LOCUS       RC211676                7617 bp    DNA     circular  SYN 
DEFINITION  PRKD1 (Myc-DDK-tagged)-Human protein kinase D1 (PRKD1)
ACCESSION   NM_002742
VERSION     NM_002742.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..3865 
                     /label="Myc-DDK"  
     misc_feature    3895..4480 
                     /label="PolyA signal"  
     misc_feature    3952..3971 
                     /label="Restriction Enzyme"  
     misc_feature    4756..5428 
                     /label="ColE1"  
     misc_feature    5858..6652 
                     /label="Kan/Neo"  
     misc_feature    6687..6989 
                     /label="SV40 ori"  
     misc_feature    7075..7552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3756..3762 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3756 
                     /label="PRKD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGCCCCT CCGGTCCTGC GGCCGCCCAG TCCGCTGCTG CCCGTGGCGG 
     1081 CGGCAGCTGC CGCAGCGGCC GCCGCACTGG TCCCAGGGTC CGGGCCCGGG CCCGCGCCGT 
     1141 TCTTGGCTCC TGTCGCGGCC CCGGTCGGGG GCATCTCGTT CCATCTGCAG ATCGGCCTGA 
     1201 GCCGTGAGCC GGTGCTGCTG CTGCAGGACT CGTCCGGGGA CTACAGCCTG GCGCACGTCC 
     1261 GCGAGATGGC TTGCTCCATT GTCGACCAGA AGTTCCCTGA ATGTGGTTTC TACGGAATGT 
     1321 ATGATAAGAT CCTGCTTTTT CGCCATGACC CTACCTCTGA AAACATCCTT CAGCTGGTGA 
     1381 AAGCGGCCAG TGATATCCAG GAAGGCGATC TTATTGAAGT GGTCTTGTCA GCTTCCGCCA 
     1441 CCTTTGAAGA CTTTCAGATT CGTCCCCACG CTCTCTTTGT TCATTCATAC AGAGCTCCAG 
     1501 CTTTCTGTGA TCACTGTGGA GAAATGCTGT GGGGGCTGGT ACGTCAAGGT CTTAAATGTG 
     1561 AAGGGTGTGG TCTGAATTAC CATAAGAGAT GTGCATTTAA AATACCCAAC AATTGCAGCG 
     1621 GTGTGAGGCG GAGAAGGCTC TCAAACGTTT CCCTCACTGG GGTCAGCACC ATCCGCACAT 
     1681 CATCTGCTGA ACTCTCTACA AGTGCCCCTG ATGAGCCCCT TCTGCAAAAA TCACCATCAG 
     1741 AGTCGTTTAT TGGTCGAGAG AAGAGGTCAA ATTCTCAATC ATACATTGGA CGACCAATTC 
     1801 ACCTTGACAA GATTTTGATG TCTAAAGTTA AAGTGCCGCA CACATTTGTC ATCCACTCCT 
     1861 ACACCCGGCC CACAGTGTGC CAGTACTGCA AGAAGCTTCT GAAGGGGCTT TTCAGGCAGG 
     1921 GCTTGCAGTG CAAAGATTGC AGATTCAACT GCCATAAACG TTGTGCACCG AAAGTACCAA 
     1981 ACAACTGCCT TGGCGAAGTG ACCATTAATG GAGATTTGCT TAGCCCTGGG GCAGAGTCTG 
     2041 ATGTGGTCAT GGAAGAAGGG AGTGATGACA ATGATAGTGA AAGGAACAGT GGGCTCATGG 
     2101 ATGATATGGA AGAAGCAATG GTCCAAGATG CAGAGATGGC AATGGCAGAG TGCCAGAACG 
     2161 ACAGTGGCGA GATGCAAGAT CCAGACCCAG ACCACGAGGA CGCCAACAGA ACCATCAGTC 
     2221 CATCAACAAG CAACAATATC CCACTCATGA GGGTAGTGCA GTCTGTCAAA CACACGAAGA 
     2281 GGAAAAGCAG CACAGTCATG AAAGAAGGAT GGATGGTCCA CTACACCAGC AAGGACACGC 
     2341 TGCGGAAACG GCACTATTGG AGATTGGATA GCAAATGTAT TACCCTCTTT CAGAATGACA 
     2401 CAGGAAGCAG GTACTACAAG GAAATTCCTT TATCTGAAAT TTTGTCTCTG GAACCAGTAA 
     2461 AAACTTCAGC TTTAATTCCT AATGGGGCCA ATCCTCATTG TTTCGAAATC ACTACGGCAA 
     2521 ATGTAGTGTA TTATGTGGGA GAAAATGTGG TCAATCCTTC CAGCCCATCA CCAAATAACA 
     2581 GTGTTCTCAC CAGTGGCGTT GGTGCAGATG TGGCCAGGAT GTGGGAGATA GCCATCCAGC 
     2641 ATGCCCTTAT GCCCGTCATT CCCAAGGGCT CCTCCGTGGG TACAGGAACC AACTTGCACA 
     2701 GAGATATCTC TGTGAGTATT TCAGTATCAA ATTGCCAGAT TCAAGAAAAT GTGGACATCA 
     2761 GCACAGTATA TCAGATTTTT CCTGATGAAG TACTGGGTTC TGGACAGTTT GGAATTGTTT 
     2821 ATGGAGGAAA ACATCGTAAA ACAGGAAGAG ATGTAGCTAT TAAAATCATT GACAAATTAC 
     2881 GATTTCCAAC AAAACAAGAA AGCCAGCTTC GTAATGAGGT TGCAATTCTA CAGAACCTTC 
     2941 ATCACCCTGG TGTTGTAAAT TTGGAGTGTA TGTTTGAGAC GCCTGAAAGA GTGTTTGTTG 
     3001 TTATGGAAAA ACTCCATGGA GACATGCTGG AAATGATCTT GTCAAGTGAA AAGGGCAGGT 
     3061 TGCCAGAGCA CATAACGAAG TTTTTAATTA CTCAGATACT CGTGGCTTTG CGGCACCTTC 
