LOCUS       RC211776L2                9408 bp    DNA     circular  SYN 
DEFINITION  CAST (Myc-DDK-tagged)-Human calpastatin (CAST), transcript variant 1
ACCESSION   NM_001750
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7699..7879 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8607..9266 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7927..8546 
                     /label="ColE1"  
     misc_feature    6574..6594 
                     /label="LR50"  
     misc_feature    5714..6439 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6583..7174 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5693..5699 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5693 
                     /label="CAST"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCCCAGCCC GGCCAGAAGC CCGCCGCCTC 
     3361 CCCGCGGCCC CGGCGAGCAG CCGCCGCCCG CCGCACCCAT GAGCATGTCA GTGAAAAAAC 
     3421 CAGTGAATCG CCTTCCAAAC CAGGAGAAAA GAAAGGATCA GATGAGAAAA AAGCAGCAAG 
     3481 CCTCGGCAGC AGTCAATCCT CCAGAACCTA TGCTGGTGGA ACAGCCTCGG CCACCAAGGT 
     3541 GTCAGCTTCC TCTGGTGCAA CCAGCAAGTC TTCCAGTATG AATCCCACAG AAACCAAGGC 
     3601 CATTCCAGTC AGCCAACAGA TGGAAGGACC ACATCTTCCT AACAAGAAAA AACACAAAAA 
     3661 ACAGGCTGTA AAAACAGAAC CTGAGAAGAA GTCACAGTCA ACCAAGCTGT CTGTGGTTCA 
     3721 TGAGAAAAAA TCCCAAGAAG GAAAGCCAAA AGAACACACA GAGCCAAAAA GCCTACCCAA 
     3781 GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA AAAGCAGTTT CCAGATCAGC 
     3841 TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT AAACCACAAG ACATGATTTC 
     3901 TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT GGCAAGCCGG GTGACAAGAA 
     3961 AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA TCTAAACCGG ATAAACCATC 
     4021 GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA GATACTTTAG GAGGACCTGA 
     4081 AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA GTTTCAGATC CAATGAGTTC 
     4141 CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT CCTCCAAAAT ATAGGGAACT 
     4201 ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC TCTTCGAAAC CCATAGGGCC 
     4261 AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG 
     4321 AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAT CAGGGACAGT 
     4381 CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA 
     4441 TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA 
     4501 CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG 
     4561 TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA 
     4621 TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT 
     4681 CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC 
     4741 AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG 
     4801 TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC 
     4861 AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC 
     4921 AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA 
     4981 AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA 
     5041 TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA 
     5101 AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT 
     5161 TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC 
     5221 TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA 
     5281 TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA 
     5341 CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG 
     5401 AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA 
     5461 CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA 
     5521 AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC 
     5581 ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA 
     5641 TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA 
     5701 CACGCGTACG CGGCCGCTCG AGATGAGCGG GGGCGAGGAG CTGTTCGCCG GCATCGTGCC 
     5761 CGTGCTGATC GAGCTGGACG GCGACGTGCA CGGCCACAAG TTCAGCGTGC GCGGCGAGGG 
     5821 CGAGGGCGAC GCCGACTACG GCAAGCTGGA GATCAAGTTC ATCTGCACCA CCGGCAAGCT 
     5881 GCCCGTGCCC TGGCCCACCC TGGTGACCAC CCTCTGCTAC GGCATCCAGT GCTTCGCCCG 
     5941 CTACCCCGAG CACATGAAGA TGAACGACTT CTTCAAGAGC GCCATGCCCG AGGGCTACAT 
     6001 CCAGGAGCGC ACCATCCAGT TCCAGGACGA CGGCAAGTAC AAGACCCGCG GCGAGGTGAA 
     6061 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCAAGGACT TCAAGGAGGA 
     6121 CGGCAACATC CTGGGCCACA AGCTGGAGTA CAGCTTCAAC AGCCACAACG TGTACATCCG 
     6181 CCCCGACAAG GCCAACAACG GCCTGGAGGC TAACTTCAAG ACCCGCCACA ACATCGAGGG 
     6241 CGGCGGCGTG CAGCTGGCCG ACCACTACCA GACCAACGTG CCCCTGGGCG ACGGCCCCGT 
     6301 GCTGATCCCC ATCAACCACT ACCTGAGCAC TCAGACCAAG ATCAGCAAGG ACCGCAACGA 
     6361 GGCCCGCGAC CACATGGTGC TCCTGGAGTC CTTCAGCGCC TGCTGCCACA CCCACGGCAT 
     6421 GGACGAGCTG TACAGGTCCG GACTCAGATA AGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6481 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6541 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6601 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6661 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6721 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6781 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6841 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     6901 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     6961 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     7021 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     7081 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     7141 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     7201 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     7261 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     7321 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     7381 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     7441 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7501 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7561 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7621 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7681 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7741 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7801 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7861 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     7921 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     7981 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     8041 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     8101 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     8161 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     8221 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     8281 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     8341 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     8401 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     8461 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8521 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8581 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8641 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8701 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8761 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8821 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     8881 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     8941 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     9001 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     9061 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     9121 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     9181 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     9241 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     9301 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9361 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//