LOCUS       RC211602                8496 bp    DNA     circular  SYN 
DEFINITION  ADAMTS3 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3)
ACCESSION   NM_014243
VERSION     NM_014243.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="ADAMTS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTCTCCTG TCACTTTGGT TGATAGCAGC CGCTCTGGTA GAGGTTAGGA 
     1081 CTTCAGCTGA TGGACAAGCT GGTAATGAAG AAATGGTGCA AATAGATTTA CCAATAAAGA 
     1141 GATATAGAGA GTATGAGCTG GTGACTCCAG TCAGCACAAA TCTAGAAGGA CGCTATCTCT 
     1201 CCCATACTCT TTCTGCGAGT CACAAAAAGA GGTCAGCGAG GGACGTGTCT TCCAACCCTG 
     1261 AGCAGTTGTT CTTTAACATC ACGGCATTTG GAAAAGATTT TCATCTGCGA CTAAAGCCCA 
     1321 ACACTCAACT AGTAGCTCCT GGGGCTGTTG TGGAGTGGCA TGAGACATCT CTGGTGCCTG 
     1381 GGAATATAAC CGATCCCATT AACAACCATC AACCAGGAAG TGCTACGTAT AGAATCCGGA 
     1441 AAACAGAGCC TTTGCAGACT AACTGTGCTT ATGTTGGTGA CATCGTGGAC ATTCCAGGAA 
     1501 CCTCTGTTGC CATCAGCAAC TGTGATGGTC TGGCTGGAAT GATAAAAAGT GATAATGAAG 
     1561 AGTATTTCAT TGAACCCTTG GAAAGAGGTA AACAGATGGA GGAAGAAAAA GGAAGGATTC 
     1621 ATGTTGTCTA CAAGAGATCA GCTGTAGAAC AGGCTCCCAT AGACATGTCC AAAGACTTCC 
     1681 ACTACAGAGA GTCGGACCTG GAAGGCCTTG ATGATCTAGG TACTGTTTAT GGCAACATCC 
     1741 ACCAGCAGCT GAATGAAACA ATGAGACGCC GCAGACACGC GGGAGAAAAC GATTACAATA 
     1801 TCGAGGTACT GCTGGGAGTG GATGACTCTG TGGTCCGTTT CCATGGCAAA GAGCACGTCC 
     1861 AAAACTACCT CCTGACCCTA ATGAACATTG TGAATGAAAT TTACCATGAT GAGTCCCTCG 
     1921 GAGTGCATAT AAATGTGGTC CTGGTGCGCA TGATAATGCT GGGATATGCA AAGTCCATCA 
     1981 GCCTCATAGA AAGGGGAAAC CCATCCAGAA GCTTGGAGAA TGTGTGTCGC TGGGCGTCCC 
     2041 AACAGCAAAG ATCTGATCTC AACCACTCTG AACACCATGA CCATGCAATT TTTTTAACCA 
     2101 GGCAAGACTT TGGACCTGCT GGAATGCAAG GATATGCTCC AGTCACCGGC ATGTGTCATC 
     2161 CAGTGAGAAG TTGTACCCTG AATCATGAGG ATGGTTTTTC ATCTGCTTTT GTAGTAGCCC 
     2221 ATGAAACGGG CCATGTGTTG GGAATGGAGC ATGATGGACA AGGCAACAGG TGTGGTGATG 
     2281 AGACTGCTAT GGGAAGTGTC ATGGCTCCCT TGGTACAAGC AGCATTCCAT CGTTACCACT 
     2341 GGTCCCGATG CAGTGGTCAA GAACTGAAAA GATATATCCA TTCCTATGAC TGTCTCCTTG 
     2401 ATGACCCTTT TGATCATGAT TGGCCTAAAC TCCCAGAACT TCCTGGAATC AATTATTCTA 
     2461 TGGATGAGCA ATGTCGTTTT GATTTTGGTG TTGGCTATAA AATGTGCACC GCGTTCCGAA 
     2521 CCTTTGACCC ATGTAAACAG CTGTGGTGTA GCCATCCTGA TAATCCCTAC TTTTGTAAGA 
     2581 CTAAAAAGGG ACCTCCACTT GATGGGACTG AATGTGCTGC TGGAAAATGG TGCTATAAGG 
     2641 GTCATTGCAT GTGGAAGAAT GCTAATCAGC AAAAACAAGA TGGCAATTGG GGGTCATGGA 
     2701 CTAAATTTGG CTCCTGTTCT CGGACATGTG GAACTGGTGT TCGTTTCAGA ACACGCCAGT 
