LOCUS       RC211695                8844 bp    DNA     circular  SYN 
DEFINITION  GPR125 (Myc-DDK-tagged)-Human G protein-coupled receptor 125 (GPR125)
ACCESSION   NM_145290
VERSION     NM_145290.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="ADGRA3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCACCC GGACGCCGGC GGGGCCGCGC GCAGCCGCCG CTGTTGCTGC 
     1081 CGCTCTCGCT GTTAGCGCTG CTCGCGCTGC TGGGAGGCGG CGGCGGCGGC GGCGCCGCGG 
     1141 CGCTGCCCGC CGGCTGCAAG CACGATGGGC GGCCCCGAGG GGCTGGCAGG GCGGCGGGCG 
     1201 CCGCCGAGGG CAAGGTGGTG TGCAGCAGCC TGGAACTCGC GCAGGTCCTG CCCCCAGATA 
     1261 CTCTGCCCAA CCGCACGGTC ACCCTGATTC TGAGTAACAA TAAGATATCC GAGCTGAAGA 
     1321 ATGGCTCATT TTCTGGGTTA AGTCTCCTTG AAAGATTGGA CCTCCGAAAC AATCTTATTA 
     1381 GTAGTATAGA TCCAGGTGCC TTCTGGGGAC TGTCATCTCT AAAAAGATTG GATCTGACAA 
     1441 ACAATCGAAT AGGATGTCTG AATGCAGACA TATTTCGAGG ACTCACCAAT CTGGTTCGGC 
     1501 TAAACCTTTC GGGGAATTTG TTTTCTTCAT TATCTCAAGG AACTTTTGAT TATCTTGCGT 
     1561 CATTACGGTC TTTGGAATTC CAGACTGAGT ATCTTTTGTG TGACTGTAAC ATACTGTGGA 
     1621 TGCATCGCTG GGTAAAGGAG AAGAACATCA CGGTACGGGA TACCAGGTGT GTTTATCCTA 
     1681 AGTCACTGCA GGCCCAACCA GTCACAGGCG TGAAGCAGGA GCTGTTGACA TGCGACCCTC 
     1741 CGCTTGAATT GCCGTCTTTC TACATGACTC CATCTCATCG CCAAGTTGTG TTTGAAGGAG 
     1801 ACAGCCTTCC TTTCCAGTGC ATGGCTTCAT ATATTGATCA GGACATGCAA GTGTTGTGGT 
     1861 ATCAGGATGG GAGAATAGTT GAAACCGATG AATCGCAAGG TATTTTTGTT GAAAAGAACA 
     1921 TGATTCACAA CTGCTCCTTG ATTGCAAGTG CCCTAACCAT TTCTAATATT CAGGCTGGAT 
     1981 CTACTGGAAA TTGGGGCTGT CATGTCCAGA CCAAACGTGG GAATAATACG AGGACTGTGG 
     2041 ATATTGTGGT ATTAGAGAGT TCTGCACAGT ACTGTCCKCC AGAGAGGGTG GTAAACAACA 
     2101 AAGGTGACTT CAGATGGCCC AGAACATTGG CAGGCATTAC TGCATATCTG CAGTGTACGC 
     2161 GGAACACCCA TGGCAGTGGG ATATATCCCG GAAACCCACA GGATGAGAGA AAAGCTTGGC 
     2221 GCAGATGTGA TAGAGGTGGC TTTTGGGCAG ATGATGATTA TTCTCGCTGT CAGTATGCAA 
     2281 ATGATGTCAC TAGAGTTCTT TATATGTTTA ATCAGATGCC CCTCAATCTT ACCAATGCCG 
     2341 TGGCAACAGC TCGACAGTTA CTGGCTTACA CTGTGGAAGC AGCCAACTTT TCTGACAAAA 
     2401 TGGATGTTAT ATTTGTGGCA GAAATGATTG AAAAATTTGG AAGATTTACC AAGGAGGAAA 
     2461 AATCAAAAGA GCTAGGTGAC GTGATGGTTG ACATTGCAAG TAACATCATG TTGGCTGATG 
     2521 AACGTGTCCT GTGGCTGGCG CAGAGGGAAG CTAAAGCCTG CAGTAGGATT GTGCAGTGTC 
     2581 TTCAGCGCAT TGCTACCTAC CGGCTAGCCG GTGGAGCTCA CGTTTATTCA ACATATTCAC 
     2641 CCAATATTGC TCTGGAAGCT TATGTCATCA AGTCTACTGG CTTCACGGGG ATGACCTGTA 
     2701 CCGTGTTCCA GAAAGTGGCA GCCTCTGATC GTACAGGACT TTCGGATTAT GGGAGGCGGG 
     2761 ATCCAGAGGG AAACCTGGAT AAGCAGCTGA GCTTTAAGTG CAATGTTTCA AATACATTTT 
     2821 CGAGTCTGGC ACTAAAGAAT ACTATTGTGG AGGCTTCTAT TCAGCTTCCT CCTTCCCTTT 
     2881 TCTCACCAAA GCAAAAAAGA GAACTCAGAC CAACTGATGA CTCTCTTTAC AAGCTTCAAC 
