LOCUS       RC211597                8907 bp    DNA     circular  SYN 
DEFINITION  ZNF335 (Myc-DDK-tagged)-Human zinc finger protein 335 (ZNF335)
ACCESSION   NM_022095
VERSION     NM_022095.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5064..5155 
                     /label="Myc-DDK"  
     misc_feature    5185..5770 
                     /label="PolyA signal"  
     misc_feature    5242..5261 
                     /label="Restriction Enzyme"  
     misc_feature    6046..6718 
                     /label="ColE1"  
     misc_feature    7148..7942 
                     /label="Kan/Neo"  
     misc_feature    7977..8279 
                     /label="SV40 ori"  
     misc_feature    8365..8842 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5046..5052 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5046 
                     /label="ZNF335"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGAAC GAGGTGGAGA GCAGCAGCGA CGCGGCCCCT GGGCCTGGCC 
     1081 GGCCCGAGGA GCCCTCTGAG AGCGGCCTGG GTGTGGGCAC CTCAGAAGCC GTGTCCGCCG 
     1141 ACAGCAGCGA CGCCGCGGCC GCCCCGGGGC AGGCAGAGGC CGATGACTCT GGCGTGGGGC 
     1201 AAAGCTCGGA CCGCGGCAGC CGTTCTCAGG AGGAGGTATC TGAGAGCAGC TCGAGCGCAG 
     1261 ACCCCCTGCC TAATAGCTAC CTCCCTGATT CATCGTCTGT GTCTCATGGG CCAGTGGCAG 
     1321 GGGTGACAGG CGGTCCCCCA GCACTTGTGC ACTCTAGTGC ACTCCCAGAC CCCAACATGC 
     1381 TGGTGTCCGA CTGCACAGCT TCCTCCTCGG ACCTGGGCTC GGCCATCGAC AAGATCATCG 
     1441 AGTCCACCAT CGGGCCCGAC CTCATCCAGA ACTGCATCAC TGTGACCAGT GCTGAGGATG 
     1501 GCGGGGCCGA GACCACACGG TACCTGATCC TACAGGGCCC AGATGATGGA GCCCCCATGA 
     1561 CATCACCAAT GTCCAGTTCC ACCTTGGCCC ACAGCCTAGC AGCCATTGAG GCCCTGGCAG 
     1621 ATGGCCCCAC ATCCACATCC ACATGCCTGG AGGCACAGGG TGGGCCCAGC TCCCCGGTGC 
     1681 AGCTGCCCCC AGCCTCCGGT GCCGAAGAGC CGGACCTGCA GAGCCTGGAG GCCATGATGG 
     1741 AGGTGGTGGT GGTGCAGCAG TTCAAATGCA AGATGTGCCA GTACCGGAGC AGCACCAAGG 
     1801 CCACACTGCT GCGCCACATG CGGGAACGCC ACTTCCGTCC AGTAGCAGCA GCCGCAGCAG 
     1861 CAGCTGGTAA AAAAGGACGT CTACGGAAGT GGAGCACCTC CACCAAGAGC CAAGAGGAAG 
     1921 AGGGACCAGA GGAGGAGGAC GATGATGACA TTGTAGACGC TGGAGCCATT GATGACCTGG 
     1981 AGGAGGATAG CGACTATAAT CCAGCTGAGG ATGAGCCCCG AGGCCGGCAG CTTCGGCTCC 
     2041 AGCGCCCCAC CCCCAGTACC CCAAGGCCCC GAAGGAGACC TGGCCGGCCC CGGAAGCTGC 
     2101 CCCGCCTGGA GATCTCAGAC CTCCCAGATG GTGTGGAAGG AGAGCCTCTA GTGAGTTCCC 
     2161 AGAGTGGACA GAGCCCTCCA GAGCCACAGG ATCCCGAGGC TCCCAGCTCC TCAGGCCCAG 
     2221 GACACCTGGT GGCCATGGGC AAGGTGAGCA GGACCCCTGT GGAAGCTGGT GTGAGCCAGT 
     2281 CAGATGCAGA GAACGCAGCC CCCTCCTGCC CGGATGAGCA TGACACTCTG CCCCGGCGCC 
     2341 GAGGTCGACC TTCCAGGCGC TTCCTAGGCA AGAAATACCG CAAGTACTAT TACAAGTCGC 
     2401 CCAAACCACT TTTGAGGCCC TTCCTGTGCC GCATCTGTGG TTCTCGCTTT CTGTCCCACG 
     2461 AGGACCTGCG CTTCCACGTC AACTCCCATG AGGCTGGCGA TCCCCAGCTC TTCAAGTGCC 
     2521 TGCAGTGCAG CTATCGTTCC CGCCGCTGGT CCTCGCTCAA GGAGCACATG TTCAACCACG 
     2581 TGGGCAGCAA GCCCTACAAG TGTGACGAGT GCAGCTACAC CAGTGTCTAC CGGAAGGACG 
     2641 TCATTCGGCA CGCCGCTGTG CACAGCCGGG ACCGGAAGAA GAGGCCAGAT CCGACTCCAA 
     2701 AGCTGAGCTC TTTCCCCTGC CCTGTGTGTG GCCGTGTGTA CCCCATGCAG AAAAGACTCA 
     2761 CGCAGCACAT GAAGACGCAC AGCACTGAGA AGCCCCACAT GTGTGACAAG TGTGGAAAGT 
     2821 CCTTTAAGAA GCGCTACACC TTCAAAATGC ACCTGCTCAC GCACATCCAG GCTGTTGCCA 
