LOCUS       RC211326                7818 bp    DNA     circular  SYN 
DEFINITION  NEK9 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9)
ACCESSION   NM_033116
VERSION     NM_033116.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3975..4066 
                     /label="Myc-DDK"  
     misc_feature    4096..4681 
                     /label="PolyA signal"  
     misc_feature    4153..4172 
                     /label="Restriction Enzyme"  
     misc_feature    4957..5629 
                     /label="ColE1"  
     misc_feature    6059..6853 
                     /label="Kan/Neo"  
     misc_feature    6888..7190 
                     /label="SV40 ori"  
     misc_feature    7276..7753 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3963 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="NEK9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGCTG GGCGAGTACG AGCGACACTG CGATTCCATC AACTCGGACT 
     1081 TTGGGAGCGA GTCCGGGGGT TGCGGGGACT CGAGTCCGGG GCCTAGCGCC AGTCAGGGGC 
     1141 CGCGAGCCGG CGGCGGCGCG GCGGAGCAGG AGGAACTGCA CTACATCCCC ATCCGCGTCC 
     1201 TGGGCCGCGG CGCCTTCGGG GAAGCCACGC TGTACCGCCG CACCGAGGAT GACTCACTGG 
     1261 TTGTGTGGAA GGAAGTCGAT TTGACCCGGC TGTCTGAGAA GGAACGTCGT GATGCCTTGA 
     1321 ATGAGATAGT TATTCTGGCA CTGCTGCAGC ACGACAACAT TATTGCCTAC TACAATCACT 
     1381 TCATGGACAA TACCACGCTG CTGATTGAGC TGGAATATTG TAATGGAGGG AACCTGTATG 
     1441 ACAAAATCCT TCGTCAGAAG GACAAGTTGT TTGAGGAAGA GATGGTGGTG TGGTACCTAT 
     1501 TTCAGATTGT TTCAGCAGTG AGCTGCATCC ATAAAGCTGG AATCCTTCAT AGAGATATAA 
     1561 AGACATTAAA TATTTTTCTG ACCAAGGCAA ACCTGATAAA ACTTGGAGAT TATGGCCTAG 
     1621 CAAAGAAACT TAATTCTGAG TATTCCATGG CTGAGACGCT TGTGGGAACC CCATATTACA 
     1681 TGTCTCCAGA GCTCTGTCAA GGAGTAAAGT ACAATTTCAA GTCTGATATC TGGGCAGTTG 
     1741 GCTGCGTCAT TTTTGAACTG CTTACCTTAA AGAGGACGTT TGATGCTACA AACCCACTTA 
     1801 ACCTGTGTGT GAAGATCGTG CAAGGAATTC GGGCCATGGA AGTTGACTCT AGCCAGTACT 
     1861 CTTTGGAATT GATCCAAATG GTTCATTCGT GCCTTGACCA GGATCCTGAG CAGAGACCTA 
     1921 CTGCAGATGA ACTTCTAGAT CGCCCTCTTC TCAGGAAACG CAGGAGAGAG ATGGAGGAAA 
     1981 AAGTCACTCT GCTTAATGCA CCTACAAAGA GACCAAGGTC AAGCACTGTG ACTGAAGCAC 
     2041 CCATTGCTGT AGTAACATCA CGAACCAGTG AAGTCTATGT TTGGGGTGGT GGAAAATCCA 
     2101 CCCCCCAGAA ACTGGATGTT ATCAAGAGTG GCTGTAGTGC CCGGCAGGTC TGTGCAGGGA 
     2161 ATACCCACTT TGCTGTGGTC ACAGTGGAGA AGGAACTGTA CACTTGGGTG AACATGCAAG 
     2221 GAGGCACTAA ACTCCATGGT CAGCTGGGCC ATGGAGACAA AGCCTCCTAT CGACAGCCAA 
     2281 AGCATGTGGA AAAGTTGCAA GGCAAAGCTA TCCGTCAGGT GTCATGTGGT GATGATTTCA 
     2341 CTGTCTGTGT GACTGATGAG GGTCAGCTCT ATGCCTTCGG ATCAGATTAT TATGGCTGCA 
     2401 TGGGGGTGGA CAAAGTTGCT GGCCCTGAAG TGCTAGAACC CATGCAGCTG AACTTCTTCC 
     2461 TCAGCAATCC AGTGGAGCAG GTCTCCTGTG GAGATAATCA TGTGGTGGTT CTGACACGAA 
     2521 ACAAGGAAGT CTATTCTTGG GGCTGTGGCG AATATGGACG ACTGGGTTTG GATTCAGAAG 
     2581 AGGATTATTA TACACCACAA AAGGTGGATG TTCCCAAGGC CTTGATTATT GTTGCAGTTC 
     2641 AATGTGGCTG TGATGGGACA TTTCTGTTGA CCCAGTCAGG CAAAGTGCTG GCCTGTGGAC 
     2701 TCAATGAATT CAATAAGCTG GGTCTGAATC AGTGCATGTC GGGAATTATC AACCATGAAG 
     2761 CATACCATGA AGTTCCCTAC ACAACGTCCT TTACCTTGGC CAAACAGTTG TCCTTTTATA 
     2821 AGATCCGTAC CATTGCCCCA GGCAAGACTC ACACAGCTGC TATTGATGAG CGAGGCCGGC 
     2881 TGCTGACCTT TGGCTGCAAC AAGTGTGGGC AGCTGGGCGT TGGGAACTAC AAGAAGCGTC 
     2941 TGGGAATCAA CCTGTTGGGG GGACCCCTTG GTGGGAAGCA AGTGATCAGG GTCTCCTGCG 
     3001 GTGATGAGTT TACCATTGCT GCCACTGATG ATAATCACAT TTTTGCCTGG GGCAATGGTG 
     3061 GTAATGGCCG CCTGGCAATG ACCCCCACAG AGAGACCACA TGGCTCTGAT ATCTGTACCT 
     3121 CATGGCCTCG GCCTATTTTT GGATCTCTGC ATCATGTCCC GGACCTGTCT TGCCGTGGAT 
     3181 GGCATACCAT TCTCATCGTT GAGAAAGTAT TGAATTCTAA GACCATCCGT TCCAATAGCA 
     3241 GTGGCTTATC CATTGGAACT GTGTTTCAGA GCTCTAGCCC GGGAGGAGGC GGCGGGGGCG 
     3301 GCGGTGGTGA AGAAGAGGAC AGTCAGCAGG AATCTGAAAC TCCTGACCCA AGTGGAGGCT 
     3361 TCCGAGGAAC AATGGAAGCA GACCGAGGAA TGGAAGGTTT AATCAGTCCC ACAGAGGCCA 
     3421 TGGGGAACAG TAATGGGGCC AGCAGCTCCT GTCCTGGCTG GCTTCGAAAG GAGCTGGAAA 
     3481 ATGCAGAATT TATCCCCATG CCTGACAGCC CATCTCCTCT CAGTGCAGCG TTTTCAGAAT 
     3541 CTGAGAAAGA TACCCTGCCC TATGAAGAGC TGCAAGGACT CAAAGTGGCC TCTGAAGCTC 
