LOCUS RC211359 6492 bp DNA circular SYN DEFINITION RIC8A (Myc-DDK-tagged)-Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A) ACCESSION NM_021932 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2649..2740 /label="Myc-DDK" misc_feature 2770..3355 /label="PolyA signal" misc_feature 2827..2846 /label="Restriction Enzyme" misc_feature 3631..4303 /label="ColE1" misc_feature 4733..5527 /label="Kan/Neo" misc_feature 5562..5864 /label="SV40 ori" misc_feature 5950..6427 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2631..2637 /label="Restriction Enzyme" misc_feature 1020..2631 /label="RIC8A" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGAGCCCCGG GCGGTTGCAG AAGCCGTGGA GACGGGTGAG GAGGATGTGA 1081 TTATGGAAGC TCTGCGGTCA TACAACCAGG AGCACTCCCA GAGCTTCACG TTTGATGATG 1141 CCCAACAGGA GGACCGGAAG AGACTGGCGG AGCTGCTGGT CTCCGTCCTG GAACAGGGCT 1201 TGCCACCCTC CCACCGTGTC ATCTGGCTGC AGAGTGTCCG AATCCTGTCC CGGGACCGCA 1261 ACTGCCTGGA CCCGTTCACC AGCCGCCAGA GCCTGCAGGC ACTAGCCTGC TATGCTGACA 1321 TCTCTGTCTC TGAGGGGTCC GTCCCAGAGT CCGCAGACAT GGATGTTGTA CTGGAGTCCC 1381 TCAAGTGCCT GTGCAACCTC GTGCTCAGCA GCCCTGTGGC ACAGATGCTG GCAGCAGAGG 1441 CCCGCCTAGT GGTGAAGCTC ACAGAGCGTG TGGGGCTGTA CCGTGAGAGG AGCTTCCCCC 1501 ACGATGTCCA GTTCTTTGAC TTGCGGCTCC TCTTCCTGCT AACGGCACTC CGCACCGATG 1561 TGCGCCAGCA GCTGTTTCAG GAGCTGAAAG GAGTGCGCCT GCTAACTGAC ACACTGGAGC 1621 TGACGCTGGG GGTGACTCCT GAAGGGAACC CCCCACCCAC GCTCCTTCCT TCCCAAGAGA 1681 CTGAGCGGGC CATGGAGATC CTCAAAGTGC TCTTCAACAT CACCCTGGAC TCCATCAAGG 1741 GGGAGGTGGA CGAGGAAGAC GCTGCCCTTT ACCGACACCT GGGGACCCTT CTCCGGCACT 1801 GTGTGATGAT CGCTACTGCT GGAGACCGCA CAGAGGAGTT CCAGGGCCAC GCAGTGAACC 1861 TCCTGGGGAA CTTGCCCCTC AAGTGTCTGG ATGTTCTCCT CACCCTGGAG CCACATGGAG 1921 ACTCCACGGA GTTCATGGGA GTGAATATGG ATGTGATTCG TGCCCTCCTC ATCTTCCTAG 1981 AGAAGCGTTT GCACAAGACA CACAGGCTGA AGGAGAGTGT AGCTCCCGTG CTGAGCGTGC 2041 TGACTGAATG TGCCCGGATG CACCGCCCAG CCAGGAAGTT CCTGAAGGCC CAGGGATGGC 2101 CACCTCCCCA GGTGCTGCCC CCTCTGCGGG ATGTGAGGAC ACGGCCTGAG GTTGGGGAGA 2161 TGCTGCGGAA CAAGCTTGTC CGCCTCATGA CACACCTGGA CACAGATGTG AAGAGGGTGG 2221 CTGCCGAGTT CTTGTTTGTC CTGTGCTCTG AGAGTGTGCC CCGATTCATC AAGTACACAG 2281 GCTATGGGAA TGCTGCTGGC CTTCTGGCTG CCAGGGGCCT CATGGCAGGA GGCCGGCCCG 2341 AGGGCCAGTA CTCAGAGGAT GAGGACACAG ACACAGATGA GTACAAGGAA GCCAAAGCCA 2401 GCATAAACCC TGTGACCGGG AGGGTGGAGG AGAAGCCGCC TAACCCTATG GAGGGCATGA 2461 CAGAGGAGCA GAAGGAGCAC GAGGCCATGA AGCTGGTGAC CATGTTTGAC AAGCTCTCCA 2521 GGAACAGAGT CATCCAGCCA ATGGGGATGA GTCCCCGGGG TCATCTTACG TCCCTGCAGG 2581 ATGCCATGTG CGAGACTATG GAGCAGCAGC TCTCCTCGGA CCCTGACTCG GACCCTGACA 2641 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 2701 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 2761 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 2821 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 2881 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 2941 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 3001 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 3061 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 3121 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 3181 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 3241 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 3301 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 3361 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 3421 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 3481 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 3541 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 3601 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3661 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 3721 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 3781 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 3841 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 3901 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3961 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 4021 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 4081 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 4141 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 4201 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 4261 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 4321 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 4381 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 4441 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 4501 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 4561 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 4621 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 4681 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 4741 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 4801 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 4861 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 4921 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 4981 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 5041 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 5101 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 5161 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 5221 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 5281 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 5341 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 5401 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 5461 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 5521 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 5581 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 5641 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 5701 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 5761 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 5821 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 5881 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 5941 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 6001 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 6061 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 6121 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 6181 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 6241 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 6301 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 6361 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 6421 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 6481 AACGCGAATT TT //