LOCUS       RC211307                8073 bp    DNA     circular  SYN 
DEFINITION  PUM2 (Myc-DDK-tagged)-Human pumilio homolog 2 (Drosophila) (PUM2)
ACCESSION   NM_015317
VERSION     NM_015317.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4230..4321 
                     /label="Myc-DDK"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    4408..4427 
                     /label="Restriction Enzyme"  
     misc_feature    5212..5884 
                     /label="ColE1"  
     misc_feature    6314..7108 
                     /label="Kan/Neo"  
     misc_feature    7143..7445 
                     /label="SV40 ori"  
     misc_feature    7531..8008 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4212..4218 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4212 
                     /label="PUM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCATGAT TTTCAAGCTC TTGCATTAGA ATCTCGGGGA ATGGGAGAGC 
     1081 TTTTGCCTAC CAAAAAGTTT TGGGAACCTG ATGATTCAAC AAAAGATGGA CAAAAAGGCA 
     1141 TATTTCTTGG GGATGATGAA TGGAGAGAGA CTGCATGGGG AGCTTCTCAC CATTCAATGT 
     1201 CCCAGCCTAT TATGGTACAG AGAAGATCTG GACAGGGTTT TCATGGAAAC AGTGAAGTAA 
     1261 ATGCAATACT GTCTCCGCGA TCAGAAAGTG GAGGCCTTGG TGTGAGCATG GTAGAATATG 
     1321 TATTAAGTTC TTCTCCTGCT GATAAATTGG ATTCTCGATT TAGGAAGGGA AATTTTGGCA 
     1381 CTAGAGATGC TGAAACAGAT GGACCTGAGA AAGGAGATCA AAAAGGCAAG GCTTCTCCAT 
     1441 TTGAGGAGGA CCAAAACAGA GATCTTAAAC AAGGAGATGA TGATGATTCT AAAATAAATG 
     1501 GCAGAGGTTT GCCAAATGGA ATGGATGCCG ATTGCAAAGA TTTTAATCGT ACTCCTGGAA 
     1561 GTCGTCAAGC CTCTCCAACT GAAGTAGTTG AGCGCTTGGG CCCCAATACT AATCCCTCAG 
     1621 AAGGACTGGG GCCTCTTCCT AATCCTACAG CTAATAAACC ACTTGTTGAA GAATTTTCAA 
     1681 ATCCTGAAAC TCAGAATCTG GATGCCATGG AACAAGTTGG TCTGGAATCC TTACAGTTTG 
     1741 ACTATCCTGG TAATCAGGTA CCAATGGACT CTTCAGGAGC TACTGTAGGC CTTTTTGACT 
     1801 ACAATTCCCA GCAGCAGCTC TTTCAGAGGA CTAATGCACT AACAGTTCAA CAGTTAACTG 
     1861 CAGCTCAACA GCAGCAATAT GCATTAGCAG CAGCTCAGCA GCCACATATA GCTGGTGTAT 
     1921 TCTCAGCAGG CCTTGCTCCA GCTGCATTTG TGCCAAATCC ATACATTATT AGTGCTGCTC 
     1981 CTCCAGGGAC CGATCCGTAT ACTGCAGCAG GATTGGCTGC AGCAGCTACA TTAGCAGGTC 
     2041 CAGCAGTGGT TCCACCTCAG TATTACGGCG TTCCATGGGG GGTGTATCCA GCCAACTTAT 
     2101 TTCAGCAGCA AGCTGCAGCT GCGGCAAATA ACACAGCCAG TCAGCAAGCA GCATCACAAG 
     2161 CTCAGCCTGG ACAGCAACAG GTTCTCCGTG CTGGAGCAGG TCAGCGTCCT CTTACTCCCA 
     2221 ATCAGGGTCA GCAAGGGCAG CAAGCAGAAT CACTTGCGGC AGCTGCAGCA GCAAATCCAA 
     2281 CATTGGCTTT TGGTCAGGGT CTTGCTACTG GCATGCCAGG CTATCAAGTA CTAGCTCCAA 
