG GAGTGGAGGC TCGGGCAAAG CCTCGGACCC TGCGGGCGGC GGCCCCAACC 
     1261 ACCACACGCC GCAGCTGTCA GGCGACTCGG CGCTGCCCCT CTACTCGCTG GGCCCGGGAG 
     1321 AGCGAGCGCA CAGCACCTGC GGCACCAAAG TCTTCCCGGA ACGCAGCGGG AGCGGCAGTG 
     1381 CCAGCGGCAG CGGAGGCGGG GGCGACCTGG GCTTCCTGCA CCTTGACTGT GCCCCTAGCA 
     1441 ACTCGGATTT CTTTCTTAAT GGGGGCTATA GCTACCGAGG GGTCATTTTC CCCACCCTGC 
     1501 GCAACTCCTT CAAATCTCGG GATTTGGAAC GCCTCTACCA GCGCTATTTC TTGGGCCAAA 
     1561 GGCGCAAATC GGAAGTGGTG ATGAACGTGC TGGACGTGCT GACCAAACTC ACTCTCTTGG 
     1621 TCCTACACTT GAGCCTGGCC TCGGCCCCCA TGGACCCGCT CAAGGGCATC CTGCTGGGCT 
     1681 TCTTCACCGG CATTGAGGTA GTGATCTGCG CCCTGGTGGT GGTCAGGAAG GACACCACCT 
     1741 CCCACACGTA CCTGCAGTAC AGCGGCGTGG TCACCTGGGT GGCCATGACC ACCCAGATCC 
     1801 TGGCAGCAGG CCTCGGCTAC GGGCTCCTGG GCGACGGCAT AGGCTACGTG CTCTTCACGC 
     1861 TCTTCGCCAC CTACAGTATG CTGCCGCTGC CGCTCACCTG GGCCATCCTG GCCGGCCTGG 
     1921 GCACCTCGCT GCTGCAGGTC ATCCTCCAAG TGGTCATACC CCGGCTGGCG GTCATTTCCA 
     1981 TCAACCAGGT TGTGGCCCAG GCAGTGCTAT TCATGTGTAT GAACACAGCT GGAATCTTCA 
     2041 TCAGTTACCT GTCAGACCGT GCCCAGCGCC AAGCTTTCCT GGAGACTCGG AGGTGTGTGG 
     2101 AGGCCAGGCT GCGCCTGGAG ACAGAGAACC AAAGACAGGA GCGGCTCGTG CTTTCTGTGC 
     2161 TCCCCCGGTT TGTTGTCCTG GAAATGATCA ACGACATGAC CAATGTGGAA GATGAGCACC 
     2221 TGCAGCACCA GTTCCATCGG ATCTACATCC ATCGCTATGA GAACGTCAGT ATTCTTTTTG 
     2281 CAGATGTTAA AGGATTTACC AACCTCTCCA CGACCTTGTC TGCTCAGGAG CTGGTCAGGA 
     2341 TGCTCAACGA GCTCTTTGCC AGATTTGATC GACTGGCCCA TGAGCATCAC TGCCTTCGTA 
     2401 TTAAAATCCT GGGGGACTGC TACTACTGCG TGTCTGGACT TCCTGAGCCC CGCCAGGACC 
     2461 ATGCCCACTG CTGTGTTGAA ATGGGTCTCA GCATGATCAA AACCATCAGG TATGTGCGGT 
     2521 CAAGGACAAA ACACGATGTT GACATGAGGA TTGGAATCCA CTCCGGCTCG GTGCTGTGCG 
     2581 GTGTTTTGGG ACTAAGGAAG TGGCAGTTTG ATGTCTGGTC TTGGGATGTG GATATTGCAA 
     2641 ACAAACTCGA ATCTGGAGGA ATCCCTGGGA GGATTCACAT TTCCAAAGCC ACGCTGGACT 
     2701 GTCTCAACGG TGACTATAAC GTGGAAGAGG GCCATGGTAA AGAGAGGAAT GAATTCCTGA 
     2761 GGAAGCATAA TATCGAAACT TACTTAATTA AGCAGCCTGA GGACAGTCTG CTGTCCTTGC 
     2821 CTGAAGATAT CGTCAAGGAG TCAGTGAGCT CCTCAGACCG GAGAAACAGT GGGGCCACAT 
     2881 TCACTGAAGG ATCCTGGAGC CCTGAACTGC CCTTTGATAA TATCGTGGGG AAACAGAATA 
     2941 CTCTGGCTGC CCTAACAAGA AATTCAATAA ATCTGCTTCC AAACCATCTT GCACAAGCTT 