     2941 TCATTGCATT CCGCAATGGA AAGCTTTTTC CAGCCACTGG AAATTCAACA AATTTGGCTG 
     3001 ATGATGGAAA ACGACGTACT GTGGTTACCC CTGTGATTCT CACCAAAATA GATGGTGTGA 
     3061 ATGTAGATAC CCACCACATC CCTGTTAATG TGACACTGCG TCGAATTGCA CATGGAGCAG 
     3121 ATGCTGTTGC AGCCCGGTGG GATTTCGATT TGCTGAACGG ACAAGGAGGC TGGAAGTCAG 
     3181 ATGGGTGCCA TATACTCTAT TCAGATGAAA ATATCACTAC GATTCAGTGC TACTCCCTTA 
     3241 GTAACTATGC AGTTTTAATG GATTTGACGG GATCTGAACT ATACACCCAG GCGGCCAGCC 
     3301 TCCTGCATCC TGTGGTTTAT ACTACCGCTA TCATTCTCCT CTTATGTCTC TTAGCCGTCA 
     3361 TTGTCAGTTA CATATACCAT CACAGTTTGA TTAGAATCAG CCTCAAGAGC TGGCACATGC 
     3421 TTGTGAACTT GTGCTTTCAT ATTTTCCTAA CCTGTGTGGT CTTTGTGGGA GGAATAACCC 
     3481 AGACTAGGAA TGCCAGCATC TGCCAAGCAG TTGGGATAAT TCTTCACTAT TCCACCCTTG 
     3541 CCACAGTACT ATGGGTAGGA GTGACAGCTC GAAATATCTA CAAACAAGTC ACTAAAAAAG 
     3601 CTAAAAGATG CCAGGATCCT GATGAACCAC CACCTCCACC AAGACCAATG CTCAGATTTT 
     3661 ACCTGATTGG TGGTGGTATC CCCATCATTG TTTGCGGCAT AACTGCAGCA GCGAACATTA 
     3721 AGAATTACGG CAGTCGGCCA AACGCACCCT ATTGCTGGAT GGCATGGGAA CCCTCCTTGG 
     3781 GAGCCTTCTA TGGGCCAGCC AGCTTCATCA CTTTTGTAAA CTGCATGTAC TTTCTGAGCA 
     3841 TATTTATTCA GTTGAAAAGA CACCCTGAGC GCAAATATGA GCTTAAGGAG CCCACGGAGG 
     3901 AGCAACAGAG ATTGGCAGCC AATGAAAATG GCGAAATAAA TCATCAGGAT TCAATGTCTT 
     3961 TGTCTCTGAT TTCTACATCA GCCTTGGAAA ATGAGCACAC TTTTCATTCT CAGCTCTTGG 
     4021 GGGCCAGCCT TACTTTGCTC TTATATGTTG CACTGTGGAT GTTTGGGGCT TTGGCTGTTT 
     4081 CTTTGTATTA CCCTTTGGAC TTGGTTTTTA GCTTCGTTTT TGGAGCCACA AGTTTAAGCT 
     4141 TCAGTGCGTT CTTCGTGGTC CACCATTGTG TTAATAGGGA GGATGTTAGA CTTGCGTGGA 
     4201 TCATGACTTG CTGCCCAGGA CGGAGCTCGT ATTCAGTGCA AGTCAACGTC CAGCCCCCCA 
     4261 ACTCTAATGG GACGAATGGA GAGGCACCCA AATGCCCCAA TAGCAGTGCG GAGTCTTCAT 
     4321 GCACAAACAA AAGTGCTTCA AGCTTCAAAA ATTCCTCCCA GGGCTGCAAA TTAACAAACT 
     4381 TGCAGGCGGC TGCAGCTCAG TGCCATGCCA ATTCTTTACC TTTGAACTCC ACCCCTCAGC 
     4441 TTGATAATAG TCTGACAGAA CATTCAATGG ACAATGATAT TAAAATGCAC GTGGCGCCTT 
     4501 TAGAAGTTCA GTTTCGAACA AATGTGCACT CAAGCCGCCA CCATAAAAAC AGAAGTAAAG 
     4561 GACACCGGGC AAGCCGACTC ACAGTCCTGA GAGAATATGC CTACGATGTC CCAACGAGCG 
     4621 TGGAAGGAAG CGTGCAGAAC GGCTTACCTA AAAGCCGGCT GGGCAATAAC GAAGGACACT 
     4681 CGAGGAGCCG AAGAGCTTAT TTAGCCTACA GAGAGAGACA GTACAACCCA CCCCAGCAAG 
     4741 ACAGCAGCGA TGCTTGTAGC ACACTTCCCA AAAGTAGCAG AAATTTTGAA AAGCCAGTTT 
     4801 CAACCACTAG TAAAAAAGAT GCGTTAAGGA AGCCAGCTGT GGTTGAACTT GAAAATCAGC 
     4861 AAAAATCTTA TGGCCTCAAC TTGGCCATTC AGAATGGACC AATTAAAAGC AATGGGCAGG 
     4921 AGGGACCCTT GCTCGGTACC GATAGCACTG GCAATGTTAG GACTGGATTA TGGAAACACG 
     4981 AAACTACTGT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//