LOCUS       RC211386                7995 bp    DNA     circular  SYN 
DEFINITION  MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1
ACCESSION   NM_015134
VERSION     NM_015134.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4152..4243 
                     /label="Myc-DDK"  
     misc_feature    4273..4858 
                     /label="PolyA signal"  
     misc_feature    4330..4349 
                     /label="Restriction Enzyme"  
     misc_feature    5134..5806 
                     /label="ColE1"  
     misc_feature    6236..7030 
                     /label="Kan/Neo"  
     misc_feature    7065..7367 
                     /label="SV40 ori"  
     misc_feature    7453..7930 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4134..4140 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4134 
                     /label="MPRIP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCAGCC AAGGAGAACC CGTGCAGGAA ATTCCAGGCC AACATCTTCA 
     1081 ACAAGAGCAA GTGTCAGAAC TGCTTCAAGC CCCGCGAGTC GCATCTGCTC AACGACGAGG 
     1141 ACCTGACGCA GGCAAAACCC ATTTATGGCG GTTGGCTGCT CCTGGCTCCA GATGGGACCG 
     1201 ACTTTGACAA CCCAGTGCAC CGGTCTCGGA AATGGCAGCG ACGGTTCTTC ATCCTTTACG 
     1261 AGCACGGCCT CTTGCGCTAC GCCCTGGATG AGATGCCCAC GACCCTTCCT CAGGGCACCA 
     1321 TCAACATGAA CCAGTGCACA GATGTGGTGG ATGGGGAGGG CCGCACGGGC CAGAAGTTCT 
     1381 CCCTGTGTAT TCTGACGCCT GAGAAGGAGC ATTTCATCCG GGCGGAGACC AAGGAGATCG 
     1441 TCAGTGGGTG GCTGGAGATG CTCATGGTCT ATCCCCGGAC CAACAAGCAG AATCAGAAGA 
     1501 AGAAACGGAA AGTGGAGCCC CCCACACCAC AGGAGCCTGG GCCTGCCAAG GTGGCTGTTA 
     1561 CCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCATCCC CAGTGCTGAG AAAGTCCCCA 
     1621 CCACCAAGTC CACACTCTGG CAGGAAGAAA TGAGGACCAA GGACCAGCCA GATGGCAGCA 
     1681 GCCTGAGTCC AGCTCAGAGT CCCAGCCAGA GCCAGCCTCC TGCTGCCAGC TCCCTGCGGG 
     1741 AACCTGGGCT AGAGAGCAAA GAAGAGGAGA GCGCCATGAG TAGCGACCGC ATGGACTGTG 
     1801 GCCGCAAAGT CCGGGTGGAG AGCGGCTACT TCTCTCTGGA GAAGACCAAA CAGGACTTGA 
     1861 AGGCTGAAGA ACAGCAGCTG CCCCCGCCGC TCTCCCCTCC CAGCCCCAGC ACCCCCAACC 
     1921 ACAGGAGGTC CCAGGTGATT GAAAAGTTTG AGGCCTTGGA CATTGAGAAG GCAGAGCACA 
     1981 TGGAGACCAA TGCAGTGGGG CCCTCACCAT CCAGCGACAC ACGCCAGGGC CGCAGCGAGA 
     2041 AGAGGGCGTT CCCTAGGAAG CGGGACTTCA CCAATGAAGC CCCCCCAGCT CCTCTCCCAG 
     2101 ACGCCTCGGC TTCCCCCCTG TCTCCACACC GAAGAGCCAA GTCACTGGAC AGGAGGTCCA 
     2161 CGGAGCCCTC CGTGACGCCC GACCTGCTGA ATTTCAAGAA AGGCTGGCTG ACTAAGCAGT 
     2221 ATGAGGACGG CCAGTGGAAG AAACACTGGT TTGTCCTCGC CGATCAAAGC CTGAGATACT 
     2281 ACAGGGATTC AGTGGCTGAG GAGGCAGCCG ACTTGGATGG AGAAATTGAC TTGTCCGCAT 
     2341 GTTACGATGT CACAGAGTAT CCAGTTCAGA GAAACTATGG CTTCCAGATA CATACAAAGG 
     2401 AGGGCGAGTT TACCCTGTCG GCCATGACAT CTGGGATTCG GCGGAACTGG ATCCAGACCA 
     2461 TCATGAAGCA CGTGCACCCG ACCACTGCCC CGGATGTGAC CAGCTCGTTG CCAGAGGAAA 
     2521 AAAACAAGAG CAGCTGCTCT TTTGAGACCT GCCCGAGGCC TACTGAGAAG CAAGAGGCAG 
     2581 AGCTGGGGGA GCCGGACCCT GAGCAGAAGA GGAGCCGCGC ACGGGAGCGG AGGCGAGAGG 
     2641 GCCGCTCCAA GACCTTTGAC TGGGCTGAGT TCCGTCCCAT CCAGCAGGCC CTGGCTCAGG 
     2701 AGCGGGTGGG CGGCGTGGGG CCTGCTGACA CCCACGAGCC CCTGCGCCCT GAGGCGGAGC 
     2761 CTGGGGAGCT GGAGCGGGAG CGTGCACGGA GGCGGGAGGA GCGCCGCAAG CGCTTCGGGA 
     2821 TGCTCGACGC CACAGACGGG CCAGGCACTG AGGATGCAGC CCTGCGCATG GAGGTGGACC 
     2881 GGAGCCCAGG GCTGCCTATG AGCGACCTCA AAACGCATAA CGTCCACGTG GAGATTGAGC 
     2941 AGCGGTGGCA TCAGGTGGAG ACCACACCTC TCCGGGAAGA GAAGCAGGTG CCCATCGCCC 
     3001 CCGTCCACCT GTCTTCTGAA GATGGGGGTG ACCGGCTCTC CACACACGAG CTGACCTCTC 
     3061 TGCTCGAGAA GGAGCTGGAG CAGAGCCAGA AGGAGGCCTC AGACCTTCTG GAGCAGAACC 
     3121 GGCTCCTGCA GGACCAGCTG AGGGTGGCCC TGGGCCGGGA GCAGAGCGCC CGTGAGGGCT 
     3181 ACGTGCTGCA GGCCACGTGC GAGCGAGGGT TTGCAGCAAT GGAAGAAACG CACCAGAAGA 
     3241 AGATTGAAGA TCTCCAGAGG CAGCACCAGC GGGAGCTAGA GAAACTTCGA GAAGAGAAAG 
     3301 ACCGCCTCCT AGCCGAGGAG ACAGCGGCCA CCATCTCAGC CATCGAAGCC ATGAAGAACG 
     3361 CCCACCGGGA GGAAATGGAG CGGGAGCTGG AGAAGAGCCA GCGGTCCCAG ATCAGCAGCG 
     3421 TCAACTCGGA TGTTGAGGCC CTGCGGCGCC AGTACCTGGA GGAGCTGCAG TCGGTGCAGC 
