LOCUS       RC211772                8004 bp    DNA     circular  SYN 
DEFINITION  ATP4A (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
ACCESSION   NM_000704
VERSION     NM_000704.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4159..4250 
                     /label="Myc-DDK"  
     misc_feature    4280..4865 
                     /label="PolyA signal"  
     misc_feature    4337..4356 
                     /label="Restriction Enzyme"  
     misc_feature    5141..5813 
                     /label="ColE1"  
     misc_feature    6243..7037 
                     /label="Kan/Neo"  
     misc_feature    7072..7374 
                     /label="SV40 ori"  
     misc_feature    7460..7937 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4134..4141 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4134 
                     /label="ATP4A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGGCC GAGAACTATG AGCTCTACTC GGTGGAGCTG GGTCCTGGCC 
     1081 CTGGCGGGGA CATGGCTGCC AAGATGAGCA AGAAGAAGAA GGCGGGTGGC GGGGGTGGCA 
     1141 AGAGGAAGGA GAAGCTGGAG AACATGAAGA AGGAGATGGA GATTAACGAC CACCAGCTGT 
     1201 CAGTGGCGGA GCTGGAACAG AAATACCAGA CCAGTGCCAC CAAGGGCCTC TCTGCGAGCC 
     1261 TGGCTGCTGA GCTGCTGCTG CGGGATGGGC CCAACGCACT GCGGCCACCA CGGGGCACCC 
     1321 CAGAGTACGT CAAGTTTGCG AGGCAGCTGG CCGGGGGCCT GCAGTGCCTC ATGTGGGTTG 
     1381 CCGCCGCCAT CTGCCTCATC GCCTTTGCCA TCCAGGCTAG TGAGGGGGAC CTCACCACCG 
     1441 ACGACAATCT GTACCTGGCA ATCGCTCTCA TTGCTGTGGT TGTCGTCACC GGCTGCTTTG 
     1501 GCTACTACCA GGAATTCAAG AGCACCAACA TCATCGCCAG CTTTAAGAAC CTTGTGCCAC 
     1561 AGCAAGCCAC TGTCATCCGC GATGGAGACA AATTCCAGAT CAACGCTGAC CAACTGGTGG 
     1621 TGGGCGACCT GGTGGAGATG AAAGGTGGGG ACAGAGTGCC CGCCGACATC CGCATCCTGG 
     1681 CGGCCCAGGG CTGCAAGGTG GACAACTCCT CGCTGACAGG GGAGTCTGAG CCACAGACCC 
     1741 GCTCACCCGA GTGCACGCAC GAGAGCCCTC TGGAGACCCG CAACATCGCC TTCTTCTCCA 
     1801 CCATGTGCCT TGAGGGCACC GTGCAGGGCC TGGTGGTGAA CACGGGCGAC CGCACCATCA 
     1861 TTGGGCGCAT CGCATCGCTG GCGTCGGGGG TGGAAAACGA GAAGACACCC ATCGCTATCG 
     1921 AGATCGAGCA TTTTGTGGAC ATCATCGCGG GCCTGGCCAT TCTCTTCGGT GCCACATTTT 
     1981 TTATTGTGGC CATGTGCATT GGCTACACCT TCCTGCGGGC CATGGTCTTC TTCATGGCCA 
     2041 TCGTGGTGGC CTATGTGCCT GAGGGGCTGC TGGCCACTGT CACAGTCTGC CTGTCCCTGA 
     2101 CAGCCAAGCG CCTGGCCAGT AAGAACTGCG TGGTCAAGAA CCTGGAGGCG GTGGAGACAT 
     2161 TGGGCTCCAC TTCGGTGATC TGCTCGGACA AGACAGGGAC TCTCACTCAG AACCGCATGA 
     2221 CTGTGTCCCA TCTGTGGTTT GACAACCACA TCCACACAGC TGACACCACG GAAGACCAGT 
     2281 CAGGGCAGAC GTTTGACCAG TCCTCGGAGA CGTGGCGGGC GCTGTGCCGG GTGCTCACCC 
     2341 TGTGCAACCG CGCCGCCTTC AAGTCCGGCC AGGATGCAGT GCCTGTGCCC AAGCGCATCG 
     2401 TGATTGGAGA CGCATCGGAG ACGGCGCTGC TCAAGTTCTC GGAGCTGACG CTGGGCAACG 
     2461 CCATGGGCTA CCGGGACCGC TTCCCAAAAG TCTGCGAGAT ACCCTTCAAC TCCACCAACA 
     2521 AGTTCCAGCT GTCCATCCAT ACGCTGGAGG ACCCGCGGGA CCCGCGACAC TTGCTGGTGA 
     2581 TGAAGGGCGC CCCCGAGCGC GTGCTGGAGC GCTGCAGCTC CATCCTTATC AAGGGCCAGG 
     2641 AGCTGCCGCT GGACGAGCAG TGGCGCGAGG CCTTCCAGAC CGCCTACCTC AGCCTGGGAG 
     2701 GCCTGGGCGA ACGCGTGCTC GGCTTCTGCC AGCTCTACCT GAATGAGAAG GACTACCCGC 
     2761 CTGGCTATGC CTTCGACGTA GAGGCCATGA ACTTTCCATC TAGCGGCCTC TGCTTTGCGG 
     2821 GACTTGTATC CATGATTGAC CCACCCCGGG CCACCGTCCC TGATGCTGTG CTCAAGTGTC 
     2881 GCACCGCAGG CATCCGGGTG ATCATGGTAA CGGGTGACCA CCCCATCACC GCCAAGGCCA 
     2941 TTGCAGCCAG TGTGGGCATC ATCTCGGAAG GCAGCGAGAC AGTGGAGGAC ATCGCTGCCC 
     3001 GCCTCCGTGT GCCCGTAGAC CAGGTTAATC GCAAGGATGC CCGTGCCTGT GTGATCAATG 
     3061 GCATGCAGCT GAAGGACATG GACCCATCGG AACTGGTCGA GGCCCTGCGC ACCCACCCCG 
     3121 AGATGGTGTT TGCGCGCACC AGCCCCCAGC AGAAGCTGGT GATCGTGGAG AGCTGCCAGC 
     3181 GGCTGGGTGC GATTGTGGCC GTCACGGGGG ATGGTGTGAA TGACTCCCCA GCTCTGAAGA 
     3241 AGGCAGACAT CGGAGTAGCC ATGGGCATCG CTGGCTCAGA TGCTGCCAAA AATGCAGCTG 
     3301 ACATGATCCT GCTGGATGAC AACTTTGCCT CCATTGTGAC AGGCGTGGAG CAGGGTCGAC 
     3361 TGATCTTCGA CAACCTGAAG AAGTCTATTG CCTACACATT GACCAAGAAC ATCCCAGAGC 
     3421 TGACACCCTA CCTCATCTAC ATCACCGTCA GCGTGCCCCT GCCCCTCGGG TGCATCACCA 
     3481 TCCTCTTCAT CGAACTCTGC ACTGACATTT TCCCATCTGT GTCCCTGGCA TATGAAAAGG 
     3541 CCGAGAGTGA CATCATGCAC CTGCGTCCAC GCAACCCAAA GCGTGACAGA TTGGTCAACG 
     3601 AGCCCCTGGC TGCCTACTCC TACTTCCAGA TTGGTGCCAT TCAGTCCTTT GCTGGCTTCA 
