LOCUS       RC211584                7044 bp    DNA     circular  SYN 
DEFINITION  EPB42 (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.2 (EPB42), transcript variant 1
ACCESSION   NM_000119
VERSION     NM_000119.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3201..3292 
                     /label="Myc-DDK"  
     misc_feature    3322..3907 
                     /label="PolyA signal"  
     misc_feature    3379..3398 
                     /label="Restriction Enzyme"  
     misc_feature    4183..4855 
                     /label="ColE1"  
     misc_feature    5285..6079 
                     /label="Kan/Neo"  
     misc_feature    6114..6416 
                     /label="SV40 ori"  
     misc_feature    6502..6979 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3183..3189 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3183 
                     /label="EPB42"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGACAGGGT GAGCCAAGCC AGCGCTCGAC AGGGCTTGCT GGACTGTATG 
     1081 CAGCCCCCGC AGCATCACCT GTTTTCATTA AAGGAAGTGG GATGGATGCC CTGGGTATCA 
     1141 AGAGCTGTGA CTTTCAGGCA GCAAGAAACA ATGAGGAGCA CCACACCAAG GCCCTCAGCT 
     1201 CCCGGCGCCT CTTTGTGAGG AGGGGGCAGC CCTTCACCAT CATCCTGTAC TTCCGCGCTC 
     1261 CAGTCCGTGC ATTTCTGCCT GCCCTGAAGA AGGTGGCCCT CACTGCACAA ACTGGAGAGC 
     1321 AGCCTTCCAA GATCAACAGG ACCCAAGCCA CATTCCCAAT TTCCAGTCTG GGGGACCGAA 
     1381 AGTGGTGGAG TGCAGTGGTG GAGGAGAGAG ATGCCCAGTC CTGGACCATC TCTGTGACCA 
     1441 CACCTGCGGA CGCTGTCATT GGCCACTACT CGCTTCTGCT GCAGGTCTCA GGCAGGAAGC 
     1501 AACTCCTCTT GGGTCAGTTC ACACTGCTTT TTAACCCCTG GAATAGAGAG GATGCTGTGT 
     1561 TCCTGAAGAA TGAGGCTCAG CGCATGGAGT ACTTGTTGAA CCAGAATGGT CTCATCTACC 
     1621 TGGGTACAGC TGACTGCATC CAGGCAGAGT CCTGGGACTT TGGCCAGTTC GAGGGGGATG 
     1681 TCATTGACCT CAGCCTGCGC TTGCTGAGCA AGGACAAGCA GGTAGAGAAG TGGAGCCAGC 
     1741 CGGTGCACGT GGCCCGTGTG TTGGGTGCCT TGCTGCATTT TCTCAAGGAG CAGAGGGTCC 
     1801 TGCCCACCCC GCAGACCCAG GCCACCCAGG AAGGGGCCTT GCTGAACAAG CGCCGGGGCA 
     1861 GCGTGCCCAT CCTGCGGCAG TGGCTCACCG GCCGAGGCCG ACCTGTGTAT GATGGCCAGG 
     1921 CCTGGGTGTT GGCTGCTGTT GCTTGCACAG TGCTGCGATG CCTGGGAATC CCTGCCCGCG 
     1981 TGGTGACCAC GTTTGCCTCA GCACAGGGCA CCGGTGGGCG TCTTCTCATA GATGAATACT 
     2041 ATAATGAGGA GGGACTTCAG AACGGAGAAG GCCAGAGAGG CAGAATCTGG ATCTTCCAGA 
     2101 CTTCCACAGA GTGCTGGATG ACGCGGCCTG CCTTGCCCCA GGGTTATGAT GGATGGCAGA 
     2161 TTCTGCACCC AAGTGCTCCT AATGGAGGTG GAGTCCTGGG GTCCTGTGAT CTGGTGCCGG 
     2221 TCAGAGCAGT CAAGGAGGGG ACGCTGGGGC TGACCCCAGC AGTGTCAGAC CTTTTTGCTG 
     2281 CCATAAATGC CTCATGTGTG GTCTGGAAGT GCTGTGAGGA TGGGACACTG GAGTTGACTG 
     2341 ACTCCAACAC AAAGTATGTT GGCAACAACA TCAGCACCAA GGGTGTGGGC AGTGACCGCT 
     2401 GCGAGGACAT CACTCAGAAC TACAAGTATC CTGAAGGGTC TCTTCAGGAA AAAGAGGTGC 
     2461 TGGAGAGAGT CGAGAAAGAG AAAATGGAAC GTGAGAAAGA CAACGGCATC CGTCCTCCCA 
     2521 GTCTCGAGAC TGCCAGTCCT CTGTACCTGC TCTTGAAAGC ACCCAGCTCC CTACCCCTGA 
     2581 GAGGGGATGC CCAGATCTCA GTGACGCTGG TTAATCACAG TGAGCAGGAG AAGGCAGTGC 
     2641 AGCTGGCAAT TGGGGTCCAG GCTGTACACT ACAACGGTGT CCTTGCTGCC AAGCTCTGGA 
     2701 GGAAGAAGCT GCACCTCACG CTCAGTGCCA ACCTGGAAAA GATAATAACC ATCGGCCTGT 
     2761 TCTTCTCCAA TTTTGAGCGA AACCCACCCG AGAACACCTT CCTTAGACTC ACCGCCATGG 
     2821 CAACACACTC TGAATCCAAC CTTAGCTGCT TTGCTCAGGA AGACATTGCC ATTTGTAGAC 
     2881 CACACCTTGC CATCAAGATG CCAGAGAAAG CAGAGCAGTA TCAACCCCTC ACAGCCTCAG 
     2941 TCAGCCTCCA GAACTCCCTA GATGCCCCCA TGGAGGACTG TGTGATCTCC ATCCTGGGAA 
     3001 GGGGGCTCAT TCACAGAGAG AGGAGCTACA GATTCCGTTC AGTGTGGCCT GAAAACACCA 
     3061 TGTGTGCCAA GTTCCAGTTC ACGCCAACAC ATGTGGGGCT CCAGAGACTC ACTGTGGAAG 
     3121 TGGACTGCAA CATGTTCCAG AACCTAACCA ACTATAAAAG CGTCACCGTG GTAGCCCCTG 
     3181 AACTATCAGC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3241 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3301 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3361 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3421 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3481 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3541 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3601 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3661 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3721 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3781 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3841 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3901 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3961 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4021 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4081 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4861 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4921 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4981 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5041 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5101 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5161 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5221 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5281 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5341 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5401 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5461 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5521 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5581 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5641 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5701 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5761 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5821 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5881 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5941 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6001 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6061 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6121 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6181 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6241 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6301 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6361 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6421 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6481 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6541 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6601 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6661 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6721 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6781 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6841 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6901 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6961 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7021 TAACAAAAAT TTAACGCGAA TTTT
//