LOCUS       RC211706                7584 bp    DNA     circular  SYN 
DEFINITION  ACTN3 (Myc-DDK-tagged)-Human actinin, alpha 3 (ACTN3)
ACCESSION   NM_001104
VERSION     NM_001104.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3741..3832 
                     /label="Myc-DDK"  
     misc_feature    3862..4447 
                     /label="PolyA signal"  
     misc_feature    3919..3938 
                     /label="Restriction Enzyme"  
     misc_feature    4723..5395 
                     /label="ColE1"  
     misc_feature    5825..6619 
                     /label="Kan/Neo"  
     misc_feature    6654..6956 
                     /label="SV40 ori"  
     misc_feature    7042..7519 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3723..3729 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3723 
                     /label="ACTN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATGGTT ATGCAGCCCG AGGGTCTGGG GGCCGGGGAG GGGCGCTTTG 
     1081 CGGGCGGCGG CGGGGGCGGC GAGTACATGG AACAGGAGGA GGACTGGGAC CGCGACCTGC 
     1141 TGCTGGACCC GGCCTGGGAG AAGCAGCAGC GGAAAACCTT CACTGCCTGG TGCAACTCAC 
     1201 ACCTGCGCAA GGCAGGCACC CAGATCGAGA ACATCGAGGA AGATTTCCGC AATGGCCTCA 
     1261 AACTCATGCT GCTCCTGGAG GTCATTTCAG GTGAGAGGCT GCCTAGGCCA GATAAAGGCA 
     1321 AGATGCGCTT CCACAAAATC GCCAACGTTA ACAAGGCCCT GGACTTCATT GCCAGCAAGG 
     1381 GGGTTAAACT GGTGTCCATT GGTGCTGAAG AGATTGTTGA CGGGAACCTG AAGATGACCC 
     1441 TGGGCATGAT CTGGACCATC ATCCTTCGCT TCGCCATCCA GGACATCTCT GTGGAAGAAA 
     1501 CCTCAGCCAA GGAAGGCTTG CTTCTGTGGT GCCAGAGGAA GACAGCACCG TACCGCAACG 
     1561 TCAACGTGCA GAACTTCCAC ACCAGCTGGA AGGATGGCCT GGCCCTCTGT GCCCTCATCC 
     1621 ACCGACACCG CCCTGACCTC ATCGACTACG CCAAACTGCG AAAGGATGAC CCCATCGGAA 
     1681 ACCTGAACAC TGCCTTTGAG GTGGCAGAGA AATACCTGGA CATCCCCAAG ATGTTGGATG 
     1741 CAGAAGACAT TGTGAACACC CCAAAGCCGG ATGAGAAGGC CATCATGACC TATGTGTCCT 
     1801 GCTTCTACCA TGCCTTTGCC GGGGCTGAGC AGGCAGAGAC AGCTGCCAAC AGGATCTGCA 
     1861 AGGTGCTGGC AGTGAACCAG GAAAACGAGA AGCTGATGGA GGAGTATGAG AAGCTTGCCA 
     1921 GTGAGCTGCT GGAGTGGATC CGCCGCACTG TCCCATGGCT GGAGAACCGT GTGGGTGAGC 
     1981 CCAGCATGAG TGCCATGCAG CGCAAACTAG AGGACTTTCG GGACTACCGG CGTCTGCACA 
     2041 AGCCGCCCCG CATTCAGGAA AAGTGCCAGC TGGAGATCAA CTTCAACACA CTGCAGACCA 
     2101 AGTTGCGGCT CAGCCACCGG CCTGCCTTCA TGCCCTCCGA GGGCAAGCTG GTCTCGGACA 
     2161 TCGCCAACGC CTGGCGGGGG CTGGAGCAGG TGGAAAAGGG CTATGAGGAC TGGCTGCTCT 
     2221 CGGAGATCCG GCGCCTGCAG CGACTCCAGC ACCTGGCTGA GAAGTTCCGG CAGAAGGCCT 
     2281 CCCTGCACGA AGCCTGGACC CGGGGAAAGG AGGAGATGCT GAGCCAGCGC GACTACGATT 
     2341 CGGCTTTGCT ACAGGAGGTG CGGGCGTTGC TGCGGCGCCA CGAGGCCTTT GAGAGCGACC 
     2401 TGGCGGCGCA CCAGGACCGC GTGGAGCACA TTGCCGCGCT GGCCCAGGAG CTCAATGAGC 
     2461 TGGACTACCA CGAGGCAGCC TCAGTGAATA GCCGCTGCCA GGCCATCTGC GATCAGTGGG 
     2521 ACAACCTGGG CACCCTGACC CAGAAGAGGC GGGATGCGCT AGAGCGGATG GAGAAGCTCC 
     2581 TGGAGACCAT TGACCAGCTG CAACTGGAGT TTGCCCGGCG GGCCGCGCCC TTCAACAACT 
     2641 GGCTGGATGG TGCCGTGGAG GACCTGCAGG ACGTGTGGCT GGTACACTCT GTGGAGGAGA 
     2701 CCCAGAGCCT GCTGACAGCG CACGATCAGT TCAAGGCAAC ACTGCCCGAG GCTGACCGAG 
     2761 AGCGAGGTGC CATCATGGGC ATCCAGGGTG AGATCCAGAA GATCTGCCAG ACGTATGGGC 
     2821 TGCGGCCCTG CTCCACCAAT CCCTACATCA CCCTCAGCCC GCAGGACATC AACACCAAGT 
     2881 GGGATATGGT CCGAAAGCTG GTGCCCAGCC GTGACCAGAC ACTGCAGGAG GAGCTGGCAC 
     2941 GGCAGCAGGT AAACGAGAGG CTCCGGCGAC AGTTTGCGGC CCAGGCCAAT GCCATTGGAC 
     3001 CCTGGATCCA GGCGAAGGTG GAGGAAGTGG GGCGGCTGGC AGCAGGGCTA GCTGGCTCTC 
     3061 TGGAGGAGCA GATGGCTGGG CTACGGCAGC AGGAGCAGAA CATTATCAAC TACAAGACTA 
     3121 ACATTGACCG GCTGGAGGGT GACCACCAGC TGCTGCAGGA GAGCCTGGTG TTCGACAATA 
     3181 AGCACACCGT CTACAGCATG GAGCACATCC GCGTGGGCTG GGAGCAGCTG CTCACCTCCA 
     3241 TTGCCCGCAC CATCAATGAA GTGGAGAACC AGGTACTGAC CCGAGACGCC AAGGGACTGA 
     3301 GCCAGGAGCA GCTCAACGAG TTCCGAGCAT CCTTCAACCA CTTTGACAGG AAGCGGAATG 
     3361 GGATGATGGA GCCTGATGAC TTCCGAGCTT GCCTCATCTC CATGGGCTAT GACCTGGGGG 
     3421 AAGTGGAGTT TGCTCGCATC ATGACCATGG TGGACCCCAA CGCAGCTGGG GTGGTGACCT 