     3661 CTGACTACTT CACGGCAATG GCCCAGGAGG GCTGGTTCCC ACTGCTGTGC GTGGGGCTGC 
     3721 GGGCGCAGTG GGAGGACCAC CACCTACAAG ATCTGCAGGA CAGCTACGGC CAGGAGTGGA 
     3781 CATTCGGGCA GCGCCTGTAC CAGCAGTACA CCTGCTACAC CGTGTTCTTC ATCAGCATTG 
     3841 AGGTGTGCCA GATCGCCGAT GTCCTCATCC GCAAGACGCG CCGTCTCTCT GCCTTCCAGC 
     3901 AAGGCTTCTT CAGGAATAAG ATCCTGGTGA TCGCCATCGT GTTCCAGGTC TGCATCGGCT 
     3961 GCTTCCTGTG CTACTGCCCC GGCATGCCCA ACATCTTCAA CTTCATGCCC ATTCGGTTCC 
     4021 AGTGGTGGCT GGTCCCCCTG CCCTACGGCA TCCTCATCTT CGTCTATGAT GAGATCCGGA 
     4081 AGCTTGGAGT TCGCTGTTGC CCAGGGAGCT GGTGGGACCA GGAACTCTAC TATAGCGGAC 
     4141 CGAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4201 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4261 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4321 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4381 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4441 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4501 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4561 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4621 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4681 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4741 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4801 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4861 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4921 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4981 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5041 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5101 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5161 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5221 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5281 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5341 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5401 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5461 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5521 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5581 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5641 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5701 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5761 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5821 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5881 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5941 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6001 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6061 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6121 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6181 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6241 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6301 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6361 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6421 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6481 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6541 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6601 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6661 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6721 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6781 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6841 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6901 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6961 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7021 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7081 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7141 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7201 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7381 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7441 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7501 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7561 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7621 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7681 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7741 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7801 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7861 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7921 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7981 TAACAAAAAT TTAACGCGAA TTTT
//