LOCUS       RC211579                7557 bp    DNA     circular  SYN 
DEFINITION  ZNF512B (Myc-DDK-tagged)-Human zinc finger protein 512B (ZNF512B)
ACCESSION   NM_020713
VERSION     NM_020713.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3714..3805 
                     /label="Myc-DDK"  
     misc_feature    3835..4420 
                     /label="PolyA signal"  
     misc_feature    3892..3911 
                     /label="Restriction Enzyme"  
     misc_feature    4696..5368 
                     /label="ColE1"  
     misc_feature    5798..6592 
                     /label="Kan/Neo"  
     misc_feature    6627..6929 
                     /label="SV40 ori"  
     misc_feature    7015..7492 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3702 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3696 
                     /label="ZNF512B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGATCCT TTCTGCGTTG GAGGCCGTCG GCTCCCGGGG TCCAGCAAGA 
     1081 GTGGTCCCGG GAAGGATGGC AGCCGAAAGG AGGTCCGACT TCCAATGCTG CATGACCCAC 
     1141 CGAAGATGGG GATGCCGGTG GTCCGTGGTG GACAGACAGT GCCCGGCCAG GCCCCTCTCT 
     1201 GCTTTGACCC GGGAAGTCCA GCCAGTGACA AGACAGAAGG GAAGAAAAAG GGGCGGCCAA 
     1261 AAGCCGAGAA CCAGGCCCTC CGAGACATTC CTCTCTCCCT GATGAACGAC TGGAAGGATG 
     1321 AGTTCAAGGC ACACTCGAGG GTGAAGTGTC CAAACTCAGG GTGCTGGCTG GAGTTCCCCA 
     1381 GCATCTACGG GCTCAAGTAC CATTACCAGC GGTGCCAAGG GGGTGCCATC TCAGATCGCC 
     1441 TGGCCTTCCC CTGCCCCTTC TGCGAGGCCG CATTCACCTC TAAGACCCAG CTGGAGAAAC 
     1501 ACCGGATCTG GAACCACATG GACCGACCCC TGCCTGCCTC CAAGCCTGGG CCCATCAGCA 
     1561 GGCCGGTCAC CATCAGCCGG CCTGTTGGGG TCAGCAAGCC CATCGGAGTG AGCAAACCTG 
     1621 TCACTATTGG CAAACCTGTG GGTGTCAGCA AACCCATTGG CATCAGCAAG CCAGTCTCGG 
     1681 TCGGCAGACC CATGCCAGTC ACCAAGGCCA TCCCGGTCAC TAGGCCCGTG CCAGTCACCA 
     1741 AACCTGTCAC AGTCAGCAGG CCCATGCCCG TCACCAAGGC CATGCCGGTC ACCAAACCCA 
     1801 TCACAGTCAC CAAGTCTGTG CCGGTCACCA AACCCGTACC TGTCACCAAA CCCATTACGG 
     1861 TCACAAAGCT TGTGACAGTT ACGAAACCCG TGCCGGTCAC CAAGCCAGTG ACAGTCAGCA 
     1921 GGCCCATTGT GGTCAGCAAG CCGGTGACAG TCAGCAGGCC CATTGCTATC AGCAGACACA 
     1981 CACCGCCCTG CAAAATGGTG CTGCTGACCA GGTCGGAGAA CAAAGCACCT CGTGCCACAG 
     2041 GGAGGAACAG TGGTAAGAAA AGGGCTGCGG ACAGCCTGGA CACCTGCCCA ATTCCACCCA 
     2101 AGCAGGCCAG GCCAGAGAAT GGGGAGTACG GCCCCTCCTC CATGGGCCAG AGCTCGGCCT 
     2161 TCCAGCTGAG TGCAGACACC AGCAGTGGCT CCTTGTCGCC AGGCAGCAGG CCGTCAGGGG 
     2221 GCATGGAGGC ACTGAAGGCT GCAGGCCCTG CGTCCCCGCC TGAGGAGGAC CCGGAGCGCA 
     2281 CAAAGCACAG AAGGAAACAG AAAACACCCA AAAAGTTTAC AGGGGAGCAG CCATCCATCT 
     2341 CAGGGACCTT TGGGCTCAAA GGCCTGGTCA AAGCTGAGGA CAAGGCCCGA GTTCACCGCT 
     2401 CCAAGAAGCA GGAGGGGCCA GGCCCTGAGG ACGCCCGGAA GAAGGTGCCA GCTGCCCCCA 
     2461 TCACTGTCAG CAAGGAGGCA CCGGCCCCTG TGGCCCACCC AGCTCCAGGT GGCCCTGAAG 
     2521 AGCAGTGGCA GAGGGCCATC CATGAGCGCG GGGAAGCCGT CTGCCCCACC TGCAACGTGG 
     2581 TCACCCGGAA GACTCTCGTG GGGCTCAAGA AGCACATGGA GGTGTGTCAG AAGCTTCAGG 
     2641 ATGCACTCAA GTGCCAGCAC TGCCGGAAGC AGTTCAAGTC CAAAGCCGGC CTCAACTACC 
     2701 ACACTATGGC CGAGCACAGT GCCAAGCCCT CTGACGCCGA GGCCTCCGAA GGGGGCGAGC 
     2761 AGGAGGAGCG CGAGAGGCTG CGCAAGGTGC TGAAGCAGAT GGGACGGCTG CGCTGCCCCC 
     2821 AGGAGGGTTG CGGGGCTGCC TTCTCCAGCC TCATGGGCTA CCAGTACCAC CAGCGGCGCT 
     2881 GCGGGAAGCC GCCCTGCGAG GTGGACAGCC CCTCCTTCCC CTGCACCCAC TGTGGCAAGA 
     2941 CGTACCGATC CAAGGCTGGC CACGACTACC ACGTGCGCTC GGAGCACACG GCCCCCCCCC 
     3001 CTGAGGAGCC CACAGACAAG TCCCCTGAGG CTGAGGACCC GCTGGGTGTG GAGCGGACCC 
     3061 CAAGCGGGCG TGTCCGCCGC ACGTCGGCCC AGGTGGCGGT GTTCCACCTG CAGGAGATAG 
     3121 CGGAGGACGA GCTGGCCCGC GACTGGACCA AGCGGCGCAT GAAGGATGAC CTTGTGCCCG 
     3181 AGACCGCACG GCTCAACTAC ACTCGACCAG GGCTCCCCAC GCTGAACCCC CAGCTGCTAG 
     3241 AGGCATGGAA GAATGAAGTG AAGGAGAAAG GCCACGTCAA CTGTCCCAAC GACTGCTGTG 
     3301 AAGCCATCTA CTCCAGCGTG TCCGGACTCA AGGCTCATCT CGCCAGCTGC AGTAAGGGGG 
     3361 CCCACCTGGC AGGGAAGTAC CGCTGTCTGC TGTGTCCGAA GGAGTTCAGT TCTGAGAGTG 
     3421 GCGTCAAATA CCACATCCTG AAGACCCACG CAGAGAACTG GTTCCGAACA TCAGCAGACC 
     3481 CACCTCCCAA ACACAGGAGC CAGGACTCAT TGGTGCCCAA GAAGGAAAAG AAGAAAAATC 
     3541 TGGCAGGTGG AAAGAAGCGG GGCCGAAAGC CCAAGGAGCG GACCCCAGAG GAGCCTGTGG 
     3601 CCAAGCTGCC CCCGCGCCGG GACGACTGGC CTCCAGGATG CAGAGACAAG GGGGCCCGGG 
     3661 GCTCCACCGG CCGGAAGGTG GGAGTCAGCA AGGCGCCTGA AAAGACGCGT ACGCGGCCGC 
     3721 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3781 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3841 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3901 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3961 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4021 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4081 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4141 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4201 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4261 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4321 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4381 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4441 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4501 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4561 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4621 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4681 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4741 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4801 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4861 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4921 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4981 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5041 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5101 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5161 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5221 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5281 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5341 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5401 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5461 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5521 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5581 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5641 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5701 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5761 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5821 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5881 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5941 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6001 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6061 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6121 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6181 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6241 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6301 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6361 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6421 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6481 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6541 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6601 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6661 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6721 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6781 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6841 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6901 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6961 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7021 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7081 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7141 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7201 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7261 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7321 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7381 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7441 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7501 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//