     3481 TCCAGGCCTT CATAGACTTC ATGACCCGAG AGACAGCCGA GACTGACACG ACTGAGCAAG 
     3541 TTGTAGCTTC CTTCAAGATC TTGGCAGGAG ACAAGAACTA CATCACCCCC GAGGAGCTGC 
     3601 GGCGCGAGCT CCCTGCCAAG CAGGCCGAGT ACTGCATCCG CCGTATGGTG CCCTACAAGG 
     3661 GATCCGGGGC CCCGGCTGGA GCCCTGGACT ACGTGGCCTT CTCCAGTGCC CTCTATGGGG 
     3721 AGAGCGACCT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3781 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3841 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3901 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3961 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4021 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4081 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4141 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4201 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4261 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4321 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4381 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4441 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4501 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4561 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4621 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4681 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4741 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4801 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4861 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4921 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4981 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5041 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5101 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5161 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5221 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5281 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5341 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5401 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5461 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5521 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5581 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5641 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5701 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5761 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5821 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5881 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5941 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6001 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6061 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6121 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6181 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6241 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6301 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6361 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6421 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6481 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6541 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6601 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6661 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6721 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6781 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6841 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6901 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6961 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7021 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7081 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7141 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7201 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7261 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7321 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7381 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7441 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7501 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7561 TAACAAAAAT TTAACGCGAA TTTT
//