     3121 ATTTTAAAAA TATCGTTCAC TGTGACCTCA AACCAGAAAA TGTGTTGCTA GCCTCAGCTG 
     3181 ATCCTTTTCC TCAGGTGAAA CTTTGTGATT TTGGTTTTGC CCGGATCATC GGAGAGAAGT 
     3241 CTTTCCGGAG GTCAGTGGTG GGTACCCCCG CTTACCTGGC TCCTGAGGTC CTAAGGAACA 
     3301 AGGGCTACAA TCGCTCTCTA GACATGTGGT CTGTTGGGGT CATCATCTAT GTAAGCCTAA 
     3361 GCGGCACATT CCCATTTAAT GAAGATGAAG ACATACACGA CCAAATTCAG AATGCAGCTT 
     3421 TCATGTATCC ACCAAATCCC TGGAAGGAAA TATCTCATGA AGCCATTGAT CTTATCAACA 
     3481 ATTTGCTGCA AGTAAAAATG AGAAAGCGCT ACAGTGTGGA TAAGACCTTG AGCCACCCTT 
     3541 GGCTACAGGA CTATCAGACC TGGTTAGATT TGCGAGAGCT GGAATGCAAA ATCGGGGAGC 
     3601 GCTACATCAC CCATGAAAGT GATGACCTGA GGTGGGAGAA GTATGCAGGC GAGCAGGGGC 
     3661 TGCAGTACCC CACACACCTG ATCAATCCAA GTGCTAGCCA CAGTGACACT CCTGAGACTG 
     3721 AAGAAACAGA AATGAAAGCC CTCGGTGAGC GTGTCAGCAT CCTCACGCGT ACGCGGCCGC 
     3781 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3841 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3901 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3961 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4021 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4081 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4141 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4201 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4261 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4321 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4381 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4441 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4501 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5401 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5461 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5521 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5581 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5641 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5701 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5761 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5821 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5881 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5941 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6001 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6061 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6121 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6181 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6241 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6301 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6361 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6421 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6481 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6541 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6601 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6661 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6721 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6781 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6841 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7021 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7081 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7141 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7201 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7261 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7321 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7381 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7441 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7501 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7561 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//