     3601 CTTTGGAACA CAAACCCCAA GTAGAAGCCT CGTCACCTCG GCTGAATCCT GCAGTAACCT 
     3661 GTGCTGGGAA GGGAACACCA CTGACTCCTC CTGCGTGTGC GTGCAGCTCT CTGCAGGTGG 
     3721 AGGTTGAGAG ATTGCAGGGT CTGGTGTTAA AGTGTCTGGC TGAACAACAG AAGCTACAGC 
     3781 AAGAAAACCT CCAGATTTTT ACCCAACTGC AGAAGTTGAA CAAGAAATTA GAAGGAGGGC 
     3841 AGCAGGTGGG GATGCATTCC AAAGGAACTC AGACAGCAAA GGAAGAGATG GAAATGGATC 
     3901 CAAAGCCTGA CTTAGATTCA GATTCCTGGT GCCTCCTGGG AACAGACTCC TGTAGACCCA 
     3961 GCCTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4021 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4081 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4141 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4201 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4261 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4321 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4381 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4441 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4501 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4561 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4621 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4681 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4741 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4801 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4861 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4921 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4981 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5041 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5101 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5161 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5221 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5281 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5341 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5401 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5461 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5521 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5581 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5641 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5701 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5761 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5821 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5881 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5941 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6001 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6061 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6121 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6181 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6241 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6301 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6361 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6421 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6481 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6541 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6601 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6661 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6721 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6781 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6841 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6901 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6961 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7021 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7081 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7141 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7201 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7261 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7321 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7381 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7441 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7501 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7561 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7621 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7681 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7741 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7801 AAATTTAACG CGAATTTT
//