     2341 CTGCCTATTA TGATCAGACT GGTGCCTTAG TGGTTGGCCC TGGAGCAAGG ACTGGCCTTG 
     2401 GAGCTCCAGT TCGGTTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA GCAGCACAAG 
     2461 CTGCAGCAGC AGCAGCAGCT GGAGGAACTG CAAGTAGCCT TACAGGCAGC ACAAATGGTC 
     2521 TGTTTCGGCC AATTGGCACT CAGCCACCAC AGCAGCAGCA ACAGCAGCCA AGCACTAATC 
     2581 TGCAATCTAA TTCATTTTAT GGAAGCAGTT CTTTGACTAA TAGCTCCCAG AGTAGTTCTT 
     2641 TATTTTCTCA TGGACCTGGT CAACCTGGAA GTACATCTCT TGGCTTTGGA AGTGGTAACT 
     2701 CTTTGGGTGC TGCTATAGGC TCAGCCCTCA GTGGATTTGG TTCATCAGTT GGCAGTTCTG 
     2761 CAAGTAGTAG TGCCACAAGG AGAGAGTCTC TATCTACTAG CTCTGACTTG TACAAAAGAT 
     2821 CTAGTAGCAG CCTAGCACCC ATAGGGCAAC CATTTTACAA TAGTCTGGGA TTTTCCTCCT 
     2881 CTCCAAGTCC AATAGGCATG CCTCTGCCAA GCCAAACTCC AGGACATTCA CTTACGCCAC 
     2941 CGCCATCACT TTCATCACAT GGATCCTCAT CCAGTTTGCA TTTAGGAGGA CTGACAAATG 
     3001 GTAGTGGTCG ATATATCTCT GCAGCACCTG GAGCAGAAGC AAAATATCGA AGTGCTTCAA 
     3061 GCACTTCCAG TCTATTTAGC TCCAGCAGCC AGCTCTTTCC TCCTTCCCGG CTTCGGTATA 
     3121 ATAGGTCTGA TATTATGCCT TCTGGCCGCA GTAGATTATT GGAAGATTTC AGAAACAACC 
     3181 GCTTCCCAAA CCTTCAGCTT AGAGACTTGA TTGGACATAT AGTTGAGTTT TCTCAAGACC 
     3241 AGCATGGTTC TAGATTCATA CAGCAAAAAC TAGAGAGAGC TACTCCAGCT GAGCGACAGA 
     3301 TGGTATTTAA TGAAATTCTG CAAGCAGCCT ATCAATTAAT GACTGATGTT TTTGGCAACT 
     3361 ATGTTATACA GAAGTTTTTT GAGTTTGGGA GTCTGGATCA AAAATTAGCC CTGGCTACTC 
     3421 GTATTCGTGG TCATGTTCTA CCCTTAGCCT TGCAGATGTA TGGCTGCCGC GTTATTCAGA 
     3481 AAGCATTAGA ATCTATTTCT TCTGACCAGC AGAGTGAAAT GGTAAAGGAG CTGGATGGTC 
     3541 ATGTGCTCAA ATGTGTGAAA GATCAGAATG GAAACCATGT TGTACAAAAA TGTATCGAAT 
     3601 GTGTTCAGCC ACAGTCACTA CAGTTCATCA TTGATGCTTT CAAGGGACAA GTATTTGTGC 
     3661 TTTCAACTCA TCCTTATGGC TGCAGAGTAA TTCAGCGCAT CCTAGAGCAT TGCACTGCAG 
     3721 AACAGACCTT ACCTATCTTA GAAGAACTCC ACCAACATAC AGAGCAGTTG GTACAGGATC 
     3781 AGTATGGCAA TTATGTTATT CAGCATGTAC TGGAACACGG TCGACCTGAA GACAAGAGCA 
     3841 AAATTGTTTC CGAAATCAGG GGAAAGGTTT TAGCCCTGAG TCAACACAAA TTTGCCAGCA 
     3901 ATGTAGTAGA AAAGTGTGTT ACTCATGCCT CCCGTGCTGA GAGAGCTTTA CTGATTGACG 
     3961 AGGTTTGCTG CCAGAATGAT GGTCCTCACA GTGCCTTATA CACCATGATG AAGGACCAGT 
     4021 ATGCCAATTA CGTGGTTCAA AAGATGATTG ATATGGCTGA ACCTGCTCAG AGAAAGATAA 
     4081 TCATGCACAA GATTCGACCT CACATTACTA CTTTGCGCAA ATACACATAC GGGAAGCATA 
     4141 TACTGGCCAA GTTGGAAAAG TATTATTTGA AGAATAGCCC GGACCTAGGA CCTATTGGAG 
     4201 GACCACCAAA TGGAATGCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//