     2761 GCAATAATCC CATGCCCATC AATGGTGGTC AGGATTGTCC TGGTGTTAAT TTTGAGTACC 
     2821 AGCTTTGTAA CACAGAAGAA TGCCAAAAAC ACTTTGAGGA CTTCAGAGCA CAGCAGTGTC 
     2881 AGCAGCGAAA CTCCCACTTT GAATACCAGA ATACCAAACA CCACTGGTTG CCATATGAAC 
     2941 ATCCTGACCC CAAGAAAAGA TGCCACCTTT ACTGTCAGTC CAAGGAGACT GGAGATGTTG 
     3001 CTTACATGAA ACAACTGGTG CATGATGGAA CGCACTGTTC TTACAAAGAT CCATATAGCA 
     3061 TATGTGTGCG AGGAGAGTGT GTGAAAGTGG GCTGTGATAA AGAAATTGGT TCTAATAAGG 
     3121 TTGAGGATAA GTGTGGTGTC TGTGGAGGAG ATAATTCCCA CTGCCGAACC GTGAAGGGGA 
     3181 CATTTACCAG AACTCCCAGG AAGCTTGGGT ACCTTAAGAT GTTTGATATA CCCCCTGGGG 
     3241 CTAGACATGT GTTAATCCAA GAAGACGAGG CTTCTCCTCA TATTCTTGCT ATTAAGAACC 
     3301 AGGCTACAGG CCATTATATT TTAAATGGCA AAGGGGAGGA AGCCAAGTCG CGGACCTTCA 
     3361 TAGATCTTGG TGTGGAGTGG GATTATAACA TTGAAGATGA CATTGAAAGT CTTCACACCG 
     3421 ATGGACCTTT ACATGATCCT GTTATTGTTT TGATTATACC TCAAGAAAAT GATACCCGCT 
     3481 CTAGCCTGAC ATATAAGTAC ATCATCCATG AAGACTCTGT ACCTACAATC AACAGCAACA 
     3541 ATGTCATCCA GGAAGAATTA GATACTTTTG AGTGGGCTTT GAAGAGCTGG TCTCAGTGTT 
     3601 CCAAACCCTG TGGTGGAGGT TTCCAGTACA CTAAATATGG ATGCCGTAGG AAAAGTGATA 
     3661 ATAAAATGGT CCATCGCAGC TTCTGTGAGG CCAACAAAAA GCCGAAACCT ATTAGACGAA 
     3721 TGTGCAATAT TCAAGAGTGT ACACATCCAC TCTGGGTAGC AGAAGAATGG GAACACTGCA 
     3781 CCAAAACCTG TGGAAGTTCT GGCTATCAGC TTCGCACTGT ACGCTGCCTT CAGCCACTCC 
     3841 TTGATGGCAC CAACCGCTCT GTGCACAGCA AATACTGCAT GGGTGACCGT CCCGAGAGCC 
     3901 GCCGGCCCTG TAACAGAGTG CCCTGCCCTG CACAGTGGAA AACAGGACCC TGGAGTGAGT 
     3961 GTTCAGTGAC CTGCGGTGAA GGAACGGAGG TGAGGCAGGT CCTCTGCAGG GCTGGGGACC 
     4021 ACTGTGATGG TGAAAAGCCT GAGTCGGTCA GAGCCTGTCA ACTGCCTCCT TGTAATGATG 
     4081 AACCATGTTT GGGAGACAAG TCCATATTCT GTCAAATGGA AGTGTTGGCA CGATACTGCT 
     4141 CCATACCAGG TTATAACAAG TTATGTTGTG AGTCCTGCAG CAAGCGCAGT AGCACCCTGC 
     4201 CACCACCATA CCTTCTAGAA GCTGCTGAAA CTCATGATGA TGTCATCTCT AACCCTAGTG 
     4261 ACCTCCCTAG ATCTCTAGTG ATGCCTACAT CTTTGGTTCC TTATCATTCA GAGACCCCTG 
     4321 CAAAGAAGAT GTCTTTGAGT AGCATCTCTT CAGTGGGAGG TCCAAATGCA TATGCTGCTT 
     4381 TCAGGCCAAA CAGTAAACCT GATGGTGCTA ATTTACGCCA GAGGAGTGCT CAGCAAGCAG 
     4441 GAAGTAAGAC TGTGAGACTG GTCACCGTAC CATCCTCCCC ACCCACCAAG AGGGTCCACC 
     4501 TCAGTTCAGC TTCACAAATG GCTGCTGCTT CCTTCTTTGC AGCCAGTGAT TCAATAGGTG 
     4561 CTTCTTCTCA GGCAAGAACC TCAAAGAAAG ATGGAAAGAT CATTGACAAC AGACGTCCGA 
     4621 CAAGATCATC CACCTTAGAA AGAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//