     2881 ACCGCAGGTT CAAGTGTGAG TTCTGTGAGT TTGTTTGTGA AGACAAGAAG GCACTGCTGA 
     2941 ACCACCAGTT GTCCCACGTC AGTGACAAGC CCTTCAAATG CAGCTTTTGT CCCTACCGCA 
     3001 CCTTCCGAGA GGACTTCTTG CTGTCCCATG TGGCTGTCAA GCACACAGGG GCCAAGCCCT 
     3061 TCGCCTGTGA GTACTGCCAC TTCAGCACAC GGCACAAGAA GAACCTGCGC CTGCACGTAC 
     3121 GGTGCCGACA CGCAAGCAGC TTCGAGGAAT GGGGGAGGCG CCACCCTGAG GAGCCCCCCT 
     3181 CCCGCCGTCG CCCCTTCTTC TCTCTGCAGC AGATTGAGGA GCTGAAGCAG CAGCACAGTG 
     3241 CGGCCCCTGG ACCACCTCCC AGTTCCCCAG GACCTCCTGA GATACCCCCA GAGGCGACAA 
     3301 CTTTCCAGTC ATCTGAGGCT CCCTCATTGC TCTGTTCTGA CACCCTGGGC GGCGCCACCA 
     3361 TCATCTACCA GCAAGGAGCT GAGGAGTCGA CAGCGATGGC CACGCAGACA GCCTTGGATC 
     3421 TTCTGCTGAA CATGAGTGCT CAGCGGGAAC TGGGGGGCAC AGCCCTGCAG GTGGCTGTGG 
     3481 TGAAGTCGGA AGATGTGGAA GCAGGGTTAG CATCCCCTGG TGGGCAGCCC TCCCCTGAAG 
     3541 GTGCCACTCC ACAGGTGGTC ACCCTCCACG TGGCAGAGCC AGGGGGCGGT GCAGCAGCCG 
     3601 AGAGCCAGCT AGGCCCTCCT GACCTACCGC AGATCACCCT GGCACCTGGT CCATTTGGTG 
     3661 GGACTGGCTA CAGTGTCATC ACAGCACCCC CTATGGAGGA GGGAACATCA GCTCCTGGCA 
     3721 CACCTTACAG CGAGGAGCCC GCAGGAGAGG CAGCCCAGGC TGTGGTTGTG AGTGACACCC 
     3781 TAAAAGAAGC TGGCACCCAC TACATCATGG CTACTGATGG TACCCAGTTG CACCACATTG 
     3841 AGCTCACCGC AGATGGCTCC ATCTCCTTCC CAAGTCCAGA TGCTCTGGCC TCTGGTGCCA 
     3901 AATGGCCCCT GCTGCAGTGT GGGGGACTGC CCAGAGACGG CCCTGAGCCC CCATCTCCAG 
     3961 CCAAGACCCA CTGCGTAGGG GACTCCCAGA GCTCTGCCTC CTCACCTCCT GCAACCAGCA 
     4021 AAGCCCTGGG CCTGGCAGTG CCCCCGTCAC CGCCATCTGC AGCCACTGCT GCATCAAAGA 
     4081 AGTTTTCCTG CAAGATCTGT GCCGAGGCCT TCCCTGGCCG AGCTGAGATG GAGAGTCACA 
     4141 AGCGGGCCCA CGCTGGGCCT GGTGCCTTCA AGTGCCCCGA CTGCCCCTTC AGTGCCCGCC 
     4201 AGTGGCCCGA GGTCCGGGCG CACATGGCAC AGCACTCAAG CCTACGGCCC CACCAGTGTA 
     4261 GCCAGTGCAG CTTTGCCTCC AAGAACAAGA AGGACCTGCG TCGGCACATG CTGACTCACA 
     4321 CAAAGGAGAA GCCTTTTGCA TGCCACCTCT GCGGGCAGCG TTTCAACCGT AACGGGCACC 
     4381 TCAAGTTCCA CATCCAGCGG CTGCACAGTC CTGATGGGAG GAAGTCAGGA ACCCCTACAG 
     4441 CCCGGGCCCC TACCCAGACC CCAACCCAGA CCATCATCCT GAACAGTGAT GACGAAACAC 
     4501 TGGCCACCCT GCACACTGCA CTCCAGTCCA GTCACGGGGT CCTGGGCCCA GAGCGGCTAC 
     4561 AGCAGGCACT GAGCCAGGAA CACATCATCG TTGCCCAGGA ACAGACAGTG ACCAATCAGG 
     4621 AGGAAGCCGC CTACATCCAA GAGATCACCA CGGCAGATGG CCAGACCGTA CAGCACCTGG 
     4681 TGACCTCCGA CAACCAGGTG CAGTATATCA TCTCCCAGGA TGGTGTCCAG CACCTGCTCC 
     4741 CCCAGGAATA TGTTGTGGTC CCTGAAGGCC ATCACATCCA GGTACAGGAG GGCCAGATCA 
     4801 CACACATCCA GTATGAACAA GGAGCCCCGT TCCTTCAGGA GTCCCAGATC CAGTATGTGC 
     4861 CTGTGTCCCC AGGCCAGCAG CTTGTCACAC AGGCTCAACT TGAGGCTGCA GCACACTCAG 
     4921 CTGTCACAGC AGTGGCTGAT GCTGCCATGG CCCAAGCCCA GGGCCTGTTT GGTACAGACG 
     4981 AGACAGTGCC CGAACACATT CAACAGCTGC AGCACCAGGG CATCGAGTAC GACGTCATCA 
     5041 CCCTGGCCGA TGACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5101 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5161 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5221 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5281 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5341 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5401 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5461 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5521 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5581 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5641 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5701 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5761 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5821 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5881 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5941 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6001 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6061 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6121 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6181 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6241 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6301 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6361 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6421 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6481 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6541 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6601 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6661 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6721 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6781 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6841 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6901 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6961 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7021 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7081 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7141 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7201 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7261 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7321 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7381 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7441 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7501 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7561 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7621 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7681 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7741 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7801 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7861 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7921 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7981 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8041 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8101 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8161 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8221 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8281 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8341 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8401 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8461 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8521 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8581 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8641 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8701 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8761 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8821 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8881 ATTTAACAAA AATTTAACGC GAATTTT
//