LOCUS       RC211511                7212 bp    DNA     circular  SYN 
DEFINITION  BARD1 (Myc-DDK-tagged)-Human BRCA1 associated RING domain 1 (BARD1)
ACCESSION   NM_000465
VERSION     NM_000465.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3369..3460 
                     /label="Myc-DDK"  
     misc_feature    3490..4075 
                     /label="PolyA signal"  
     misc_feature    3547..3566 
                     /label="Restriction Enzyme"  
     misc_feature    4351..5023 
                     /label="ColE1"  
     misc_feature    5453..6247 
                     /label="Kan/Neo"  
     misc_feature    6282..6584 
                     /label="SV40 ori"  
     misc_feature    6670..7147 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3351..3357 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3351 
                     /label="BARD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGATAAT CGGCAGCCGA GGAACCGGCA GCCGAGGATC CGCTCCGGGA 
     1081 ACGAGCCTCG TTCCGCGCCC GCCATGGAAC CGGATGGTCG CGGTGCCTGG GCCCACAGTC 
     1141 GCGCCGCGCT CGACCGCCTG GAGAAGCTGC TGCGCTGCTC GCGTTGTACT AACATTCTGA 
     1201 GAGAGCCTGT GTGTTTAGGA GGATGTGAGC ACATCTTCTG TAGTAATTGT GTAAGTGACT 
     1261 GCATTGGAAC TGGATGTCCA GTGTGTTACA CCCCGGCCTG GATACAAGAC TTGAAGATAA 
     1321 ATAGACAACT GGACAGCATG ATTCAACTTT GTAGTAAGCT TCGAAATTTG CTACATGACA 
     1381 ATGAGCTGTC AGATTTGAAA GAAGATAAAC CTAGGAAAAG TTTGTTTAAT GATGCAGGAA 
     1441 ACAAGAAGAA TTCAATTAAA ATGTGGTTTA GCCCTCGAAG TAAGAAAGTC AGATATGTTG 
     1501 TGAGTAAAGC TTCAGTGCAA ACCCAGCCTG CAATAAAAAA AGATGCAAGT GCTCAGCAAG 
     1561 ACTCATATGA ATTTGTTTCC CCAAGTCCTC CTGCAGATGT TTCTGAGAGG GCTAAAAAGG 
     1621 CTTCTGCAAG ATCTGGAAAA AAGCAAAAAA AGAAAACTTT AGCTGAAATC AACCAAAAAT 
     1681 GGAATTTAGA GGCAGAAAAA GAAGATGGTG AATTTGACTC CAAAGAGGAA TCTAAGCAAA 
     1741 AGCTGGTATC CTTCTGTAGC CAACCATCTG TTATCTCCAG TCCTCAGATA AATGGTGAAA 
     1801 TAGACTTACT AGCAAGTGGC TCCTTGACAG AATCTGAATG TTTTGGAAGT TTAACTGAAG 
     1861 TCTCTTTACC ATTGGCTGAG CAAATAGAGT CTCCAGACAC TAAGAGCAGG AATGAAGTAG 
     1921 TGACTCCTGA GAAGGTCTGC AAAAATTATC TTACATCTAA GAAATCTTTG CCATTAGAAA 
     1981 ATAATGGAAA ACGTGGCCAT CACAATAGAC TTTCCAGTCC CATTTCTAAG AGATGTAGAA 
     2041 CCAGCATTCT GAGCACCAGT GGAGATTTTG TTAAGCAAAC GGTGCCCTCA GAAAATATAC 
     2101 CATTGCCTGA ATGTTCTTCA CCACCTTCAT GCAAACGTAA AGTTGGTGGT ACATCAGGGA 
     2161 GCAAAAACAG TAACATGTCC GATGAATTCA TTAGTCTTTC ACCAGGTACA CCACCTTCTA 
     2221 CATTAAGTAG TTCAAGTTAC AGGCGAGTGA TGTCTAGTCC CTCAGCAATG AAGCTGTTGC 
     2281 CCAATATGGC TGTGAAAAGA AATCATAGAG GAGAGACTTT GCTCCATATT GCTTCTATTA 
     2341 AGGGCGACAT ACCTTCTGTT GAATACCTTT TACAAAATGG AAGTGATCCA AATGTTAAAG 
     2401 ACCATGCTGG ATGGACACCA TTGCATGAAG CTTGCAATCA TGGGCACCTG AAGGTAGTGG 
     2461 AATTATTGCT CCAGCATAAG GCATTGGTGA ACACCACCGG GTATCAAAAT GACTCACCAC 
     2521 TTCACGATGC AGCCAAGAAT GGGCACGTGG ATATAGTCAA GCTGTTACTT TCCTATGGAG 
     2581 CCTCCAGAAA TGCTGTTAAT ATATTTGGTC TGCGGCCTGT CGATTATACA GATGATGAAA 
     2641 GTATGAAATC GCTATTGCTG CTACCAGAGA AGAATGAATC ATCCTCAGCT AGCCACTGCT 
     2701 CAGTAATGAA CACTGGGCAG CGTAGGGATG GACCTCTTGT ACTTATAGGC AGTGGGCTGT 
     2761 CTTCAGAACA ACAGAAAATG CTCAGTGAGC TTGCAGTAAT TCTTAAGGCT AAAAAATATA 
     2821 CTGAGTTTGA CAGTACAGTA ACTCATGTTG TTGTTCCTGG TGATGCAGTT CAAAGTACCT 
     2881 TGAAGTGTAT GCTTGGGATT CTCAATGGAT GCTGGATTCT AAAATTTGAA TGGGTAAAAG 
     2941 CATGTCTACG AAGAAAAGTA TGTGAACAGG AAGAAAAGTA TGAAATTCCT GAAGGTCCAC 
     3001 GCAGAAGCAG GCTCAACAGA GAACAGCTGT TGCCAAAGCT GTTTGATGGA TGCTACTTCT 
     3061 ATTTGTGGGG AACCTTCAAA CACCATCCAA AGGACAACCT TATTAAGCTC GTCACTGCAG 
     3121 GTGGGGGCCA GATCCTCAGT AGAAAGCCCA AGCCAGACAG TGACGTGACT CAGACCATCA 
     3181 ATACAGTCGC ATACCATGCG AGACCCGATT CTGATCAGCG CTTCTGCACA CAGTATATCA 
     3241 TCTATGAAGA TTTGTGTAAT TATCACCCAG AGAGGGTTCG GCAGGGCAAA GTCTGGAAGG 
     3301 CTCCTTCGAG CTGGTTTATA GACTGTGTGA TGTCCTTTGA GTTGCTTCCT CTTGACAGCA 
     3361 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3421 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3481 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3541 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3601 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3661 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3721 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3781 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3841 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3901 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3961 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4021 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4081 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4141 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4201 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4261 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4321 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4381 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4441 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4501 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4561 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4621 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4681 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4741 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4801 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4861 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4921 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4981 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5041 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5101 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5161 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5221 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5281 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5341 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5401 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5461 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5521 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5581 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5641 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5701 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5761 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5821 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5881 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5941 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6001 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6061 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6121 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6181 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6241 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6301 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6361 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6421 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6481 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6541 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6601 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6661 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6721 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6781 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6841 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6901 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7021 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7081 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7141 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7201 AACGCGAATT TT
//