     3481 GGGAACTGGA GGTCCTCTCG GAGCAGTACT CGCAGAAGTG CCTGGAGAAT GCCCATCTGG 
     3541 CCCAGGCGCT GGAGGCCGAG CGGCAGGCCC TGCGGCAGTG CCAGCGTGAG AACCAGGAGC 
     3601 TCAATGCCCA CAACCAGGAG CTGAACAACC GCCTGGCTGC AGAGATCACA CGGTTGCGGA 
     3661 CGCTGCTGAC TGGGGACGGC GGTGGGGAGG CCACTGGGTC ACCCCTTGCA CAGGGCAAGG 
     3721 ATGCCTATGA ACTAGAGGTC TTATTGCGGG TAAAGGAATC GGAAATACAG TACCTGAAAC 
     3781 AGGAGATTAG CTCCCTCAAG GATGAGCTGC AGACGGCACT GCGGGACAAG AAGTACGCAA 
     3841 GTGACAAGTA CAAAGACATC TACACAGAGC TCAGCATCGC GAAGGCTAAG GCTGACTGTG 
     3901 ACATCAGCAG GTTGAAGGAG CAGCTCAAGG CTGCAACGGA AGCACTGGGG GAGAAGTCCC 
     3961 CTGACAGTGC CACGGTGTCC GGATATGATA TAATGAAATC TAAAAGCAAC CCTGACTTCT 
     4021 TGAAGAAAGA CAGATCCTGT GTCACCCGGC AACTCAGAAA CATCAGGTCC AAGAGTCTGA 
     4081 AGGAAGGCCT GACGGTGCAA GAACGGTTGA AGCTCTTTGA ATCCAGGGAC TTGAAGAAAG 
     4141 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4201 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4261 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4321 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4381 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4441 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4501 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4561 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4621 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4681 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4741 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4801 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4861 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4921 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4981 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5041 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5641 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5701 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5761 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5821 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5881 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5941 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6001 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6061 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6121 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6181 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6241 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6301 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6361 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6421 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6481 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6541 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6601 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6661 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6721 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6781 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6841 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6901 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6961 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7021 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7081 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7141 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7201 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7321 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7381 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7441 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7501 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7561 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7621 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7681 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7741 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7801 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7861 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7921 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7981 TTTAACGCGA ATTTT
//