     3001 TGCATGTCCA GTCTGGGCCT GAGGAAATTA ACAAGAGAAT AGAACATACC ATCGACTTGC 
     3061 GGAGTGGCGA TAAATTGAGA AGAGAGCATA TCAAGCCATT CTCACTGATG TTTAAAGACT 
     3121 CCAGCCTGGA GCACAAGTAT TCTCAAATGA GGGATGAAGT GTTCAAGTCA AACTTGGTCT 
     3181 GTGCATTTAT CGTTCTTCTA TTTATCACGG CAATACAAAG TTTGCTTCCT TCTTCAAGAG 
     3241 TGATGCCAAT GACCATCCAG TTCTCCATTC TGATTATGCT GCACTCGGCT CTGGTCCTCA 
     3301 TCACCACAGC AGAGGATTAT AAATGTTTGC CCCTCATCCT CCGGAAAACT TGCTGTTGGA 
     3361 TTAATGAGAC CTATTTGGCC CGGAACGTCA TCATCTTTGC ATCCATTTTG ATTAATTTCC 
     3421 TGGGTGCCAT CTTAAATATC CTGTGGTGTG ATTTTGACAA GTCGATACCC TTGAAGAACC 
     3481 TGACTTTCAA TTCCTCAGCT GTGTTTACAG ATATCTGCTC CTACCCAGAG TACTTTGTCT 
     3541 TCACGGGGGT GTTGGCCATG GTGACCTGTG CAGTTTTCCT CCGGCTGAAC TCCGTCCTGA 
     3601 AGCTGGCAGT GCTGCTGATC ATGATTGCCA TCTATGCCCT GCTCACTGAG ACCGTCTACG 
     3661 TAGGCCTCTT TCTGCGTTAT GACAACCTCA ACCACAGTGG AGAAGATTTC CTGGGGACCA 
     3721 AGGAGGTATC ACTGCTACTG ATGGCCATGT TCCTCCTGGC TGTGTTCTAC CATGGACAGC 
     3781 AGCTGGAGTA CACAGCCCGC CTGGACTTCC TTTGGCGAGT ACAGGCCAAA GAGGAGATCA 
     3841 ATGAGATGAA GGAGCTGAGG GAACACAATG AGAACATGCT CCGGAATATC TTACCCAGCC 
     3901 ATGTGGCCCG CCATTTCCTA GAGAAGGACC GAGACAATGA GGAGCTGTAT TCTCAATCCT 
     3961 ATGATGCTGT TGGGGTGATG TTTGCCTCCA TCCCAGGATT TGCGGACTTT TACTCTCAGA 
     4021 CTGAAATGAA TAACCAGGGA GTGGAATGCC TGCGCTTGCT CAATGAGATC ATTGCTGACT 
     4081 TCGATGAGTT GCTTGGTGAA GACCGATTTC AAGACATTGA AAAGATTAAG ACCATTGGCA 
     4141 GCACCTACAT GGCCGTGTCA GGCCTGTCAC CTGAAAAACA GCAATGTGAA GACAAGTGGG 
     4201 GACATTTGTG TGCTCTGGCT GACTTCTCAC TCGCCCTGAC AGAAAGCATA CAGGAGATCA 
     4261 ACAAGCATTC ATTCAACAAT TTTGAACTCC GGATTGGCAT CAGCCACGGC TCAGTGGTAG 
     4321 CTGGCGTTAT CGGCGCTAAG AAACCACAGT ATGACATTTG GGGCAAAACT GTGAACCTGG 
     4381 CAAGCCGAAT GGACAGCACG GGGGTTAGTG GCCGGATCCA AGTCCCAGAG GAGACCTATC 
     4441 TCATCCTGAA GGACCAGGGC TTTGCCTTTG ATTACCGAGG GGAGATCTAT GTGAAGGGTA 
     4501 TCAGTGAACA GGAAGGAAAA ATCAAAACGT ACTTTCTTCT GGGAAGAGTC CAACCCAACC 
     4561 CATTCATCTT GCCCCCAAGA AGACTGCCTG GGCAGTACTC CCTGGCCGCG GTTGTCCTGG 
     4621 GACTTGTCCA GTCCCTCAAT AGGCAAAGGC AGAAGCAGCT ACTCAATGAG AACAACAACA 
     4681 CAGGAATCAT CAAGGGTCAT TACAACCGGC GGACTTTGTT GTCACCCAGC GGCACAGAGC 
     4741 CTGGAGCCCA GGCTGAAGGC ACCGACAAAT CTGATTTGCC AACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//