LOCUS       RC211375                6990 bp    DNA     circular  SYN 
DEFINITION  GYS2 (Myc-DDK-tagged)-Human glycogen synthase 2 (liver) (GYS2)
ACCESSION   NM_021957
VERSION     NM_021957.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3147..3238 
                     /label="Myc-DDK"  
     misc_feature    3268..3853 
                     /label="PolyA signal"  
     misc_feature    3325..3344 
                     /label="Restriction Enzyme"  
     misc_feature    4129..4801 
                     /label="ColE1"  
     misc_feature    5231..6025 
                     /label="Kan/Neo"  
     misc_feature    6060..6362 
                     /label="SV40 ori"  
     misc_feature    6448..6925 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3129..3135 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3129 
                     /label="GYS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTCGAGGC CGATCCCTCT CTGTAACATC CCTGGGTGGG CTTCCCCAGT 
     1081 GGGAAGTCGA AGAACTTCCT GTGGAGGAGT TACTGCTCTT TGAAGTTGCT TGGGAAGTGA 
     1141 CCAATAAAGT TGGAGGCATC TATACTGTGA TTCAGACAAA GGCCAAAACA ACAGCAGATG 
     1201 AATGGGGAGA GAACTATTTT CTGATAGGTC CATATTTTGA GCATAATATG AAGACTCAGG 
     1261 TGGAACAGTG TGAACCTGTA AATGATGCTG TCAGAAGAGC AGTGGACGCA ATGAATAAGC 
     1321 ATGGCTGCCA GGTGCATTTT GGAAGATGGC TGATAGAAGG AAGTCCTTAT GTGGTACTTT 
     1381 TTGACATAGG CTATTCAGCT TGGAATCTGG ACAGGTGGAA GGGTGACCTC TGGGAAGCAT 
     1441 GCAGTGTCGG CATTCCTTAT CATGACCGAG AAGCCAATGA TATGCTGATA TTTGGATCTT 
     1501 TAACTGCCTG GTTCTTAAAA GAGGTGACAG ATCATGCAGA TGGTAAATAT GTCGTTGCCC 
     1561 AATTCCATGA ATGGCAGGCT GGAATTGGAC TGATCCTTTC TCGAGCCAGG AAACTTCCTA 
     1621 TTGCCACAAT ATTTACAACC CACGCTACAC TACTTGGGAG GTATCTCTGT GCAGCAAATA 
     1681 TTGATTTCTA CAACCATCTT GATAAGTTTA ACATTGACAA AGAGGCTGGG GAAAGGCAGA 
     1741 TTTACCACCG GTACTGCATG GAGCGAGCTT CCGTTCATTG CGCTCACGTG TTCACCACGG 
     1801 TTTCTGAAAT AACAGCAATA GAAGCTGAAC ATATGCTGAA GAGAAAGCCT GATGTAGTTA 
     1861 CTCCAAACGG CTTGAATGTT AAGAAATTTT CAGCAGTGCA TGAGTTTCAA AATCTACATG 
     1921 CCATGTACAA GGCCAGAATC CAAGATTTTG TTCGAGGTCA TTTCTATGGT CATCTCGACT 
     1981 TTGATCTTGA AAAGACTTTG TTCCTTTTCA TTGCTGGGAG GTATGAGTTT TCAAACAAAG 
     2041 GAGCTGACAT CTTCCTAGAA TCCTTATCCA GGCTAAATTT CCTGCTGAGG ATGCATAAAA 
     2101 GTGACATCAC AGTGGTGGTG TTTTTCATTA TGCCTGCCAA GACAAATAAT TTCAACGTGG 
     2161 AAACCCTGAA AGGACAAGCA GTGCGAAAAC AGCTGTGGGA TGTTGCACAT TCTGTGAAGG 
     2221 AAAAGTTTGG AAAAAAACTC TATGATGCAT TATTAAGAGG AGAAATTCCT GACCTGAACG 
     2281 ATATTTTAGA TCGAGATGAT CTAACAATTA TGAAAAGAGC CATCTTTTCA ACTCAGCGAC 
     2341 AGTCATTGCC CCCAGTGACC ACGCACAACA TGATTGATGA CTCCACCGAC CCCATCCTCA 
     2401 GCACCATTAG ACGGATTGGA CTTTTCAACA ACCGCACAGA TAGAGTCAAG GTGATTTTGC 
     2461 ACCCAGAGTT TCTATCCTCC ACCAGTCCCT TACTACCCAT GGACTATGAA GAGTTTGTTA 
     2521 GAGGTTGTCA TCTTGGAGTA TTTCCATCAT ACTATGAACC CTGGGGTTAT ACTCCAGCTG 
     2581 AATGCACTGT GATGGGTATC CCCAGTGTGA CCACGAATCT CTCCGGGTTT GGCTGTTTCA 
     2641 TGCAGGAGCA CGTGGCTGAT CCTACTGCTT ACGGTATTTA CATCGTTGAC AGGCGGTTCC 
     2701 GTTCTCCAGA TGATTCTTGC AATCAGCTGA CTAAGTTTCT CTATGGATTT TGCAAACAGT 
     2761 CACGCCGCCA AAGGATTATC CAGAGGAACA GAACTGAGAG GCTCTCAGAT CTTCTGGATT 
     2821 GGAGATACTT AGGCAGATAT TACCAGCATG CCAGACACCT GACATTAAGC AGAGCTTTTC 
     2881 CAGATAAATT CCATGTGGAA CTAACATCAC CACCAACGAC AGAAGGATTT AAATATCCCA 
     2941 GGCCTTCCTC AGTACCACCT TCTCCTTCAG GGTCTCAGGC CTCCAGTCCT CAGAGCAGTG 
     3001 ATGTGGAAGA TGAAGTGGAG GATGAGAGAT ACGATGAGGA AGAGGAGGCT GAAAGGGATC 
     3061 GGTTAAATAT CAAGTCACCA TTTTCACTGA GCCACGTTCC TCATGGGAAG AAAAAGCTGC 
     3121 ATGGTGAATA TAAGAACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3181 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3241 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3301 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3361 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3421 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3481 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3541 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3601 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3661 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3721 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3781 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3841 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3901 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3961 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4021 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4081 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4141 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4201 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4261 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4321 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4381 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4441 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4501 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4561 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4621 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4681 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4741 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4801 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4861 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4921 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4981 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5041 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5101 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5161 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5221 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5281 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5341 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5401 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5461 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5521 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5581 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5641 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5701 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5761 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5821 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5881 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5941 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6001 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6061 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6121 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6181 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6241 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6301 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6361 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6421 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6481 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6541 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6601 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6661 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6721 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6781 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6841 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6901 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6961 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//